TRANSPARENT TESTA1 interacts with R2R3-MYB factors and affects early and late steps of flavonoid biosynthesis in the endothelium of Arabidopsis thaliana seeds

Appelhagen I, Lu G-H, Huep G, Schmelzer E, Weisshaar B, Sagasser M (2011)
The Plant Journal 67(3): 406-419.

Journal Article | Published | English

No fulltext has been uploaded

Abstract
Summary Wild type seed coats of Arabidopsis thaliana are brown due to the accumulation of proanthocyanidin pigments (PAs). The pigmentation requires activation of phenylpropanoid biosynthesis genes and mutations in some of these genes cause a yellow appearance of seeds, termed transparent testa (tt) phenotype. The TT1 gene encodes a WIP-type zinc finger protein and is expressed in the seed coat endothelium where most of the PAs accumulate in wild type plants. In this study we show that TT1 is not only required for correct expression of PA-specific genes in the seed coat, but also affects CHS, encoding the first enzyme of flavonoid biosynthesis. Many steps of this pathway are controlled by complexes of MYB and BHLH proteins with the WD40 factor TTG1. We demonstrate that TT1 can interact with the R2R3 MYB protein TT2 and that ectopic expression of TT2 can partially restore the lack in PA production in tt1. Reduced seed coat pigmentation was obtained using a TT1 variant lacking nuclear localisation signals. Based on our results we propose that the TT2/TT8/TTG1 regulon may also comprise early genes like CHS and discuss steps to further unravel the regulatory network controlling flavonoid accumulation in endothelium cells during A. thaliana seed development.
Publishing Year
ISSN
PUB-ID

Cite this

Appelhagen I, Lu G-H, Huep G, Schmelzer E, Weisshaar B, Sagasser M. TRANSPARENT TESTA1 interacts with R2R3-MYB factors and affects early and late steps of flavonoid biosynthesis in the endothelium of Arabidopsis thaliana seeds. The Plant Journal. 2011;67(3):406-419.
Appelhagen, I., Lu, G. - H., Huep, G., Schmelzer, E., Weisshaar, B., & Sagasser, M. (2011). TRANSPARENT TESTA1 interacts with R2R3-MYB factors and affects early and late steps of flavonoid biosynthesis in the endothelium of Arabidopsis thaliana seeds. The Plant Journal, 67(3), 406-419.
Appelhagen, I., Lu, G. - H., Huep, G., Schmelzer, E., Weisshaar, B., and Sagasser, M. (2011). TRANSPARENT TESTA1 interacts with R2R3-MYB factors and affects early and late steps of flavonoid biosynthesis in the endothelium of Arabidopsis thaliana seeds. The Plant Journal 67, 406-419.
Appelhagen, I., et al., 2011. TRANSPARENT TESTA1 interacts with R2R3-MYB factors and affects early and late steps of flavonoid biosynthesis in the endothelium of Arabidopsis thaliana seeds. The Plant Journal, 67(3), p 406-419.
I. Appelhagen, et al., “TRANSPARENT TESTA1 interacts with R2R3-MYB factors and affects early and late steps of flavonoid biosynthesis in the endothelium of Arabidopsis thaliana seeds”, The Plant Journal, vol. 67, 2011, pp. 406-419.
Appelhagen, I., Lu, G.-H., Huep, G., Schmelzer, E., Weisshaar, B., Sagasser, M.: TRANSPARENT TESTA1 interacts with R2R3-MYB factors and affects early and late steps of flavonoid biosynthesis in the endothelium of Arabidopsis thaliana seeds. The Plant Journal. 67, 406-419 (2011).
Appelhagen, Ingo, Lu, Gui-Hua, Huep, Gunnar, Schmelzer, Elmon, Weisshaar, Bernd, and Sagasser, Martin. “TRANSPARENT TESTA1 interacts with R2R3-MYB factors and affects early and late steps of flavonoid biosynthesis in the endothelium of Arabidopsis thaliana seeds”. The Plant Journal 67.3 (2011): 406-419.
This data publication is cited in the following publications:
This publication cites the following data publications:

14 Citations in Europe PMC

Data provided by Europe PubMed Central.

Transcriptional control of flavonoid biosynthesis by MYB-bHLH-WDR complexes.
Xu W, Dubos C, Lepiniec L., Trends Plant Sci. 20(3), 2015
PMID: 25577424
Update on transparent testa mutants from Arabidopsis thaliana: characterisation of new alleles from an isogenic collection.
Appelhagen I, Thiedig K, Nordholt N, Schmidt N, Huep G, Sagasser M, Weisshaar B., Planta 240(5), 2014
PMID: 24903359
Predicting transcriptional circuitry underlying seed coat development.
Khan D, Chan A, Millar JL, Girard IJ, Belmonte MF., Plant Sci. 223(), 2014
PMID: 24767124
Comparative proteomics and physiological characterization of Arabidopsis thaliana seedlings in responses to Ochratoxin A.
Wang Y, Hao J, Zhao W, Yang Z, Wu W, Zhang Y, Xu W, Luo Y, Huang K., Plant Mol. Biol. 82(4-5), 2013
PMID: 23625346
Class III peroxidases are activated in proanthocyanidin-deficient Arabidopsis thaliana seeds.
Jia L, Xu W, Li W, Ye N, Liu R, Shi L, Bin Rahman AN, Fan M, Zhang J., Ann. Bot. 111(5), 2013
PMID: 23448691
Phenolic composition analysis and gene expression in developing seeds of yellow- and black-seeded Brassica napus.
Jiang J, Shao Y, Li A, Lu C, Zhang Y, Wang Y., J Integr Plant Biol 55(6), 2013
PMID: 23445079
Regulation of flavonoid biosynthesis involves an unexpected complex transcriptional regulation of TT8 expression, in Arabidopsis.
Xu W, Grain D, Le Gourrierec J, Harscoet E, Berger A, Jauvion V, Scagnelli A, Berger N, Bidzinski P, Kelemen Z, Salsac F, Baudry A, Routaboul JM, Lepiniec L, Dubos C., New Phytol. 198(1), 2013
PMID: 23398515

68 References

Data provided by Europe PubMed Central.

Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY., Nat. Biotechnol. 22(12), 2004
PMID: 15558047
Mutation rate and novel tt mutants of Arabidopsis thaliana induced by carbon ions.
Shikazono N, Yokota Y, Kitamura S, Suzuki C, Watanabe H, Tano S, Tanaka A., Genetics 163(4), 2003
PMID: 12702688
Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis.
Shirley BW, Kubasek WL, Storz G, Bruggemann E, Koornneef M, Ausubel FM, Goodman HM., Plant J. 8(5), 1995
PMID: 8528278
The R2R3-MYB gene family in Arabidopsis thaliana.
Stracke R, Werber M, Weisshaar B., Curr. Opin. Plant Biol. 4(5), 2001
PMID: 11597504
The Arabidopsis bZIP transcription factor HY5 regulates expression of the PFG1/MYB12 gene in response to light and ultraviolet-B radiation.
Stracke R, Favory JJ, Gruber H, Bartelniewoehner L, Bartels S, Binkert M, Funk M, Weisshaar B, Ulm R., Plant Cell Environ. 33(1), 2010
PMID: 19895401
Isolation of total RNA from Arabidopsis thaliana seeds.
Vicient CM, Delseny M., Anal. Biochem. 268(2), 1999
PMID: 10075836
The TRANSPARENT TESTA GLABRA1 locus, which regulates trichome differentiation and anthocyanin biosynthesis in Arabidopsis, encodes a WD40 repeat protein.
Walker AR, Davison PA, Bolognesi-Winfield AC, James CM, Srinivasan N, Blundell TL, Esch JJ, Marks MD, Gray JC., Plant Cell 11(7), 1999
PMID: 10402433
Phenylpropanoid biosynthesis and its regulation.
Weisshaar B, Jenkins GI., Curr. Opin. Plant Biol. 1(3), 1998
PMID: 10066590
Biosynthesis of flavonoids and effects of stress.
Winkel-Shirley B., Curr. Opin. Plant Biol. 5(3), 2002
PMID: 11960739
Knock-out mutants from an En-1 mutagenized Arabidopsis thaliana population generate phenylpropanoid biosynthesis phenotypes.
Wisman E, Hartmann U, Sagasser M, Baumann E, Palme K, Hahlbrock K, Saedler H, Weisshaar B., Proc. Natl. Acad. Sci. U.S.A. 95(21), 1998
PMID: 9770503
Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis.
Xie DY, Sharma SB, Paiva NL, Ferreira D, Dixon RA., Science 299(5605), 2003
PMID: 12532018
The mysteries of proanthocyanidin transport and polymerization.
Zhao J, Pang Y, Dixon RA., Plant Physiol. 153(2), 2010
PMID: 20388668

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 21477081
PubMed | Europe PMC

Search this title in

Google Scholar