Size exclusion chromatography-An improved method to harvest Corynebacterium glutamicum cells for the analysis of cytosolic metabolites

Persicke M, Plassmeier J, Neuweger H, Rückert C, Pühler A, Kalinowski J (2011)
Journal of Biotechnology 154(2-3): 171-178.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
The efficient separation of Corynebacterium glutamicum cells from culture medium by size exclusion chromatography (SEC) is presented. Residue analysis demonstrated that this method effectively depletes extracellular compounds. For evaluation, SEC was compared with the common methods cold methanol treatment, fast centrifugation and fast filtration. For this purpose, samples of C. glutamicum cells from fermenter cultures were harvested and subjected to a metabolome analysis. In particular, the wild type strain C. glutamicum ATCC13032 and the lysine production strain C. glutamicum DM1730 were grown in a minimal or in a complex medium. Comparison of metabolite pool sizes after harvesting C. glutamicum cells by the methods mentioned above by gas chromatography coupled to mass spectrometry (GC-MS) revealed that SEC is the most suitable method when intracellular metabolite pools are to be measured during growth in complex media or in the presence of significant amounts of secreted metabolites. In contrast to the other methods tested, the SEC method turned out to be fast and able to remove extracellular compounds almost completely. (C) 2010 Elsevier B.V. All rights reserved.
Erscheinungsjahr
Zeitschriftentitel
Journal of Biotechnology
Band
154
Zeitschriftennummer
2-3
Seite
171-178
ISSN
PUB-ID

Zitieren

Persicke M, Plassmeier J, Neuweger H, Rückert C, Pühler A, Kalinowski J. Size exclusion chromatography-An improved method to harvest Corynebacterium glutamicum cells for the analysis of cytosolic metabolites. Journal of Biotechnology. 2011;154(2-3):171-178.
Persicke, M., Plassmeier, J., Neuweger, H., Rückert, C., Pühler, A., & Kalinowski, J. (2011). Size exclusion chromatography-An improved method to harvest Corynebacterium glutamicum cells for the analysis of cytosolic metabolites. Journal of Biotechnology, 154(2-3), 171-178. doi:10.1016/j.jbiotec.2010.08.016
Persicke, M., Plassmeier, J., Neuweger, H., Rückert, C., Pühler, A., and Kalinowski, J. (2011). Size exclusion chromatography-An improved method to harvest Corynebacterium glutamicum cells for the analysis of cytosolic metabolites. Journal of Biotechnology 154, 171-178.
Persicke, M., et al., 2011. Size exclusion chromatography-An improved method to harvest Corynebacterium glutamicum cells for the analysis of cytosolic metabolites. Journal of Biotechnology, 154(2-3), p 171-178.
M. Persicke, et al., “Size exclusion chromatography-An improved method to harvest Corynebacterium glutamicum cells for the analysis of cytosolic metabolites”, Journal of Biotechnology, vol. 154, 2011, pp. 171-178.
Persicke, M., Plassmeier, J., Neuweger, H., Rückert, C., Pühler, A., Kalinowski, J.: Size exclusion chromatography-An improved method to harvest Corynebacterium glutamicum cells for the analysis of cytosolic metabolites. Journal of Biotechnology. 154, 171-178 (2011).
Persicke, Marcus, Plassmeier, Jens, Neuweger, Heiko, Rückert, Christian, Pühler, Alfred, and Kalinowski, Jörn. “Size exclusion chromatography-An improved method to harvest Corynebacterium glutamicum cells for the analysis of cytosolic metabolites”. Journal of Biotechnology 154.2-3 (2011): 171-178.

2 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Computational tools for the secondary analysis of metabolomics experiments.
Booth SC, Weljie AM, Turner RJ., Comput Struct Biotechnol J 4(), 2013
PMID: 24688685
A novel type of N-acetylglutamate synthase is involved in the first step of arginine biosynthesis in Corynebacterium glutamicum.
Petri K, Walter F, Persicke M, Rückert C, Kalinowski J., BMC Genomics 14(), 2013
PMID: 24138314

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 20817050
PubMed | Europe PMC

Suchen in

Google Scholar