Relationship of group P1 plasmids revealed by heteroduplex experiments: RP1, RP4, R68 and RK2 are identical

Burkardt HJ, Riess G, Pühler A (1979)
J Gen Microbiol 114(2): 341-348.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The molecular relationships of the IncP1 plasmids RP1, RP4, R68 and RK2 were tested by electron microscopic examination of heteroduplexes. In several hybridization experiments molecules were detected which had a 7.8% portion of incomplete reannealing. This 'heterologous region' could be explained by the typical renaturation behaviour of the transposon Tn1. The identity of the Tn1 transposon present in RP1 and RP4 was proved by heteroduplex experiments with lambda phage DNA containing this transposon. These results indicated that the plasmids RP1 and RP4 are identical. Additional heteroduplex experiments between plasmids R68.45 and RP8 and between R68.45 and RK2 were performed. R68.45, a derivative of R68, has a small DNA insertion and RP8 can be regarded as a large insertion mutant of RP4; both insertions were used as single-stranded hybridization markers. From the hybrid molecules formed, it was deduced that R68 and RK2 are identical with RP1 and RP4 as far as molecular structure is revealed by the technique used.
Erscheinungsjahr
Zeitschriftentitel
J Gen Microbiol
Band
114
Zeitschriftennummer
2
Seite
341-348
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PUB-ID

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Burkardt HJ, Riess G, Pühler A. Relationship of group P1 plasmids revealed by heteroduplex experiments: RP1, RP4, R68 and RK2 are identical. J Gen Microbiol. 1979;114(2):341-348.
Burkardt, H. J., Riess, G., & Pühler, A. (1979). Relationship of group P1 plasmids revealed by heteroduplex experiments: RP1, RP4, R68 and RK2 are identical. J Gen Microbiol, 114(2), 341-348. doi:10.1099/00221287-114-2-341
Burkardt, H. J., Riess, G., and Pühler, A. (1979). Relationship of group P1 plasmids revealed by heteroduplex experiments: RP1, RP4, R68 and RK2 are identical. J Gen Microbiol 114, 341-348.
Burkardt, H.J., Riess, G., & Pühler, A., 1979. Relationship of group P1 plasmids revealed by heteroduplex experiments: RP1, RP4, R68 and RK2 are identical. J Gen Microbiol, 114(2), p 341-348.
H.J. Burkardt, G. Riess, and A. Pühler, “Relationship of group P1 plasmids revealed by heteroduplex experiments: RP1, RP4, R68 and RK2 are identical”, J Gen Microbiol, vol. 114, 1979, pp. 341-348.
Burkardt, H.J., Riess, G., Pühler, A.: Relationship of group P1 plasmids revealed by heteroduplex experiments: RP1, RP4, R68 and RK2 are identical. J Gen Microbiol. 114, 341-348 (1979).
Burkardt, Hans Joachim, Riess, G., and Pühler, Alfred. “Relationship of group P1 plasmids revealed by heteroduplex experiments: RP1, RP4, R68 and RK2 are identical”. J Gen Microbiol 114.2 (1979): 341-348.

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