Differential imaging of biological structures with doubly-resonant coherent anti-Stokes Raman scattering (DR-CARS)

Weeks TJ, Huser T (2010)
Journal of Visualized Experiments (JoVE) (44).

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Abstract
A combination of three single wavelength short-pulsed lasers is used to generate coherent anti-Stokes Raman scattering (CARS) and doubly-resonant CARS (DR-CARS). The difference between these signals provides enhanced sensitivity for otherwise difficult to detect coherent Raman signals, enabling imaging of weak Raman scatterers.
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Weeks TJ, Huser T. Differential imaging of biological structures with doubly-resonant coherent anti-Stokes Raman scattering (DR-CARS). Journal of Visualized Experiments (JoVE). 2010;(44).
Weeks, T. J., & Huser, T. (2010). Differential imaging of biological structures with doubly-resonant coherent anti-Stokes Raman scattering (DR-CARS). Journal of Visualized Experiments (JoVE)(44).
Weeks, T. J., and Huser, T. (2010). Differential imaging of biological structures with doubly-resonant coherent anti-Stokes Raman scattering (DR-CARS). Journal of Visualized Experiments (JoVE).
Weeks, T.J., & Huser, T., 2010. Differential imaging of biological structures with doubly-resonant coherent anti-Stokes Raman scattering (DR-CARS). Journal of Visualized Experiments (JoVE), (44).
T.J. Weeks and T. Huser, “Differential imaging of biological structures with doubly-resonant coherent anti-Stokes Raman scattering (DR-CARS)”, Journal of Visualized Experiments (JoVE), 2010.
Weeks, T.J., Huser, T.: Differential imaging of biological structures with doubly-resonant coherent anti-Stokes Raman scattering (DR-CARS). Journal of Visualized Experiments (JoVE). (2010).
Weeks, Tyler J., and Huser, Thomas. “Differential imaging of biological structures with doubly-resonant coherent anti-Stokes Raman scattering (DR-CARS)”. Journal of Visualized Experiments (JoVE) 44 (2010).
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5 References

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Theoretical and experimental characterization of coherent anti-Stokes Raman scattering microscopy
Cheng JX, Volkmer A, Xie XS., 2002
A Review of the Theory and Application of Coherent Anti-Stokes Raman Spectroscopy (CARS)
Tolles WM, Nibler JW, McDonald JR, Harvey AB., 1977
Signal generation and Raman-resonant imaging by non-degenerate four-wave mixing under tight focusing conditions
Weeks T, Schie IW, Wachsmann-Hogiu S, Huser T., 2010
Raman microscopy based on doubly-resonant four-wave mixing (DR-FWM).
Weeks T, Wachsmann-Hogiu S, Huser T., Opt Express 17(19), 2009
PMID: 19770922
Simultaneous forward and epi-CARS microscopy with a single detector by time-correlated single photon counting.
Schie IW, Weeks T, McNerney GP, Fore S, Sampson JK, Wachsmann-Hogiu S, Rutledge JC, Huser T., Opt Express 16(3), 2008
PMID: 18542297

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