The Cellular Energization State Affects Peripheral Stalk Stability of Plant Vacuolar H+-ATPase and Impairs Vacuolar Acidification

Schnitzer D, Seidel T, Sander T, Golldack D, Dietz K-J (2011)
Plant and Cell Physiology 52(5): 946-956.

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The plant vacuolar H+-ATPase takes part in acidifying compartments of the endomembrane system including the secretory pathway and the vacuoles. The structural variability of the V-ATPase complex as well as its presence in different compartments and tissues involves multiple isoforms of V-ATPase subunits. Furthermore, a versatile regulation is essential to allow for organelle- and tissue-specific fine tuning. In this study, results from V-ATPase complex disassembly with a chaotropic reagent, immunodetection and in vivo fluorescence resonance energy transfer (FRET) analyses point to a regulatory mechanism in plants, which depends on energization and involves the stability of the peripheral stalks as well. Lowering of cellular ATP by feeding 2-deoxyglucose resulted in structural alterations within the V-ATPase, as monitored by changes in FRET efficiency between subunits VHA-E and VHA-C. Potassium iodide-mediated disassembly revealed a reduced stability of V-ATPase after 2-deoxyglucose treatment of the cells, but neither the complete V-1-sector nor VHA-C was released from the membrane in response to 2-deoxyglucose treatment, precluding a reversible dissociation mechanism like in yeast. These data suggest the existence of a regulatory mechanism of plant V-ATPase by modification of the peri-pheral stator structure that is linked to the cellular energization state. This mechanism is distinct from reversible dissociation as reported for the yeast V-ATPase, but might represent an evolutionary precursor of reversible dissociation.
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Schnitzer D, Seidel T, Sander T, Golldack D, Dietz K-J. The Cellular Energization State Affects Peripheral Stalk Stability of Plant Vacuolar H+-ATPase and Impairs Vacuolar Acidification. Plant and Cell Physiology. 2011;52(5):946-956.
Schnitzer, D., Seidel, T., Sander, T., Golldack, D., & Dietz, K. - J. (2011). The Cellular Energization State Affects Peripheral Stalk Stability of Plant Vacuolar H+-ATPase and Impairs Vacuolar Acidification. Plant and Cell Physiology, 52(5), 946-956.
Schnitzer, D., Seidel, T., Sander, T., Golldack, D., and Dietz, K. - J. (2011). The Cellular Energization State Affects Peripheral Stalk Stability of Plant Vacuolar H+-ATPase and Impairs Vacuolar Acidification. Plant and Cell Physiology 52, 946-956.
Schnitzer, D., et al., 2011. The Cellular Energization State Affects Peripheral Stalk Stability of Plant Vacuolar H+-ATPase and Impairs Vacuolar Acidification. Plant and Cell Physiology, 52(5), p 946-956.
D. Schnitzer, et al., “The Cellular Energization State Affects Peripheral Stalk Stability of Plant Vacuolar H+-ATPase and Impairs Vacuolar Acidification”, Plant and Cell Physiology, vol. 52, 2011, pp. 946-956.
Schnitzer, D., Seidel, T., Sander, T., Golldack, D., Dietz, K.-J.: The Cellular Energization State Affects Peripheral Stalk Stability of Plant Vacuolar H+-ATPase and Impairs Vacuolar Acidification. Plant and Cell Physiology. 52, 946-956 (2011).
Schnitzer, Daniel, Seidel, Thorsten, Sander, Tim, Golldack, Dortje, and Dietz, Karl-Josef. “The Cellular Energization State Affects Peripheral Stalk Stability of Plant Vacuolar H+-ATPase and Impairs Vacuolar Acidification”. Plant and Cell Physiology 52.5 (2011): 946-956.
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