Dimer formation of organic fluorophores reports on biomolecular dynamics under denaturing conditions

Bollmann S, Löllmann M, Sauer M, Doose S (2011)
Physical Chemistry Chemical Physics 13(28): 12874-12882.

Journal Article | Published | English

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Abstract
Stacking interactions between organic fluorophores can cause formation of non-fluorescent H-dimers. Dimer formation and dissociation of two fluorophores site-specifically incorporated in a biomolecule result in fluorescence intermittency that can report on conformational dynamics. We characterize intramolecular dimerization of two oxazine fluorophores MR121 attached to an unstructured polypeptide. Formation of stable non-fluorescent complexes with nano- to microsecond lifetimes is a prerequisite for analysing the intermittent fluorescence emission by fluorescence correlation spectroscopy and extracting relaxation time constants on nano- to millisecond time scales. Destabilization of the generally very stable homodimers by chemical denaturation reduces the lifetime of H-dimers. We demonstrate that H-dimer formation of an oxazine fluorophore reports on end-to-end contact rates in unstructured glycine-serine polypeptides under denaturing conditions.
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Bollmann S, Löllmann M, Sauer M, Doose S. Dimer formation of organic fluorophores reports on biomolecular dynamics under denaturing conditions. Physical Chemistry Chemical Physics. 2011;13(28):12874-12882.
Bollmann, S., Löllmann, M., Sauer, M., & Doose, S. (2011). Dimer formation of organic fluorophores reports on biomolecular dynamics under denaturing conditions. Physical Chemistry Chemical Physics, 13(28), 12874-12882.
Bollmann, S., Löllmann, M., Sauer, M., and Doose, S. (2011). Dimer formation of organic fluorophores reports on biomolecular dynamics under denaturing conditions. Physical Chemistry Chemical Physics 13, 12874-12882.
Bollmann, S., et al., 2011. Dimer formation of organic fluorophores reports on biomolecular dynamics under denaturing conditions. Physical Chemistry Chemical Physics, 13(28), p 12874-12882.
S. Bollmann, et al., “Dimer formation of organic fluorophores reports on biomolecular dynamics under denaturing conditions”, Physical Chemistry Chemical Physics, vol. 13, 2011, pp. 12874-12882.
Bollmann, S., Löllmann, M., Sauer, M., Doose, S.: Dimer formation of organic fluorophores reports on biomolecular dynamics under denaturing conditions. Physical Chemistry Chemical Physics. 13, 12874-12882 (2011).
Bollmann, Stefan, Löllmann, Marc, Sauer, Markus, and Doose, Soeren. “Dimer formation of organic fluorophores reports on biomolecular dynamics under denaturing conditions”. Physical Chemistry Chemical Physics 13.28 (2011): 12874-12882.
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