Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence

Ly S, McNerney G, Fore S, Chan J, Huser T (2007)
Optics Express 15(25): 16839-16851.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
We demonstrate time-gated confocal imaging as a means to separate coherent anti-Stokes Raman scattering (CARS) microscopy data from multi-photon excited endogenous fluorescence in tissue. CARS is a quasi-instantaneous process and its signal decay time is only limited by the system's instrument response function (IRF). Signals due to two-photon-excited (TPE) tissue autofluorescence with excited state lifetimes on the nanosecond scale can be identified and separated from the CARS signal by employing time-gating techniques. We demonstrate this improved contrast on the example of CARS microscopy of intact roots of plant seedlings as well as on rat arterial tissue. (c) 2007 Optical Society of America.
Erscheinungsjahr
Zeitschriftentitel
Optics Express
Band
15
Zeitschriftennummer
25
Seite
16839-16851
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Ly S, McNerney G, Fore S, Chan J, Huser T. Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence. Optics Express. 2007;15(25):16839-16851.
Ly, S., McNerney, G., Fore, S., Chan, J., & Huser, T. (2007). Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence. Optics Express, 15(25), 16839-16851. doi:10.1364/OE.15.016839
Ly, S., McNerney, G., Fore, S., Chan, J., and Huser, T. (2007). Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence. Optics Express 15, 16839-16851.
Ly, S., et al., 2007. Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence. Optics Express, 15(25), p 16839-16851.
S. Ly, et al., “Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence”, Optics Express, vol. 15, 2007, pp. 16839-16851.
Ly, S., McNerney, G., Fore, S., Chan, J., Huser, T.: Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence. Optics Express. 15, 16839-16851 (2007).
Ly, Sonny, McNerney, Gregory, Fore, Samantha, Chan, James, and Huser, Thomas. “Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence”. Optics Express 15.25 (2007): 16839-16851.

4 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy.
Slepkov AD, Ridsdale A, Wan HN, Wang MH, Pegoraro AF, Moffatt DJ, Pezacki JP, Kao FJ, Stolow A., J Biomed Opt 16(2), 2011
PMID: 21361666
Photon counting, censor corrections, and lifetime imaging for improved detection in two-photon microscopy.
Driscoll JD, Shih AY, Iyengar S, Field JJ, White GA, Squier JA, Cauwenberghs G, Kleinfeld D., J Neurophysiol 105(6), 2011
PMID: 21471395
Chemical analysis in vivo and in vitro by Raman spectroscopy--from single cells to humans.
Wachsmann-Hogiu S, Weeks T, Huser T., Curr Opin Biotechnol 20(1), 2009
PMID: 19268566
Optical microscopy in photosynthesis.
Cisek R, Spencer L, Prent N, Zigmantas D, Espie GS, Barzda V., Photosynth Res 102(2-3), 2009
PMID: 19851883

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