[Applied gene technology using biological N2-fixation for an example (author's transl)]

Pühler A (1980)
Arzneimittelforschung 30(3a).

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Abstract / Notes
About biological nitrogen fixation most information on the genetic and enzymatic level is known from investigations with Klebsiella pneumoniae. The gene region for N2-fixation (nif) was transferred from K.pneumoniae to the enterobacterium Escherichia coli. After acquisition of nif E.coli is able to fix nitrogen. Plasmid pRD1 was constructed which carries the nif-region. Using genetic engineering techniques the exact location of nif on pRD1 was determined. Subsequently the nif-region comprising 7 operons with 14 known genes was cloned on a multicopy plasmid vector. The use of the Klebsiella-nif-region in biotechnology is discussed. Difficulties arising in experiments designed to construct N2-fixing plants are mentioned.
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Pühler A. [Applied gene technology using biological N2-fixation for an example (author's transl)]. Arzneimittelforschung. 1980;30(3a).
Pühler, A. (1980). [Applied gene technology using biological N2-fixation for an example (author's transl)]. Arzneimittelforschung, 30(3a)
Pühler, A. (1980). [Applied gene technology using biological N2-fixation for an example (author's transl)]. Arzneimittelforschung 30.
Pühler, A., 1980. [Applied gene technology using biological N2-fixation for an example (author's transl)]. Arzneimittelforschung, 30(3a).
A. Pühler, “[Applied gene technology using biological N2-fixation for an example (author's transl)]”, Arzneimittelforschung, vol. 30, 1980.
Pühler, A.: [Applied gene technology using biological N2-fixation for an example (author's transl)]. Arzneimittelforschung. 30, (1980).
Pühler, Alfred. “[Applied gene technology using biological N2-fixation for an example (author's transl)]”. Arzneimittelforschung 30.3a (1980).
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PMID: 6992791
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