Bio-assay based on single molecule fluorescence detection in microfluidic channels

Hollars CW, Puls J, Bakajin O, Olsan B, Talley CE, Lane SM, Huser T (2006)
Analytical and Bioanalytical Chemistry 385(8): 1384-1388.

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Abstract
A rapid bioassay is described based on the detection of colocalized fluorescent DNA probes bound to DNA targets in a pressure-driven solution flowing through a planar microfluidic channel. By employing total internal reflection excitation of the fluorescent probes and illumination of almost the entire flow channel, single fluorescent molecules can be efficiently detected leading to the rapid analysis of nearly the entire solution flowed through the device. Cross-correlation between images obtained from two spectrally distinct probes is used to determine the target concentration and efficiently reduces the number of false positives. The rapid analysis of DNA targets in the low pM range in less than a minute is demonstrated.
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Hollars CW, Puls J, Bakajin O, et al. Bio-assay based on single molecule fluorescence detection in microfluidic channels. Analytical and Bioanalytical Chemistry. 2006;385(8):1384-1388.
Hollars, C. W., Puls, J., Bakajin, O., Olsan, B., Talley, C. E., Lane, S. M., & Huser, T. (2006). Bio-assay based on single molecule fluorescence detection in microfluidic channels. Analytical and Bioanalytical Chemistry, 385(8), 1384-1388.
Hollars, C. W., Puls, J., Bakajin, O., Olsan, B., Talley, C. E., Lane, S. M., and Huser, T. (2006). Bio-assay based on single molecule fluorescence detection in microfluidic channels. Analytical and Bioanalytical Chemistry 385, 1384-1388.
Hollars, C.W., et al., 2006. Bio-assay based on single molecule fluorescence detection in microfluidic channels. Analytical and Bioanalytical Chemistry, 385(8), p 1384-1388.
C.W. Hollars, et al., “Bio-assay based on single molecule fluorescence detection in microfluidic channels”, Analytical and Bioanalytical Chemistry, vol. 385, 2006, pp. 1384-1388.
Hollars, C.W., Puls, J., Bakajin, O., Olsan, B., Talley, C.E., Lane, S.M., Huser, T.: Bio-assay based on single molecule fluorescence detection in microfluidic channels. Analytical and Bioanalytical Chemistry. 385, 1384-1388 (2006).
Hollars, Christopher W., Puls, Jana, Bakajin, Olgica, Olsan, Brad, Talley, Chad E., Lane, Stephen M., and Huser, Thomas. “Bio-assay based on single molecule fluorescence detection in microfluidic channels”. Analytical and Bioanalytical Chemistry 385.8 (2006): 1384-1388.
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