The Saccharomyces cerevisiae v-SNARE Vti1p is required for multiple membrane transport pathways to the vacuole

Fischer von Mollard G, Stevens TH (1999)
MOLECULAR BIOLOGY OF THE CELL 10(6): 1719-1732.

Journal Article | Published | English

No fulltext has been uploaded

Author
Abstract
The interaction between V-SNAREs on transport vesicles and t-SNAREs on target membranes is required for membrane traffic in eukaryotic cells. Here we identify Vti1p as the first V-SNARE protein found to be required for biosynthetic traffic into the yeast vacuole, the equivalent of the mammalian lysosome. Certain vti1-ts yeast mutants are defective in alkaline phosphatase transport from the Golgi to the vacuole and in targeting of aminopeptidase I from the cytosol to the vacuole. VTI1 interacts genetically with the vacuolar t-SNARE VAM3, which is required for transport of both alkaline phosphatase and aminopeptidase I to the vacuole. The V-SNARE Nyv1p forms a SNARE complex with Vam3p in homotypic vacuolar fusion; however, we find that Nyv1p is not required for any of the three biosynthetic pathways to the vacuole. V-SNAREs were thought to ensure specificity in membrane traffic. However, Vti1p also functions in two additional membrane traffic pathways: Vti1p interacts with the t-SNAREs Pep12p in traffic from the TGN to the prevacuolar compartment and with Sed5p in retrograde traffic to the cis-Golgi. The ability of Vti1p to mediate multiple fusion steps requires additional proteins to ensure specificity in membrane traffic.
Publishing Year
ISSN
PUB-ID

Cite this

Fischer von Mollard G, Stevens TH. The Saccharomyces cerevisiae v-SNARE Vti1p is required for multiple membrane transport pathways to the vacuole. MOLECULAR BIOLOGY OF THE CELL. 1999;10(6):1719-1732.
Fischer von Mollard, G., & Stevens, T. H. (1999). The Saccharomyces cerevisiae v-SNARE Vti1p is required for multiple membrane transport pathways to the vacuole. MOLECULAR BIOLOGY OF THE CELL, 10(6), 1719-1732.
Fischer von Mollard, G., and Stevens, T. H. (1999). The Saccharomyces cerevisiae v-SNARE Vti1p is required for multiple membrane transport pathways to the vacuole. MOLECULAR BIOLOGY OF THE CELL 10, 1719-1732.
Fischer von Mollard, G., & Stevens, T.H., 1999. The Saccharomyces cerevisiae v-SNARE Vti1p is required for multiple membrane transport pathways to the vacuole. MOLECULAR BIOLOGY OF THE CELL, 10(6), p 1719-1732.
G. Fischer von Mollard and T.H. Stevens, “The Saccharomyces cerevisiae v-SNARE Vti1p is required for multiple membrane transport pathways to the vacuole”, MOLECULAR BIOLOGY OF THE CELL, vol. 10, 1999, pp. 1719-1732.
Fischer von Mollard, G., Stevens, T.H.: The Saccharomyces cerevisiae v-SNARE Vti1p is required for multiple membrane transport pathways to the vacuole. MOLECULAR BIOLOGY OF THE CELL. 10, 1719-1732 (1999).
Fischer von Mollard, Gabriele, and Stevens, TH. “The Saccharomyces cerevisiae v-SNARE Vti1p is required for multiple membrane transport pathways to the vacuole”. MOLECULAR BIOLOGY OF THE CELL 10.6 (1999): 1719-1732.
This data publication is cited in the following publications:
This publication cites the following data publications:

Export

0 Marked Publications

Open Data PUB

Search this title in

Google Scholar