EpsinR is an adaptor for the SNARE protein Vti1b

Hirst J, Miller SE, Taylor MJ, Fischer von Mollard G, Robinson MS (2004)
MOLECULAR BIOLOGY OF THE CELL 15(12): 5593-5602.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
EpsinR is a clathrin-coated vesicle (CCV)-associated protein that binds to vti1b, suggesting that it may be a vti1b-selective adaptor. Depletion of epsinR to undetectable levels in HeLa cells using siRNA causes vti1b to redistribute from the perinuclear region to the cell periphery, but vti1a also redistributes in epsinR-depleted cells, and both vti isoforms redistribute in AP-1-depleted cells. As a more direct assay for epsinR function, we isolated CCVs from control and siRNA-treated cells and then looked for differences in cargo content. In clathrin-depleted cells, both coat and cargo proteins are greatly reduced in this preparation. Knocking down epsinR causes a similar to50% reduction in the amount of AP-1 copurifying with CCVs and vice versa, indicating that the two proteins are dependent on each other for maximum incorporation into the coat. In addition, vti1b, but not vti1a, is reduced by >70% in CCVs from both epsinR- and AP-1-depleted cells. Because AP-1 knockdown reduces the amount of epsinR in CCVs, it is possible that its effect on vti1b may be indirect. These findings provide in vivo evidence that epsinR is an adaptor for vti1b, and they also show that CCV isolation can be used as an assay for adaptor function.
Publishing Year
ISSN
PUB-ID

Cite this

Hirst J, Miller SE, Taylor MJ, Fischer von Mollard G, Robinson MS. EpsinR is an adaptor for the SNARE protein Vti1b. MOLECULAR BIOLOGY OF THE CELL. 2004;15(12):5593-5602.
Hirst, J., Miller, S. E., Taylor, M. J., Fischer von Mollard, G., & Robinson, M. S. (2004). EpsinR is an adaptor for the SNARE protein Vti1b. MOLECULAR BIOLOGY OF THE CELL, 15(12), 5593-5602.
Hirst, J., Miller, S. E., Taylor, M. J., Fischer von Mollard, G., and Robinson, M. S. (2004). EpsinR is an adaptor for the SNARE protein Vti1b. MOLECULAR BIOLOGY OF THE CELL 15, 5593-5602.
Hirst, J., et al., 2004. EpsinR is an adaptor for the SNARE protein Vti1b. MOLECULAR BIOLOGY OF THE CELL, 15(12), p 5593-5602.
J. Hirst, et al., “EpsinR is an adaptor for the SNARE protein Vti1b”, MOLECULAR BIOLOGY OF THE CELL, vol. 15, 2004, pp. 5593-5602.
Hirst, J., Miller, S.E., Taylor, M.J., Fischer von Mollard, G., Robinson, M.S.: EpsinR is an adaptor for the SNARE protein Vti1b. MOLECULAR BIOLOGY OF THE CELL. 15, 5593-5602 (2004).
Hirst, J, Miller, SE, Taylor, MJ, Fischer von Mollard, Gabriele, and Robinson, MS. “EpsinR is an adaptor for the SNARE protein Vti1b”. MOLECULAR BIOLOGY OF THE CELL 15.12 (2004): 5593-5602.
This data publication is cited in the following publications:
This publication cites the following data publications:

41 Citations in Europe PMC

Data provided by Europe PubMed Central.

Contributions of epsinR and gadkin to clathrin-mediated intracellular trafficking.
Hirst J, Edgar JR, Borner GH, Li S, Sahlender DA, Antrobus R, Robinson MS., Mol. Biol. Cell 26(17), 2015
PMID: 26179914
Epsin deficiency impairs endocytosis by stalling the actin-dependent invagination of endocytic clathrin-coated pits.
Messa M, Fernandez-Busnadiego R, Sun EW, Chen H, Czapla H, Wrasman K, Wu Y, Ko G, Ross T, Wendland B, De Camilli P., Elife 3(), 2014
PMID: 25122462
From endosomes to the trans-Golgi network.
Lu L, Hong W., Semin. Cell Dev. Biol. 31(), 2014
PMID: 24769370
Cinderella story: PI4P goes from precursor to key signaling molecule.
Tan J, Brill JA., Crit. Rev. Biochem. Mol. Biol. 49(1), 2014
PMID: 24219382
Trafficking of the Menkes copper transporter ATP7A is regulated by clathrin-, AP-2-, AP-1-, and Rab22-dependent steps.
Holloway ZG, Velayos-Baeza A, Howell GJ, Levecque C, Ponnambalam S, Sztul E, Monaco AP., Mol. Biol. Cell 24(11), 2013
PMID: 23596324
Epsin N-terminal homology domains bind on opposite sides of two SNAREs.
Wang J, Gossing M, Fang P, Zimmermann J, Li X, von Mollard GF, Niu L, Teng M., Proc. Natl. Acad. Sci. U.S.A. 108(30), 2011
PMID: 21746902
A comparison of GFP-tagged clathrin light chains with fluorochromated light chains in vivo and in vitro.
Hoffmann A, Dannhauser PN, Groos S, Hinrichsen L, Curth U, Ungewickell EJ., Traffic 11(9), 2010
PMID: 20545906
Coiled-coil interactions are required for post-Golgi R-SNARE trafficking.
Gordon DE, Mirza M, Sahlender DA, Jakovleska J, Peden AA., EMBO Rep. 10(8), 2009
PMID: 19557002
Aftiphilin and gamma-synergin are required for secretagogue sensitivity of Weibel-Palade bodies in endothelial cells.
Lui-Roberts WW, Ferraro F, Nightingale TD, Cutler DF., Mol. Biol. Cell 19(12), 2008
PMID: 18815278
Sorting of the v-SNARE VAMP7 in Dictyostelium discoideum: a role for more than one Adaptor Protein (AP) complex.
Bennett N, Letourneur F, Ragno M, Louwagie M., Exp. Cell Res. 314(15), 2008
PMID: 18634783
The role of cargo proteins in GGA recruitment.
Hirst J, Seaman MN, Buschow SI, Robinson MS., Traffic 8(5), 2007
PMID: 17451558
A role for clathrin in reassembly of the Golgi apparatus.
Radulescu AE, Siddhanta A, Shields D., Mol. Biol. Cell 18(1), 2007
PMID: 17065556
Sorting of major cargo glycoproteins into clathrin-coated vesicles.
Harasaki K, Lubben NB, Harbour M, Taylor MJ, Robinson MS., Traffic 6(11), 2005
PMID: 16190982

19 References

Data provided by Europe PubMed Central.

Signals for sorting of transmembrane proteins to endosomes and lysosomes.
Bonifacino JS, Traub LM., Annu. Rev. Biochem. 72(), 2003
PMID: 12651740
Differential requirements for AP-2 in clathrin-mediated endocytosis.
Conner SD, Schmid SL., J. Cell Biol. 162(5), 2003
PMID: 12952931
Curvature of clathrin-coated pits driven by epsin.
Ford MG, Mills IG, Peter BJ, Vallis Y, Praefcke GJ, Evans PR, McMahon HT., Nature 419(6905), 2002
PMID: 12353027
EpsinR: an ENTH domain-containing protein that interacts with AP-1.
Hirst J, Motley A, Harasaki K, Peak Chew SY, Robinson MS., Mol. Biol. Cell 14(2), 2003
PMID: 12589059
Analysis of clathrin-mediated endocytosis of epidermal growth factor receptor by RNA interference.
Huang F, Khvorova A, Marshall W, Sorkin A., J. Biol. Chem. 279(16), 2004
PMID: 14985334
Clint: a novel clathrin-binding ENTH-domain protein at the Golgi.
Kalthoff C, Groos S, Kohl R, Mahrhold S, Ungewickell EJ., Mol. Biol. Cell 13(11), 2002
PMID: 12429846
Definition of the consensus motif recognized by gamma-adaptin ear domains.
Mattera R, Ritter B, Sidhu SS, McPherson PS, Bonifacino JS., J. Biol. Chem. 279(9), 2004
PMID: 14665628
EpsinR: an AP1/clathrin interacting protein involved in vesicle trafficking.
Mills IG, Praefcke GJ, Vallis Y, Peter BJ, Olesen LE, Gallop JL, Butler PJ, Evans PR, McMahon HT., J. Cell Biol. 160(2), 2003
PMID: 12538641
Clathrin-mediated endocytosis in AP-2-depleted cells.
Motley A, Bright NA, Seaman MN, Robinson MS., J. Cell Biol. 162(5), 2003
PMID: 12952941
Gamma-synergin: an EH domain-containing protein that interacts with gamma-adaptin.
Page LJ, Sowerby PJ, Lui WW, Robinson MS., J. Cell Biol. 146(5), 1999
PMID: 10477754
Clathrin adaptor epsinR is required for retrograde sorting on early endosomal membranes.
Saint-Pol A, Yelamos B, Amessou M, Mills IG, Dugast M, Tenza D, Schu P, Antony C, McMahon HT, Lamaze C, Johannes L., Dev. Cell 6(4), 2004
PMID: 15068792

AUTHOR UNKNOWN, 2001
Phosphatidylinositol 4 phosphate regulates targeting of clathrin adaptor AP-1 complexes to the Golgi.
Wang YJ, Wang J, Sun HQ, Martinez M, Sun YX, Macia E, Kirchhausen T, Albanesi JP, Roth MG, Yin HL., Cell 114(3), 2003
PMID: 12914695
Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics.
Wasiak S, Legendre-Guillemin V, Puertollano R, Blondeau F, Girard M, de Heuvel E, Boismenu D, Bell AW, Bonifacino JS, McPherson PS., J. Cell Biol. 158(5), 2002
PMID: 12213833

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 15371541
PubMed | Europe PMC

Search this title in

Google Scholar