Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1

McNerney GP, Huebner W, Chen BK, Huser T (2010)
Journal of Biophotonics 3(4): 216-223.

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Cell-cell interactions through direct contact are very important for cellular communication and coordination especially for immune cells. The human immunodeficiency virus type I (HIV-1) induces immune cell interactions between CD4(+) cells to shuttle between T cells via a virological synapse. A goal to understand the process of cell-cell transmission through virological synapses is to determine the cellular states that allow a chance encounter between cells to become a stable cell-cell adhesion. We demonstrate the use of optical tweezers to manipulate uninfected primary CD4(+) T cells near HIV Gag-iGFP transfected Jurkat T cells to probe the determinants that induce stable adhesion. When combined with fast 4D confocal fluorescence microscopy, optical tweezers can be utilized not only to facilitate cell-cell contact, but also to simultaneously track the formation of a virological synapse, and ultimately to probe the events that precede virus transfer. [GRAPHICS] HIV-1 infected T cell (green) decorated with uninfected primary T cells (red) by manipulating the primary cells with an optical tweezers system.
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McNerney GP, Huebner W, Chen BK, Huser T. Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics. 2010;3(4):216-223.
McNerney, G. P., Huebner, W., Chen, B. K., & Huser, T. (2010). Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics, 3(4), 216-223.
McNerney, G. P., Huebner, W., Chen, B. K., and Huser, T. (2010). Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics 3, 216-223.
McNerney, G.P., et al., 2010. Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics, 3(4), p 216-223.
G.P. McNerney, et al., “Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1”, Journal of Biophotonics, vol. 3, 2010, pp. 216-223.
McNerney, G.P., Huebner, W., Chen, B.K., Huser, T.: Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics. 3, 216-223 (2010).
McNerney, Gregory P., Huebner, Wolfgang, Chen, Benjamin K., and Huser, Thomas. “Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1”. Journal of Biophotonics 3.4 (2010): 216-223.
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4 Citations in Europe PMC

Data provided by Europe PubMed Central.

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38 References

Data provided by Europe PubMed Central.

HIV-1 cell to cell transfer across an Env-induced, actin-dependent synapse.
Jolly C, Kashefi K, Hollinshead M, Sattentau QJ., J. Exp. Med. 199(2), 2004
PMID: 14734528
Retroviral spread by induction of virological synapses.
Jolly C, Sattentau QJ., Traffic 5(9), 2004
PMID: 15296489
Modulation of the immunological synapse: a key to HIV-1 pathogenesis?
Fackler OT, Alcover A, Schwartz O., Nat. Rev. Immunol. 7(4), 2007
PMID: 17380160

Ashkin A., 1970
Observation of a single-beam gradient force optical trap for dielectric particles.
Ashkin A, Dziedzic JM, Bjorkholm JE, Chu S., Opt Lett 11(5), 1986
PMID: 19730608
Nondestructive identification of individual leukemia cells by laser trapping Raman spectroscopy.
Chan JW, Taylor DS, Lane SM, Zwerdling T, Tuscano J, Huser T., Anal. Chem. 80(6), 2008
PMID: 18260656

Ozkan M, Wang M, Ozkan C, Flynn R, Birkbeck A, Esener S., 2003
Review of cell and particle trapping in microfluidic systems.
Nilsson J, Evander M, Hammarstrom B, Laurell T., Anal. Chim. Acta 649(2), 2009
PMID: 19699390
Raman study of mechanically induced oxygenation state transition of red blood cells using optical tweezers.
Rao S, Balint S, Cossins B, Guallar V, Petrov D., Biophys. J. 96(1), 2009
PMID: 18931252

Shi L, Shao B, Chen T, Berns M., 2009

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