GTP CLEAVAGE BY THE SMALL GTP-BINDING PROTEIN RAB3A IS ASSOCIATED WITH EXOCYTOSIS OF SYNAPTIC VESICLES INDUCED BY ALPHA-LATROTOXIN

STAHL B, Fischer von Mollard G, WALCHSOLIMENA C, JAHN R (1994)
JOURNAL OF BIOLOGICAL CHEMISTRY 269(40): 24770-24776.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ;
Abstract
Neurotransmitter release from presynaptic nerve terminals is a highly regulated form of exocytosis. Small GTP-binding proteins of the Rab family have been proposed to act as central regulators in this process that cycle between a GTP- and GDP-bound form. Previous work has shown that the synaptic vesicle protein Rab3A undergoes a membrane association-dissociation cycle that is associated with neurotransmitter release. Using isolated nerve terminals as our model system, we have now analyzed the GDP/GTP status of Rab3A. Synaptic vesicle-bound Rab3A was almost exclusively in the GTP form whereas cytosolic Rab3A contained only GDP. Approximately equal amounts of GTP and GDP were found in the pool of Rab3A localized to a membrane fraction containing plasma membrane-synaptic vesicle complexes. In contrast to Rab3A, Rab5 (an endosomal G-protein) was predominantly GDP-bound in all analyzed compartments. To analyze whether Rab3A-bound GTP is cleaved during exocytosis, synaptosomes were stimulated with alpha-latrotoxin, the active component of black widow spider venom. This resulted in massive exocytosis. A significant increase of the GDP/GTP ratio of Rab3A was observed under these conditions that was not due to a nonspecific loss of high energy nucleotides. Our findings suggest that cleavage of Rab3A-bound GTP is a crucial step in regulated exocytosis of synaptic vesicles.
Publishing Year
ISSN
PUB-ID

Cite this

STAHL B, Fischer von Mollard G, WALCHSOLIMENA C, JAHN R. GTP CLEAVAGE BY THE SMALL GTP-BINDING PROTEIN RAB3A IS ASSOCIATED WITH EXOCYTOSIS OF SYNAPTIC VESICLES INDUCED BY ALPHA-LATROTOXIN. JOURNAL OF BIOLOGICAL CHEMISTRY. 1994;269(40):24770-24776.
STAHL, B., Fischer von Mollard, G., WALCHSOLIMENA, C., & JAHN, R. (1994). GTP CLEAVAGE BY THE SMALL GTP-BINDING PROTEIN RAB3A IS ASSOCIATED WITH EXOCYTOSIS OF SYNAPTIC VESICLES INDUCED BY ALPHA-LATROTOXIN. JOURNAL OF BIOLOGICAL CHEMISTRY, 269(40), 24770-24776.
STAHL, B., Fischer von Mollard, G., WALCHSOLIMENA, C., and JAHN, R. (1994). GTP CLEAVAGE BY THE SMALL GTP-BINDING PROTEIN RAB3A IS ASSOCIATED WITH EXOCYTOSIS OF SYNAPTIC VESICLES INDUCED BY ALPHA-LATROTOXIN. JOURNAL OF BIOLOGICAL CHEMISTRY 269, 24770-24776.
STAHL, B., et al., 1994. GTP CLEAVAGE BY THE SMALL GTP-BINDING PROTEIN RAB3A IS ASSOCIATED WITH EXOCYTOSIS OF SYNAPTIC VESICLES INDUCED BY ALPHA-LATROTOXIN. JOURNAL OF BIOLOGICAL CHEMISTRY, 269(40), p 24770-24776.
B. STAHL, et al., “GTP CLEAVAGE BY THE SMALL GTP-BINDING PROTEIN RAB3A IS ASSOCIATED WITH EXOCYTOSIS OF SYNAPTIC VESICLES INDUCED BY ALPHA-LATROTOXIN”, JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 269, 1994, pp. 24770-24776.
STAHL, B., Fischer von Mollard, G., WALCHSOLIMENA, C., JAHN, R.: GTP CLEAVAGE BY THE SMALL GTP-BINDING PROTEIN RAB3A IS ASSOCIATED WITH EXOCYTOSIS OF SYNAPTIC VESICLES INDUCED BY ALPHA-LATROTOXIN. JOURNAL OF BIOLOGICAL CHEMISTRY. 269, 24770-24776 (1994).
STAHL, B, Fischer von Mollard, Gabriele, WALCHSOLIMENA, C, and JAHN, R. “GTP CLEAVAGE BY THE SMALL GTP-BINDING PROTEIN RAB3A IS ASSOCIATED WITH EXOCYTOSIS OF SYNAPTIC VESICLES INDUCED BY ALPHA-LATROTOXIN”. JOURNAL OF BIOLOGICAL CHEMISTRY 269.40 (1994): 24770-24776.
This data publication is cited in the following publications:
This publication cites the following data publications:

Export

0 Marked Publications

Open Data PUB

Search this title in

Google Scholar