Raman spectroscopic analysis of biochemical changes in individual triglyceride-rich lipoproteins in the pre- and postprandial state

Chan JW, Motton D, Rutledge JC, Keim NL, Huser T (2005)
Analytical Chemistry 77(18): 5870-5876.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
Individual triglyceride-rich lipoprotein (TGRL) particles derived from human volunteers are nondestructively analyzed by laser tweezers Raman microspectroscopy, and information on their composition and distribution is obtained. The Raman signature of single optically trapped very low-density lipoproteins (VLDL), a subclass of TGRL, which play an important role in cardiovascular disease, exhibits distinct peaks associated with molecular vibrations of fatty acids, proteins, lipids, and structural rear-rangements of lipids. Our analysis of pre- and postprandial VLDL exhibits the signature of biochemical changes in individual lipoprotein particles following the consumption of meals. Interaction of VLDL with endothelium leads to the breakdown of complex triacylglycerols and the formation of a highly ordered core of free saturated fatty acids in the particle. A particle distribution analysis reveals trends in the degree to which this process has occurred in particles at different times during the postprandial period. Differences in particle distributions based on the different ratios of polyunsaturated to saturated fats in the consumed meals are also easily discerned. Individual lipoprotein particles hydrolyzed in vitro through addition of lipoprotein lipase (LpL) exhibit strikingly similar changes in their Raman spectra. These results demonstrate the feasibility of monitoring the dynamics of lipid metabolism of individual TGRL particles as they interact with LpL in the endothelial cell wall using Raman spectroscopy.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Chan JW, Motton D, Rutledge JC, Keim NL, Huser T. Raman spectroscopic analysis of biochemical changes in individual triglyceride-rich lipoproteins in the pre- and postprandial state. Analytical Chemistry. 2005;77(18):5870-5876.
Chan, J. W., Motton, D., Rutledge, J. C., Keim, N. L., & Huser, T. (2005). Raman spectroscopic analysis of biochemical changes in individual triglyceride-rich lipoproteins in the pre- and postprandial state. Analytical Chemistry, 77(18), 5870-5876.
Chan, J. W., Motton, D., Rutledge, J. C., Keim, N. L., and Huser, T. (2005). Raman spectroscopic analysis of biochemical changes in individual triglyceride-rich lipoproteins in the pre- and postprandial state. Analytical Chemistry 77, 5870-5876.
Chan, J.W., et al., 2005. Raman spectroscopic analysis of biochemical changes in individual triglyceride-rich lipoproteins in the pre- and postprandial state. Analytical Chemistry, 77(18), p 5870-5876.
J.W. Chan, et al., “Raman spectroscopic analysis of biochemical changes in individual triglyceride-rich lipoproteins in the pre- and postprandial state”, Analytical Chemistry, vol. 77, 2005, pp. 5870-5876.
Chan, J.W., Motton, D., Rutledge, J.C., Keim, N.L., Huser, T.: Raman spectroscopic analysis of biochemical changes in individual triglyceride-rich lipoproteins in the pre- and postprandial state. Analytical Chemistry. 77, 5870-5876 (2005).
Chan, J. W., Motton, D., Rutledge, J. C., Keim, N. L., and Huser, Thomas. “Raman spectroscopic analysis of biochemical changes in individual triglyceride-rich lipoproteins in the pre- and postprandial state”. Analytical Chemistry 77.18 (2005): 5870-5876.
This data publication is cited in the following publications:
This publication cites the following data publications:

25 Citations in Europe PMC

Data provided by Europe PubMed Central.

High-resolution optical spectroscopy using multimode interference in a compact tapered fibre.
Wan NH, Meng F, Schroder T, Shiue RJ, Chen EH, Englund D., Nat Commun 6(), 2015
PMID: 26204350
Lipid droplets: a new player in colorectal cancer stem cells unveiled by spectroscopic imaging.
Tirinato L, Liberale C, Di Franco S, Candeloro P, Benfante A, La Rocca R, Potze L, Marotta R, Ruffilli R, Rajamanickam VP, Malerba M, De Angelis F, Falqui A, Carbone E, Todaro M, Medema JP, Stassi G, Di Fabrizio E., Stem Cells 33(1), 2015
PMID: 25186497
Complexity of fatty acid distribution inside human macrophages on single cell level using Raman micro-spectroscopy.
Stiebing C, Matthaus C, Krafft C, Keller AA, Weber K, Lorkowski S, Popp J., Anal Bioanal Chem 406(27), 2014
PMID: 24939132
Inflammasome-mediated secretion of IL-1β in human monocytes through TLR2 activation; modulation by dietary fatty acids.
Snodgrass RG, Huang S, Choi IW, Rutledge JC, Hwang DH., J. Immunol. 191(8), 2013
PMID: 24043885
Direct comparison of fatty acid ratios in single cellular lipid droplets as determined by comparative Raman spectroscopy and gas chromatography.
Schie IW, Nolte L, Pedersen TL, Smith Z, Wu J, Yahiatene I, Newman JW, Huser T., Analyst 138(21), 2013
PMID: 24000336
Optical tweezers for medical diagnostics.
LaFratta CN., Anal Bioanal Chem 405(17), 2013
PMID: 23559336
Postprandial apoE isoform and conformational changes associated with VLDL lipolysis products modulate monocyte inflammation.
den Hartigh LJ, Altman R, Hutchinson R, Petrlova J, Budamagunta MS, Tetali SD, Lagerstedt JO, Voss JC, Rutledge JC., PLoS ONE 7(11), 2012
PMID: 23209766
IRF-1 and miRNA126 modulate VCAM-1 expression in response to a high-fat meal.
Sun C, Alkhoury K, Wang YI, Foster GA, Radecke CE, Tam K, Edwards CM, Facciotti MT, Armstrong EJ, Knowlton AA, Newman JW, Passerini AG, Simon SI., Circ. Res. 111(8), 2012
PMID: 22874466
Lipid-cell interactions in human monocytes investigated by doubly-resonant coherent anti-Stokes Raman scattering microscopy.
Weeks T, Schie I, den Hartigh LJ, Rutledge JC, Huser T., J Biomed Opt 16(2), 2011
PMID: 21361680
Endothelial inflammation correlates with subject triglycerides and waist size after a high-fat meal.
Wang YI, Schulze J, Raymond N, Tomita T, Tam K, Simon SI, Passerini AG., Am. J. Physiol. Heart Circ. Physiol. 300(3), 2011
PMID: 21169396
Confocal Raman microscopy of optical-trapped particles in liquids.
Cherney DP, Harris JM., Annu Rev Anal Chem (Palo Alto Calif) 3(), 2010
PMID: 20636043
Fatty acids from very low-density lipoprotein lipolysis products induce lipid droplet accumulation in human monocytes.
den Hartigh LJ, Connolly-Rohrbach JE, Fore S, Huser TR, Rutledge JC., J. Immunol. 184(7), 2010
PMID: 20208007
Physico-chemical characterization of polylipid nanoparticles for gene delivery to the liver.
Nyunt MT, Dicus CW, Cui YY, Yappert MC, Huser TR, Nantz MH, Wu J., Bioconjug. Chem. 20(11), 2009
PMID: 19860429
FT-IR spectroscopy of lipoproteins--a comparative study.
Krilov D, Balarin M, Kosovic M, Gamulin O, Brnjas-Kraljevic J., Spectrochim Acta A Mol Biomol Spectrosc 73(4), 2009
PMID: 19414281
Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells.
Huser T, Orme CA, Hollars CW, Corzett MH, Balhorn R., J Biophotonics 2(5), 2009
PMID: 19373853
Investigations of thermotropic phase behavior of newly developed synthetic PEGylated lipids using Raman spectro-microscopy.
Bista RK, Bruch RF, Covington AM, Sorger A, Gerstmann T, Otto A., Biopolymers 89(11), 2008
PMID: 18615661
An integrated optofluidic platform for Raman-activated cell sorting.
Lau AY, Lee LP, Chan JW., Lab Chip 8(7), 2008
PMID: 18584087
Monitoring dynamic protein expression in living E. coli. Bacterial cells by laser tweezers Raman spectroscopy.
Chan JW, Winhold H, Corzett MH, Ulloa JM, Cosman M, Balhorn R, Huser T., Cytometry A 71(7), 2007
PMID: 17458881

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 16159116
PubMed | Europe PMC

Search this title in

Google Scholar