Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores

Sischka A, Kleimann C, Hachmann W, Schäfer MM, Seuffert I, Tönsing K, Anselmetti D (2008)
Rev. Sci. Instrum. 79(6).

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Abstract
We introduce a versatile and high precision three-dimensional optical tweezers setup with minimal optical interference to measure small forces and manipulate single molecules in the vicinity of a weak reflective surface. Our tweezers system integrates an inverted optical microscope with a single IR-laser beam that is spatially filtered in an appropriate way to allow force measurements in three dimensions with remarkably high precision when operated in backscattered light detection mode. The setup was tested by overstretching a lambda-DNA in x and z directions (perpendicular and along the optical axis), and by manipulating individual lambda-DNA molecules in the vicinity of a nanopore that allowed quantitative single molecule threading experiments with minimal optical interference. (C) 2008 American Institute of Physics.
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Sischka A, Kleimann C, Hachmann W, et al. Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores. Rev. Sci. Instrum. 2008;79(6).
Sischka, A., Kleimann, C., Hachmann, W., Schäfer, M. M., Seuffert, I., Tönsing, K., & Anselmetti, D. (2008). Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores. Rev. Sci. Instrum., 79(6).
Sischka, A., Kleimann, C., Hachmann, W., Schäfer, M. M., Seuffert, I., Tönsing, K., and Anselmetti, D. (2008). Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores. Rev. Sci. Instrum. 79.
Sischka, A., et al., 2008. Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores. Rev. Sci. Instrum., 79(6).
A. Sischka, et al., “Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores”, Rev. Sci. Instrum., vol. 79, 2008.
Sischka, A., Kleimann, C., Hachmann, W., Schäfer, M.M., Seuffert, I., Tönsing, K., Anselmetti, D.: Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores. Rev. Sci. Instrum. 79, (2008).
Sischka, Andy, Kleimann, Christoph, Hachmann, Wiebke, Schäfer, Markus M., Seuffert, Ina, Tönsing, Katja, and Anselmetti, Dario. “Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores”. Rev. Sci. Instrum. 79.6 (2008).
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9 Citations in Europe PMC

Data provided by Europe PubMed Central.

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