The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex

Antonin W, Riedel D, Fischer von Mollard G (2000)
JOURNAL OF NEUROSCIENCE 20(15): 5724-5732.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ;
Abstract / Bemerkung
Specific soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins are required for different membrane transport steps. The SNARE Vti1a has been colocalized with Golgi markers and Vti1b with Golgi and the trans-Golgi network or endosomal markers in fibroblast cell lines. Here we study the distribution of Vti1a and Vti1b in brain. Vti1b was found in synaptic vesicles but was not enriched in this organelle. A brain-specific splice variant of Vti1a was identified that had an insertion of seven amino acid residues next to the putative SNARE-interacting helix. This Vti1a-beta was enriched in small synaptic vesicles and clathrin-coated vesicles isolated from nerve terminals. Vti1a-beta also copurified with the synaptic vesicle R-SNARE synaptobrevin during immunoisolation of synaptic vesicles and endosomes. Therefore, both synaptobrevin and Vti1a-beta are integral parts of synaptic vesicles throughout their life cycle. Vti1a-beta was part of a SNARE complex in nerve terminals, which bound N-ethylmaleimide-sensitive factor and alpha-SNAP. This SNARE complex was different from the exocytic SNARE complex because Vti1a-beta was not coimmunoprecipitated with syntaxin 1 or SNAP-25. These data suggest that Vti1a-beta does not function in exocytosis but in a separate SNARE complex in a membrane fusion step during recycling or biogenesis of synaptic vesicles.
Erscheinungsjahr
Zeitschriftentitel
JOURNAL OF NEUROSCIENCE
Band
20
Zeitschriftennummer
15
Seite
5724-5732
ISSN
PUB-ID

Zitieren

Antonin W, Riedel D, Fischer von Mollard G. The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex. JOURNAL OF NEUROSCIENCE. 2000;20(15):5724-5732.
Antonin, W., Riedel, D., & Fischer von Mollard, G. (2000). The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex. JOURNAL OF NEUROSCIENCE, 20(15), 5724-5732.
Antonin, W., Riedel, D., and Fischer von Mollard, G. (2000). The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex. JOURNAL OF NEUROSCIENCE 20, 5724-5732.
Antonin, W., Riedel, D., & Fischer von Mollard, G., 2000. The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex. JOURNAL OF NEUROSCIENCE, 20(15), p 5724-5732.
W. Antonin, D. Riedel, and G. Fischer von Mollard, “The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex”, JOURNAL OF NEUROSCIENCE, vol. 20, 2000, pp. 5724-5732.
Antonin, W., Riedel, D., Fischer von Mollard, G.: The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex. JOURNAL OF NEUROSCIENCE. 20, 5724-5732 (2000).
Antonin, W, Riedel, D, and Fischer von Mollard, Gabriele. “The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex”. JOURNAL OF NEUROSCIENCE 20.15 (2000): 5724-5732.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Suchen in

Google Scholar