Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite

De Gregoris TB, Rupp O, Klages S, Knaust F, Bekel T, Kube M, Burgess JG, Arnone MI, Goesmann A, Reinhardt R, Clare AS (2011)
Biofouling 27(4): 367-374.

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Abstract
In order to improve the genetic characterisation of the barnacle Balanus amphitrite, normalised EST libraries for the developmental stages, viz. nauplius (a mix of instars I and II), cyprid and adult, were generated. The libraries were sequenced independently using 454 technologies and 575,666 reads were generated. For adults, 4843 unique isotigs were estimated and 6754 and 7506 in the cyprid and naupliar stage, respectively. It was found that some of the previously proposed cyprid-specific bcs genes were also expressed during the naupliar and adult stage. Furthermore, as lectins have been hypothesised to influence settlement cue recognition in barnacles, the database was searched for lectin-like isotigs. Two proteins, uniquely expressed in either the cyprid or the adult stage, matched a mannose receptor, and their nucleotide sequences were 33% and 31% identical to a lectin (BRA-3) isolated from Megabalanus rosa. Further characterisation of these genes may suggest their involvement in settlement.
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De Gregoris TB, Rupp O, Klages S, et al. Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite. Biofouling. 2011;27(4):367-374.
De Gregoris, T. B., Rupp, O., Klages, S., Knaust, F., Bekel, T., Kube, M., Burgess, J. G., et al. (2011). Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite. Biofouling, 27(4), 367-374.
De Gregoris, T. B., Rupp, O., Klages, S., Knaust, F., Bekel, T., Kube, M., Burgess, J. G., Arnone, M. I., Goesmann, A., Reinhardt, R., et al. (2011). Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite. Biofouling 27, 367-374.
De Gregoris, T.B., et al., 2011. Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite. Biofouling, 27(4), p 367-374.
T.B. De Gregoris, et al., “Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite”, Biofouling, vol. 27, 2011, pp. 367-374.
De Gregoris, T.B., Rupp, O., Klages, S., Knaust, F., Bekel, T., Kube, M., Burgess, J.G., Arnone, M.I., Goesmann, A., Reinhardt, R., Clare, A.S.: Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite. Biofouling. 27, 367-374 (2011).
De Gregoris, Tristano Bacchetti, Rupp, Oliver, Klages, Sven, Knaust, Florian, Bekel, Thomas, Kube, Michael, Burgess, J. Grant, Arnone, Maria Ina, Goesmann, Alexander, Reinhardt, Richard, and Clare, Anthony S. “Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite”. Biofouling 27.4 (2011): 367-374.
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