A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function

Antonin W, Holroyd C, Fasshauer D, Pabst S, Fischer von Mollard G, Jahn R (2000)
EMBO JOURNAL 19(23): 6453-6464.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Sets of SNARE proteins mediate membrane fusion by assembling into core complexes. Multiple SNAREs are thought to function in different intracellular trafficking steps but it is often unclear which of the SNAREs cooperate in individual fusion reactions. We report that syntaxin 7, syntasin 8, vti1b and endobrevin/ VAMP-8 form a complex that functions in the fusion of late endosomes. Antibodies specific for each protein coprecipitate the complex, inhibit homotypic fusion of late endosomes in vitro and retard delivery of endocytosed epidermal growth factor to lysosomes. The purified proteins form core complexes with biochemical and biophysical properties remarkably similar to the neuronal core complex, although each of the four proteins carries a transmembrane domain and three have independently folded N-terminal domains. Substitution experiments, sequence and structural comparisons revealed that each protein occupies a unique position in the complex, with syntaxin 7 corresponding to syntasin 1, and vti1b and syntaxin 8 corresponding to the N- and C-terminal domains of SNAP-25, respectively. We conclude that the structure of core complexes and their molecular mechanism in membrane fusion is highly conserved between distant SNAREs.
Erscheinungsjahr
Zeitschriftentitel
EMBO JOURNAL
Band
19
Zeitschriftennummer
23
Seite
6453-6464
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Antonin W, Holroyd C, Fasshauer D, Pabst S, Fischer von Mollard G, Jahn R. A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function. EMBO JOURNAL. 2000;19(23):6453-6464.
Antonin, W., Holroyd, C., Fasshauer, D., Pabst, S., Fischer von Mollard, G., & Jahn, R. (2000). A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function. EMBO JOURNAL, 19(23), 6453-6464. doi:10.1093/emboj/19.23.6453
Antonin, W., Holroyd, C., Fasshauer, D., Pabst, S., Fischer von Mollard, G., and Jahn, R. (2000). A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function. EMBO JOURNAL 19, 6453-6464.
Antonin, W., et al., 2000. A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function. EMBO JOURNAL, 19(23), p 6453-6464.
W. Antonin, et al., “A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function”, EMBO JOURNAL, vol. 19, 2000, pp. 6453-6464.
Antonin, W., Holroyd, C., Fasshauer, D., Pabst, S., Fischer von Mollard, G., Jahn, R.: A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function. EMBO JOURNAL. 19, 6453-6464 (2000).
Antonin, W, Holroyd, C, Fasshauer, D, Pabst, S, Fischer von Mollard, Gabriele, and Jahn, R. “A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function”. EMBO JOURNAL 19.23 (2000): 6453-6464.

123 Zitationen in Europe PMC

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D53 is a novel endosomal SNARE-binding protein that enhances interaction of syntaxin 1 with the synaptobrevin 2 complex in vitro.
Proux-Gillardeaux V, Galli T, Callebaut I, Mikhailik A, Calothy G, Marx M., Biochem J 370(pt 1), 2003
PMID: 12376003
Mammalian ykt6 is a neuronal SNARE targeted to a specialized compartment by its profilin-like amino terminal domain.
Hasegawa H, Zinsser S, Rhee Y, Vik-Mo EO, Davanger S, Hay JC., Mol Biol Cell 14(2), 2003
PMID: 12589064
Membrane fusion.
Jahn R, Lang T, Südhof TC., Cell 112(4), 2003
PMID: 12600315
The SNARE motif contributes to rbet1 intracellular targeting and dynamics independently of SNARE interactions.
Joglekar AP, Xu D, Rigotti DJ, Fairman R, Hay JC., J Biol Chem 278(16), 2003
PMID: 12566453
Mycobacterium tuberculosis glycosylated phosphatidylinositol causes phagosome maturation arrest.
Fratti RA, Chua J, Vergne I, Deretic V., Proc Natl Acad Sci U S A 100(9), 2003
PMID: 12702770
A SNARE complex containing SGR3/AtVAM3 and ZIG/VTI11 in gravity-sensing cells is important for Arabidopsis shoot gravitropism.
Yano D, Sato M, Saito C, Sato MH, Morita MT, Tasaka M., Proc Natl Acad Sci U S A 100(14), 2003
PMID: 12815100
Transcytosis: crossing cellular barriers.
Tuma P, Hubbard AL., Physiol Rev 83(3), 2003
PMID: 12843411
The role of mVps18p in clustering, fusion, and intracellular localization of late endocytic organelles.
Poupon V, Stewart A, Gray SR, Piper RC, Luzio JP., Mol Biol Cell 14(10), 2003
PMID: 14517315
Cell vacuolization induced by Helicobacter pylori VacA cytotoxin does not depend on late endosomal SNAREs.
de Bernard M, Moschioni M, Habermann A, Griffiths G, Montecucco C., Cell Microbiol 4(1), 2002
PMID: 11856169
Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform.
Mallard F, Tang BL, Galli T, Tenza D, Saint-Pol A, Yue X, Antony C, Hong W, Goud B, Johannes L., J Cell Biol 156(4), 2002
PMID: 11839770
Bilayered clathrin coats on endosomal vacuoles are involved in protein sorting toward lysosomes.
Sachse M, Urbé S, Oorschot V, Strous GJ, Klumperman J., Mol Biol Cell 13(4), 2002
PMID: 11950941
Membrane fusion in eukaryotic cells.
Mayer A., Annu Rev Cell Dev Biol 18(), 2002
PMID: 12142286
Three-dimensional structure of the amino-terminal domain of syntaxin 6, a SNAP-25 C homolog.
Misura KM, Bock JB, Gonzalez LC, Scheller RH, Weis WI., Proc Natl Acad Sci U S A 99(14), 2002
PMID: 12082176
Insights from yeast endosomes.
Pelham HR., Curr Opin Cell Biol 14(4), 2002
PMID: 12383796
Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules.
Riedel D, Antonin W, Fernandez-Chacon R, Alvarez de Toledo G, Jo T, Geppert M, Valentijn JA, Valentijn K, Jamieson JD, Südhof TC, Jahn R., Mol Cell Biol 22(18), 2002
PMID: 12192047
Three SNARE complexes cooperate to mediate membrane fusion.
Hua Y, Scheller RH., Proc Natl Acad Sci U S A 98(14), 2001
PMID: 11427709
SNARE complex structure and function.
Hay JC., Exp Cell Res 271(1), 2001
PMID: 11697877
The Tlg SNARE complex is required for TGN homotypic fusion.
Brickner JH, Blanchette JM, Sipos G, Fuller RS., J Cell Biol 155(6), 2001
PMID: 11739408

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