Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence

Ly S, McNerney G, Fore S, Chan J, Huser T (2007)
Optics Express 15(25): 16839-16851.

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We demonstrate time-gated confocal imaging as a means to separate coherent anti-Stokes Raman scattering (CARS) microscopy data from multi-photon excited endogenous fluorescence in tissue. CARS is a quasi-instantaneous process and its signal decay time is only limited by the system's instrument response function (IRF). Signals due to two-photon-excited (TPE) tissue autofluorescence with excited state lifetimes on the nanosecond scale can be identified and separated from the CARS signal by employing time-gating techniques. We demonstrate this improved contrast on the example of CARS microscopy of intact roots of plant seedlings as well as on rat arterial tissue. (c) 2007 Optical Society of America.
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Ly S, McNerney G, Fore S, Chan J, Huser T. Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence. Optics Express. 2007;15(25):16839-16851.
Ly, S., McNerney, G., Fore, S., Chan, J., & Huser, T. (2007). Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence. Optics Express, 15(25), 16839-16851.
Ly, S., McNerney, G., Fore, S., Chan, J., and Huser, T. (2007). Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence. Optics Express 15, 16839-16851.
Ly, S., et al., 2007. Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence. Optics Express, 15(25), p 16839-16851.
S. Ly, et al., “Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence”, Optics Express, vol. 15, 2007, pp. 16839-16851.
Ly, S., McNerney, G., Fore, S., Chan, J., Huser, T.: Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence. Optics Express. 15, 16839-16851 (2007).
Ly, Sonny, McNerney, Gregory, Fore, Samantha, Chan, James, and Huser, Thomas. “Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence”. Optics Express 15.25 (2007): 16839-16851.
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