Quantification of Cell Infection Caused by Listeria monocytogenes Invasion

Arif M, Rajpoot N, Technow U, Chakraborty T, Fisch N, Jensen NA, Niehaus K, Nattkemper TW (2011)
Journal of Biotechnology 154(1): 76-83.

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Abstract
Listeria monocytogenes causes a life-threatening food-borne disease known as Listeriosis. Elderly, immunocompromised, and pregnant women are primarily the victims of this facultative intracellular Gram-positive pathogen. Since the bacteria survive intracellularly within the human host cells they are protected against the immune system and poorly accessed by many antibiotics. In order to screen pharmaceutical substances for their ability to interfere with the infection, persistence and release of L. monocytogenes a high content assay is required. We established a high content screen (HCS) using the RAW 264.7 mouse macrophage cell line seeded into 96-well glass bottom microplates. Cells were infected with GFP-expressing L. monocytogenes and stained thereafter with Hoechst 33342. Automated image acquisition was carried out by the ScanR screening station. We have developed an algorithm that automatically grades cells in microscopy images of fluorescent-tagged Listeria for the severity of infection. The grading accuracy of this newly developed algorithm is 97.1% as compared to a 74.3% grading accuracy we obtained using the commercial Olympus ScanR software.
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Arif M, Rajpoot N, Technow U, et al. Quantification of Cell Infection Caused by Listeria monocytogenes Invasion. Journal of Biotechnology. 2011;154(1):76-83.
Arif, M., Rajpoot, N., Technow, U., Chakraborty, T., Fisch, N., Jensen, N. A., Niehaus, K., et al. (2011). Quantification of Cell Infection Caused by Listeria monocytogenes Invasion. Journal of Biotechnology, 154(1), 76-83.
Arif, M., Rajpoot, N., Technow, U., Chakraborty, T., Fisch, N., Jensen, N. A., Niehaus, K., and Nattkemper, T. W. (2011). Quantification of Cell Infection Caused by Listeria monocytogenes Invasion. Journal of Biotechnology 154, 76-83.
Arif, M., et al., 2011. Quantification of Cell Infection Caused by Listeria monocytogenes Invasion. Journal of Biotechnology, 154(1), p 76-83.
M. Arif, et al., “Quantification of Cell Infection Caused by Listeria monocytogenes Invasion”, Journal of Biotechnology, vol. 154, 2011, pp. 76-83.
Arif, M., Rajpoot, N., Technow, U., Chakraborty, T., Fisch, N., Jensen, N.A., Niehaus, K., Nattkemper, T.W.: Quantification of Cell Infection Caused by Listeria monocytogenes Invasion. Journal of Biotechnology. 154, 76-83 (2011).
Arif, Muhammad, Rajpoot, Nasir, Technow, Ulrike, Chakraborty, Trinad, Fisch, Nicole, Jensen, Nickels A., Niehaus, Karsten, and Nattkemper, Tim Wilhelm. “Quantification of Cell Infection Caused by Listeria monocytogenes Invasion”. Journal of Biotechnology 154.1 (2011): 76-83.
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An Emerging Approach for Parallel Quantification of Intracellular Protozoan Parasites and Host Cell Characterization Using TissueFAXS Cytometry.
Schmid M, Dufner B, Durk J, Bedal K, Stricker K, Prokoph LA, Koch C, Wege AK, Zirpel H, van Zandbergen G, Ecker R, Boghiu B, Ritter U., PLoS ONE 10(10), 2015
PMID: 26488169

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