Spontaneous degradation of pRD1 DNA into unique size classes is recA dependent

Pühler A, Burkardt HJ, Cannon FC, Wohlleben W (1979)
Mol Gen Genet 171(1): 1-6.

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Abstract
The his and nif genes of the P1 type plasmid pRD1 were lost at a high frequency in a recA+ but not in a recA- Escherichia coli host during growth in a non-selective medium. 92% of the His- Nif- segregants after 6 subcultures retained the genetic markers of the precursor plasmid RP4, while the remainder lost all of the pRD1 markers with the concomitant loss of ccc-DNA. Plasmids purified from the His- Nif- segregants resembled RP4 in the physical and genetic properties examined. The contour length of pRD1 DNA molecules from a recA- strain was 49 micrometer corresponding to a molecular weight of 101 Mdals and the buoyant density was 1.715 g/cm3. In contrast, the contour lengths of plasmid molecules isolated from a recA+ host carrying pRD1 fell into 3 size classes of 49, 19 and 2 micrometer corresponding to molecular weights of 101, 39 and 4 Mdals respectively and two DNA species of buoyant density 1.715 and 1.719 g/cm3 were observed.
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Pühler A, Burkardt HJ, Cannon FC, Wohlleben W. Spontaneous degradation of pRD1 DNA into unique size classes is recA dependent. Mol Gen Genet. 1979;171(1):1-6.
Pühler, A., Burkardt, H. J., Cannon, F. C., & Wohlleben, W. (1979). Spontaneous degradation of pRD1 DNA into unique size classes is recA dependent. Mol Gen Genet, 171(1), 1-6.
Pühler, A., Burkardt, H. J., Cannon, F. C., and Wohlleben, W. (1979). Spontaneous degradation of pRD1 DNA into unique size classes is recA dependent. Mol Gen Genet 171, 1-6.
Pühler, A., et al., 1979. Spontaneous degradation of pRD1 DNA into unique size classes is recA dependent. Mol Gen Genet, 171(1), p 1-6.
A. Pühler, et al., “Spontaneous degradation of pRD1 DNA into unique size classes is recA dependent”, Mol Gen Genet, vol. 171, 1979, pp. 1-6.
Pühler, A., Burkardt, H.J., Cannon, F.C., Wohlleben, W.: Spontaneous degradation of pRD1 DNA into unique size classes is recA dependent. Mol Gen Genet. 171, 1-6 (1979).
Pühler, Alfred, Burkardt, Hans Joachim, Cannon, F. C., and Wohlleben, Wolfgang. “Spontaneous degradation of pRD1 DNA into unique size classes is recA dependent”. Mol Gen Genet 171.1 (1979): 1-6.
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PMID: 375020
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