Orientation-defined alignment and immobilization of DNA between specific surfaces
Venkatesh A, Herth S, Becker A, Reiss G (2011)
Nanotechnology 22(14): 145301.
Download
No fulltext has been uploaded. References only!
Journal Article
| Original Article
| Published
| English
No fulltext has been uploaded
Author
Department
Abstract
DNA-based single-molecule studies, nanoelectronics and nanocargos require a precise placement of DNA in an orientation-defined manner. Until now, there is a lack of orientation-defined alignment and immobilization of DNA over distances smaller than several micrometers. However, this can be realized by designing bifunctionalized DNA with thiol at one end and (3-aminopropyl) triethoxy silane at the other end, which specifically binds to a gold and SiO2 layer after and during alignment, respectively. The electrode assembly consists of platinum as the electrode material for applying the AC voltage and islands of gold and silicon dioxide fabricated at a distance of about 500-800 nm by electron-beam lithography. The orientation-defined alignment and covalent binding of pUC19 DNA to specific surfaces are carried out in frequency ranges of 50 Hz-1 kHz and 100 kHz-1 MHz and observed after metallization of DNA by palladium ions by field emission scanning electron microscopy (FESEM). The bifunctionalized 890 nm long DNA was effectively aligned and immobilized between a gap of 500 to 600 nm width.
Publishing Year
ISSN
eISSN
PUB-ID
Cite this
Venkatesh A, Herth S, Becker A, Reiss G. Orientation-defined alignment and immobilization of DNA between specific surfaces. Nanotechnology. 2011;22(14):145301.
Venkatesh, A., Herth, S., Becker, A., & Reiss, G. (2011). Orientation-defined alignment and immobilization of DNA between specific surfaces. Nanotechnology, 22(14), 145301. doi:10.1088/0957-4484/22/14/145301
Venkatesh, A., Herth, S., Becker, A., and Reiss, G. (2011). Orientation-defined alignment and immobilization of DNA between specific surfaces. Nanotechnology 22, 145301.
Venkatesh, A., et al., 2011. Orientation-defined alignment and immobilization of DNA between specific surfaces. Nanotechnology, 22(14), p 145301.
A. Venkatesh, et al., “Orientation-defined alignment and immobilization of DNA between specific surfaces”, Nanotechnology, vol. 22, 2011, pp. 145301.
Venkatesh, A., Herth, S., Becker, A., Reiss, G.: Orientation-defined alignment and immobilization of DNA between specific surfaces. Nanotechnology. 22, 145301 (2011).
Venkatesh, Alagarswamy, Herth, Simone, Becker, Anke, and Reiss, Günter. “Orientation-defined alignment and immobilization of DNA between specific surfaces”. Nanotechnology 22.14 (2011): 145301.
This data publication is cited in the following publications:
This publication cites the following data publications:
28 References
Data provided by Europe PubMed Central.
Alignment and sensitive detection of DNA by a moving interface.
Bensimon A, Simon A, Chiffaudel A, Croquette V, Heslot F, Bensimon D., Science 265(5181), 1994
PMID: 7522347
Bensimon A, Simon A, Chiffaudel A, Croquette V, Heslot F, Bensimon D., Science 265(5181), 1994
PMID: 7522347
Precise DNA placement and stretching in electrode gaps using electric fields in a microfluidic system
Dukkipati, Applied Physics Letters 90(8), 2007
Dukkipati, Applied Physics Letters 90(8), 2007
Trapping of 27 bp–8 kbp DNA and immobilization of thiol-modified DNA using dielectrophoresis
Tuukkanen, Nanotechnology 18(29), 2007
Tuukkanen, Nanotechnology 18(29), 2007
Export
0 Marked PublicationsWeb of Science
View record in Web of Science®Sources
PMID: 21346298
PubMed | Europe PMC