Measuring localization performance of super-resolution algorithms on very active samples

Wolter S, Endesfelder U, van de Linde S, Heilemann M, Sauer M (2011)
Optics Express 19(8): 7020-7033.

Journal Article | Published | English

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Abstract
Super-resolution fluorescence imaging based on single-molecule localization relies critically on the availability of efficient processing algorithms to distinguish, identify, and localize emissions of single fluorophores. In multiple current applications, such as three-dimensional, time-resolved or cluster imaging, high densities of fluorophore emissions are common. Here, we provide an analytic tool to test the performance and quality of localization microscopy algorithms and demonstrate that common algorithms encounter difficulties for samples with high fluorophore density. We demonstrate that, for typical single-molecule localization microscopy methods such as dSTORM and the commonly used rapidSTORM scheme, computational precision limits the acceptable density of concurrently active fluorophores to 0.6 per square micrometer and that the number of successfully localized fluorophores per frame is limited to 0.2 per square micrometer. (C) 2011 Optical Society of America
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Wolter S, Endesfelder U, van de Linde S, Heilemann M, Sauer M. Measuring localization performance of super-resolution algorithms on very active samples. Optics Express. 2011;19(8):7020-7033.
Wolter, S., Endesfelder, U., van de Linde, S., Heilemann, M., & Sauer, M. (2011). Measuring localization performance of super-resolution algorithms on very active samples. Optics Express, 19(8), 7020-7033.
Wolter, S., Endesfelder, U., van de Linde, S., Heilemann, M., and Sauer, M. (2011). Measuring localization performance of super-resolution algorithms on very active samples. Optics Express 19, 7020-7033.
Wolter, S., et al., 2011. Measuring localization performance of super-resolution algorithms on very active samples. Optics Express, 19(8), p 7020-7033.
S. Wolter, et al., “Measuring localization performance of super-resolution algorithms on very active samples”, Optics Express, vol. 19, 2011, pp. 7020-7033.
Wolter, S., Endesfelder, U., van de Linde, S., Heilemann, M., Sauer, M.: Measuring localization performance of super-resolution algorithms on very active samples. Optics Express. 19, 7020-7033 (2011).
Wolter, Steve, Endesfelder, Ulrike, van de Linde, Sebastian, Heilemann, Mike, and Sauer, Markus. “Measuring localization performance of super-resolution algorithms on very active samples”. Optics Express 19.8 (2011): 7020-7033.
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Artifacts in single-molecule localization microscopy.
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Bayesian localization microscopy based on intensity distribution of fluorophores.
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Error bounds for Gauss-Kronrod quadrature formulae
Ehrich, Mathematics of Computation 62(205), 1994

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