Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1

McNerney GP, Huebner W, Chen BK, Huser T (2010)
Journal of Biophotonics 3(4): 216-223.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Cell-cell interactions through direct contact are very important for cellular communication and coordination especially for immune cells. The human immunodeficiency virus type I (HIV-1) induces immune cell interactions between CD4(+) cells to shuttle between T cells via a virological synapse. A goal to understand the process of cell-cell transmission through virological synapses is to determine the cellular states that allow a chance encounter between cells to become a stable cell-cell adhesion. We demonstrate the use of optical tweezers to manipulate uninfected primary CD4(+) T cells near HIV Gag-iGFP transfected Jurkat T cells to probe the determinants that induce stable adhesion. When combined with fast 4D confocal fluorescence microscopy, optical tweezers can be utilized not only to facilitate cell-cell contact, but also to simultaneously track the formation of a virological synapse, and ultimately to probe the events that precede virus transfer. [GRAPHICS] HIV-1 infected T cell (green) decorated with uninfected primary T cells (red) by manipulating the primary cells with an optical tweezers system.
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Zeitschriftentitel
Journal of Biophotonics
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3
Zeitschriftennummer
4
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216-223
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McNerney GP, Huebner W, Chen BK, Huser T. Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics. 2010;3(4):216-223.
McNerney, G. P., Huebner, W., Chen, B. K., & Huser, T. (2010). Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics, 3(4), 216-223. doi:10.1002/jbio.200900102
McNerney, G. P., Huebner, W., Chen, B. K., and Huser, T. (2010). Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics 3, 216-223.
McNerney, G.P., et al., 2010. Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics, 3(4), p 216-223.
G.P. McNerney, et al., “Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1”, Journal of Biophotonics, vol. 3, 2010, pp. 216-223.
McNerney, G.P., Huebner, W., Chen, B.K., Huser, T.: Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics. 3, 216-223 (2010).
McNerney, Gregory P., Huebner, Wolfgang, Chen, Benjamin K., and Huser, Thomas. “Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1”. Journal of Biophotonics 3.4 (2010): 216-223.

6 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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