The yeast v-SNARE Vti1p mediates two vesicle transport pathways through interactions with the t-SNAREs Sed5p and Pep12p

Fischer von Mollard G, Nothwehr SF, Stevens TH (1997)
JOURNAL OF CELL BIOLOGY 137(7): 1511-1524.

Journal Article | Published | English

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Abstract
Membrane traffic in eukaryotic cells requires that specific V-SNAREs on transport vesicles interact with specific t-SNAREs on target membranes. We identified a novel Saccharomyces cerevisiae v-SNARE (Vti1p) encoded by the essential gene, VTI1. Vti1p interacts with the prevacuolar t-SNARE Pep12p to direct Golgi to prevacuolar traffic. vti1-1 mutant cells missorted and secreted the soluble vacuolar hydrolase carboxypeptidase Y (CPY) rapidly and reversibly when vti2-1 cells were shifted to the restrictive temperature. However, overexpression of Pep12p suppressed the CPY secretion defect exhibited by vti1-1 cells at 36 degrees C. Characterization of a second vti1 mutant, vti1-11, revealed that Vti1p also plays a role in membrane traffic at a cis-Golgi stage. vri1-11 mutant cells displayed a growth defect and accumulated the ER and early Golgi forms of both CPY and the secreted protein invertase at the nonpermissive temperature. Overexpression of the yeast cis-Golgi t-SNARE Sed5p suppressed the accumulation of the ER form of CPY but did not lead to CPY transport to the vacuole in vti1-11 cells. Overexpression of Sed5p allowed growth in the absence of Vti1p. In vitro binding and coimmunoprecipitation studies revealed that Vti1p interacts directly with the two t-SNAREs, Sed5p and Pep12p. These data suggest that Vti1p plays a role in cis-Golgi membrane traffic, which is essential for yeast viability, and a nonessential role in the fusion of Golgi-derived vesicles with the prevacuolar compartment. Therefore, a single v-SNARE can interact functionally with two different t-SNAREs in directing membrane traffic in yeast.
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Fischer von Mollard G, Nothwehr SF, Stevens TH. The yeast v-SNARE Vti1p mediates two vesicle transport pathways through interactions with the t-SNAREs Sed5p and Pep12p. JOURNAL OF CELL BIOLOGY. 1997;137(7):1511-1524.
Fischer von Mollard, G., Nothwehr, S. F., & Stevens, T. H. (1997). The yeast v-SNARE Vti1p mediates two vesicle transport pathways through interactions with the t-SNAREs Sed5p and Pep12p. JOURNAL OF CELL BIOLOGY, 137(7), 1511-1524.
Fischer von Mollard, G., Nothwehr, S. F., and Stevens, T. H. (1997). The yeast v-SNARE Vti1p mediates two vesicle transport pathways through interactions with the t-SNAREs Sed5p and Pep12p. JOURNAL OF CELL BIOLOGY 137, 1511-1524.
Fischer von Mollard, G., Nothwehr, S.F., & Stevens, T.H., 1997. The yeast v-SNARE Vti1p mediates two vesicle transport pathways through interactions with the t-SNAREs Sed5p and Pep12p. JOURNAL OF CELL BIOLOGY, 137(7), p 1511-1524.
G. Fischer von Mollard, S.F. Nothwehr, and T.H. Stevens, “The yeast v-SNARE Vti1p mediates two vesicle transport pathways through interactions with the t-SNAREs Sed5p and Pep12p”, JOURNAL OF CELL BIOLOGY, vol. 137, 1997, pp. 1511-1524.
Fischer von Mollard, G., Nothwehr, S.F., Stevens, T.H.: The yeast v-SNARE Vti1p mediates two vesicle transport pathways through interactions with the t-SNAREs Sed5p and Pep12p. JOURNAL OF CELL BIOLOGY. 137, 1511-1524 (1997).
Fischer von Mollard, Gabriele, Nothwehr, SF, and Stevens, TH. “The yeast v-SNARE Vti1p mediates two vesicle transport pathways through interactions with the t-SNAREs Sed5p and Pep12p”. JOURNAL OF CELL BIOLOGY 137.7 (1997): 1511-1524.
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