REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS

JENA BP, GUMKOWSKI FD, KONIECZKO EM, Fischer von Mollard G, JAHN R, JAMIESON JD (1994)
JOURNAL OF CELL BIOLOGY 124(1): 43-53.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ;
Abstract
Regulated secretion from pancreatic acinar cells occurs by exocytosis of zymogen granules (ZG) at the apical plasmalemma. ZGs originate from the TGN and undergo prolonged maturation and condensation. After exocytosis, the zymogen granule membrane (ZGM) is retrieved from the plasma membrane and ultimately reaches the TGN. In this study, we analyzed the fate of a low M(r) GTP-binding protein during induced exocytosis and membrane retrieval using immunoblots as well as light and electron microscopic immunocytochemistry. This 27-kD protein, identified by a monoclonal antibody that recognizes rab3A and B, may be a novel rab3 isoform. In resting acinar cells, the rab3-like protein was detected primarily on the cytoplasmic face of ZGs, with little labeling of the Golgi complex and no significant labeling of the apical plasmalemma or any other intracellular membranes. Stimulation of pancreatic lobules in vitro by carbamylcholine for 15 min, resulted in massive exocytosis that led to a near doubling of the area of the apical plasma membrane. However, no relocation of the rab3-like protein to the apical plasmalemma was seen. After 3 h of induced exocytosis, during which time approximately 90% of the ZGs is released, the rab3-like protein appeared to translocate to small vesicles and newly forming secretory granules in the TGN. No significant increase of the rab3-like protein was found in the cytosolic fraction at any time during stimulation. Since the protein is not detected on the apical plasmalemma after stimulation, we conclude that recycling may involve a membrane dissociation-association cycle that accompanies regulated exocytosis.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

JENA BP, GUMKOWSKI FD, KONIECZKO EM, Fischer von Mollard G, JAHN R, JAMIESON JD. REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS. JOURNAL OF CELL BIOLOGY. 1994;124(1):43-53.
JENA, B. P., GUMKOWSKI, F. D., KONIECZKO, E. M., Fischer von Mollard, G., JAHN, R., & JAMIESON, J. D. (1994). REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS. JOURNAL OF CELL BIOLOGY, 124(1), 43-53.
JENA, B. P., GUMKOWSKI, F. D., KONIECZKO, E. M., Fischer von Mollard, G., JAHN, R., and JAMIESON, J. D. (1994). REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS. JOURNAL OF CELL BIOLOGY 124, 43-53.
JENA, B.P., et al., 1994. REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS. JOURNAL OF CELL BIOLOGY, 124(1), p 43-53.
B.P. JENA, et al., “REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS”, JOURNAL OF CELL BIOLOGY, vol. 124, 1994, pp. 43-53.
JENA, B.P., GUMKOWSKI, F.D., KONIECZKO, E.M., Fischer von Mollard, G., JAHN, R., JAMIESON, J.D.: REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS. JOURNAL OF CELL BIOLOGY. 124, 43-53 (1994).
JENA, BP, GUMKOWSKI, FD, KONIECZKO, EM, Fischer von Mollard, Gabriele, JAHN, R, and JAMIESON, JD. “REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS”. JOURNAL OF CELL BIOLOGY 124.1 (1994): 43-53.
This data publication is cited in the following publications:
This publication cites the following data publications:

37 Citations in Europe PMC

Data provided by Europe PubMed Central.

Aquaporin-assisted and ER-mediated mitochondrial fission: a hypothesis.
Lee JS, Hou X, Bishop N, Wang S, Flack A, Cho WJ, Chen X, Mao G, Taatjes DJ, Sun F, Zhang K, Jena BP., Micron 47(), 2013
PMID: 23416165
Rab3D regulates amylase levels, not agonist-induced amylase release, in AR42J cells.
Limi S, Ojakian G, Raffaniello R., Cell. Mol. Biol. Lett. 17(2), 2012
PMID: 22367855
ApRab3, a biosynthetic Rab protein, accumulates on the maturing phagosomes and symbiosomes in the tropical sea anemone, Aiptasia pulchella.
Hong MC, Huang YS, Lin WW, Fang LS, Chen MC., Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 152(3), 2009
PMID: 19110066
Bisperoxovanadium complex promotes dopamine exocytosis in PC12 cells.
Bieger S, Morinville A, Maysinger D., Neurochem. Int. 40(4), 2002
PMID: 11792460
Expression and localization of rab escort protein isoforms in parotid acinar cells from rat.
Chan D, Lin J, Raffaniello RD., J. Cell. Physiol. 185(3), 2000
PMID: 11056004
Secretory vesicle-specific antibodies in the confocal study of exo-endocytosis dynamics.
Slembrouck D, Partoens P, Annaert W, De Potter WP., Methods 18(4), 1999
PMID: 10491276
On the role of rab GTPases: what can be learned from the developing pancreas.
Valentijn JA, Jamieson JD., Biochem. Biophys. Res. Commun. 243(2), 1998
PMID: 9480809
Cloning of novel transcripts of the human guanine-nucleotide-exchange factor Mss4: in situ chromosomal mapping and expression in pancreatic cancer.
Muller-Pillasch F, Zimmerhackl F, Lacher U, Schultz N, Hameister H, Varga G, Friess H, Buchler M, Adler G, Gress TM., Genomics 46(3), 1997
PMID: 9441742
Establishment and immunocharacterization of an immortalized pancreatic cell line derived from the H-2Kb-tsA58 transgenic mouse.
Blouin R, Grondin G, Beaudoin J, Arita Y, Daigle N, Talbot BG, Lebel D, Morisset J., In Vitro Cell. Dev. Biol. Anim. 33(9), 1997
PMID: 9358288
Effect of morphine on secretion of amylase from isolated parotid acini.
Miwa Y, Saeki M, Yamaji A, Maeda S, Saito K., Life Sci. 59(21), 1996
PMID: 8937508

32 References

Data provided by Europe PubMed Central.

GTP-binding proteins in intracellular transport.
Pfeffer SR., Trends Cell Biol. 2(2), 1992
PMID: 14731525
Small GTP-binding proteins.
Takai Y, Kaibuchi K, Kikuchi A, Kawata M., Int. Rev. Cytol. 133(), 1992
PMID: 1577587
Condensation-sorting events in the rough endoplasmic reticulum of exocrine pancreatic cells.
Tooze J, Kern HF, Fuller SD, Howell KE., J. Cell Biol. 109(1), 1989
PMID: 2745555
Mutational analysis of SEC4 suggests a cyclical mechanism for the regulation of vesicular traffic.
Walworth NC, Goud B, Kabcenell AK, Novick PJ., EMBO J. 8(6), 1989
PMID: 2504585

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 8294505
PubMed | Europe PMC

Search this title in

Google Scholar