THE T-SNARES SYNTAXIN-1 AND SNAP-25 ARE PRESENT ON ORGANELLES THAT PARTICIPATE IN SYNAPTIC VESICLE RECYCLING

WALCHSOLIMENA C, BLASI J, EDELMANN L, CHAPMAN ER, Fischer von Mollard G, JAHN R (1995)
JOURNAL OF CELL BIOLOGY 128(4): 637-645.

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Abstract
Syntaxin 1 and synaptosome-associated protein of 25 kD (SNAP-25) are neuronal plasmalemma proteins that appear to be essential for exocytosis of synaptic vesicles (SVs). Both proteins form a complex with synaptobrevin, an intrinsic membrane protein of SVs. This binding is thought to be responsible for vesicle docking and apparently precedes membrane fusion, According to the current concept, syntaxin 1 and SNAP-25 are members of larger protein families, collectively designated as target-SNAP receptors (t-SNAREs), whose specific localization to subcellular membranes define where transport vesicles bind and fuse, Here we demonstrate that major pools of syntaxin 1 and SNAP-25 recycle with SVs. Both proteins cofractionate with SVs and clathrin-coated vesicles upon subcellular fractionation. Using recombinant proteins as standards for quantitation, we found that syntaxin 1 and SNAP-25 each comprise similar to 3% of the total protein in highly purified SVs. Thus, both proteins are significant components of SVs although less abundant than synaptobrevin (8.7% of the total protein). Immunoisolation of vesicles using synaptophysin and syntaxin specific antibodies revealed that most SVs contain syntaxin 1. The widespread distribution of both syntaxin 1 and SNAP-25 on SVs was further confirmed by immunogold electron microscopy. Botulinum neurotoxin C1, a toxin that blocks exocytosis by proteolyzing syntaxin 1, preferentially cleaves vesicular syntaxin 1. We conclude that t-SNAREs participate in SV recycling in what may be functionally distinct forms.
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WALCHSOLIMENA C, BLASI J, EDELMANN L, CHAPMAN ER, Fischer von Mollard G, JAHN R. THE T-SNARES SYNTAXIN-1 AND SNAP-25 ARE PRESENT ON ORGANELLES THAT PARTICIPATE IN SYNAPTIC VESICLE RECYCLING. JOURNAL OF CELL BIOLOGY. 1995;128(4):637-645.
WALCHSOLIMENA, C., BLASI, J., EDELMANN, L., CHAPMAN, E. R., Fischer von Mollard, G., & JAHN, R. (1995). THE T-SNARES SYNTAXIN-1 AND SNAP-25 ARE PRESENT ON ORGANELLES THAT PARTICIPATE IN SYNAPTIC VESICLE RECYCLING. JOURNAL OF CELL BIOLOGY, 128(4), 637-645.
WALCHSOLIMENA, C., BLASI, J., EDELMANN, L., CHAPMAN, E. R., Fischer von Mollard, G., and JAHN, R. (1995). THE T-SNARES SYNTAXIN-1 AND SNAP-25 ARE PRESENT ON ORGANELLES THAT PARTICIPATE IN SYNAPTIC VESICLE RECYCLING. JOURNAL OF CELL BIOLOGY 128, 637-645.
WALCHSOLIMENA, C., et al., 1995. THE T-SNARES SYNTAXIN-1 AND SNAP-25 ARE PRESENT ON ORGANELLES THAT PARTICIPATE IN SYNAPTIC VESICLE RECYCLING. JOURNAL OF CELL BIOLOGY, 128(4), p 637-645.
C. WALCHSOLIMENA, et al., “THE T-SNARES SYNTAXIN-1 AND SNAP-25 ARE PRESENT ON ORGANELLES THAT PARTICIPATE IN SYNAPTIC VESICLE RECYCLING”, JOURNAL OF CELL BIOLOGY, vol. 128, 1995, pp. 637-645.
WALCHSOLIMENA, C., BLASI, J., EDELMANN, L., CHAPMAN, E.R., Fischer von Mollard, G., JAHN, R.: THE T-SNARES SYNTAXIN-1 AND SNAP-25 ARE PRESENT ON ORGANELLES THAT PARTICIPATE IN SYNAPTIC VESICLE RECYCLING. JOURNAL OF CELL BIOLOGY. 128, 637-645 (1995).
WALCHSOLIMENA, C, BLASI, J, EDELMANN, L, CHAPMAN, ER, Fischer von Mollard, Gabriele, and JAHN, R. “THE T-SNARES SYNTAXIN-1 AND SNAP-25 ARE PRESENT ON ORGANELLES THAT PARTICIPATE IN SYNAPTIC VESICLE RECYCLING”. JOURNAL OF CELL BIOLOGY 128.4 (1995): 637-645.
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