An unexpected link between angiotensinogen and thrombin

Wang Y, Ragg H (2011)
FEBS Letters 585(14): 2395-2399.

Journal Article | Published | English

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Abstract
Angiotensinogen is well known as source protein for a group of potent vasoactive hormones, however, a discrete biochemical activity of the angiotensinogen body is not known. Here we investigated angiotensinogen from the lamprey Lampetra fluviatilis (L. fluviatilis), an early-diverged vertebrate. The recombinantly produced protein showed progressive inhibitory activity towards human alpha-thrombin with a second-order rate constant of 2.6 x 10(4) M(-1) min(-1). Heparin enhanced the reaction rate > 800-fold with a bell-shaped dose-response curve and a stoichiometry of inhibition (SI) of 1.3, revealing lamprey angiotensinogen as an effective a-thrombin inhibitor. Genomic, biochemical, and protein sequence data indicate that angiotensinogen and heparin cofactor II (HCII) originated from a common ancestral thrombin antagonist, thus providing insight into an early stage of thrombin control. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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Wang Y, Ragg H. An unexpected link between angiotensinogen and thrombin. FEBS Letters. 2011;585(14):2395-2399.
Wang, Y., & Ragg, H. (2011). An unexpected link between angiotensinogen and thrombin. FEBS Letters, 585(14), 2395-2399.
Wang, Y., and Ragg, H. (2011). An unexpected link between angiotensinogen and thrombin. FEBS Letters 585, 2395-2399.
Wang, Y., & Ragg, H., 2011. An unexpected link between angiotensinogen and thrombin. FEBS Letters, 585(14), p 2395-2399.
Y. Wang and H. Ragg, “An unexpected link between angiotensinogen and thrombin”, FEBS Letters, vol. 585, 2011, pp. 2395-2399.
Wang, Y., Ragg, H.: An unexpected link between angiotensinogen and thrombin. FEBS Letters. 585, 2395-2399 (2011).
Wang, Yunjie, and Ragg, Hermann. “An unexpected link between angiotensinogen and thrombin”. FEBS Letters 585.14 (2011): 2395-2399.
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