Analysis of the parsley chalcone-synthase promoter in response to different light qualities

Merkle T, Frohnmeyer HJ, Schulze-Lefert P, Dangl JL, Hahlbrock K, Schafer E (1994)
Planta 193(2).

Journal Article | Published | English

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Abstract
We examined the chalcone synthase (chs) promoter from parsley [Petroselinum crispum Miller (A.W. Hill)] for the existence of separate promoter elements responsible for transcriptional activation of the chs gene by UV-B and by blue light. A combination of in-vivo foot-printing in parsley cells and light-induced transient expression assays with different chs promoter constructs in parsley protoplasts was used. Dark controls and blue-light-irradiated cells gave identical in-vivo footprints on the chs promoter. Pre-irradiation with blue light prior to a UV-B-light pulse is known to cause a shift in the timing of UV-B-light-induced increase in chs transcription rates. This shift was also manifested on the DNA template, since UV-B-light-induced in-vivo footprints in cells pretreated with blue light were detected earlier than in cells which had been irradiated with a UV-B-light pulse only. Although there was a clear shift in the timing of footprint appearance, the patterns of footprinting did not change. Light-induced transient-expression assays revealed that the shortest tested chs promoter which retained any light responsiveness, was sufficient for mediating both induction by UV light and the blue-light-mediated kinetic shift. These findings argue against a spatial separation of UV-B- and blue-light-responsive elements on the chs promoter. We interpret these data by postulating that the signal transduction pathways originating from the excitation of UV-B- and blue-light receptors merge at the chs promoter, or somewhere between light perception and protein-DNA interaction.
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Merkle T, Frohnmeyer HJ, Schulze-Lefert P, Dangl JL, Hahlbrock K, Schafer E. Analysis of the parsley chalcone-synthase promoter in response to different light qualities. Planta. 1994;193(2).
Merkle, T., Frohnmeyer, H. J., Schulze-Lefert, P., Dangl, J. L., Hahlbrock, K., & Schafer, E. (1994). Analysis of the parsley chalcone-synthase promoter in response to different light qualities. Planta, 193(2).
Merkle, T., Frohnmeyer, H. J., Schulze-Lefert, P., Dangl, J. L., Hahlbrock, K., and Schafer, E. (1994). Analysis of the parsley chalcone-synthase promoter in response to different light qualities. Planta 193.
Merkle, T., et al., 1994. Analysis of the parsley chalcone-synthase promoter in response to different light qualities. Planta, 193(2).
T. Merkle, et al., “Analysis of the parsley chalcone-synthase promoter in response to different light qualities”, Planta, vol. 193, 1994.
Merkle, T., Frohnmeyer, H.J., Schulze-Lefert, P., Dangl, J.L., Hahlbrock, K., Schafer, E.: Analysis of the parsley chalcone-synthase promoter in response to different light qualities. Planta. 193, (1994).
Merkle, Thomas, Frohnmeyer, H. J., Schulze-Lefert, P., Dangl, J. L., Hahlbrock, K., and Schafer, E. “Analysis of the parsley chalcone-synthase promoter in response to different light qualities”. Planta 193.2 (1994).
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PMID: 7764988
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