A plant in vitro system for the nuclear import of proteins

Merkle T, Leclerc D, Marshallsay C, Nagy F (1996)
The Plant Journal 10(6): 1177-1186.

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This paper reports the development of an in vitro system that allows the direct assay of protein import into plant nuclei. In this assay the import of fluorescently labelled karyophilic protein substrates into nuclei isolated from evacuolated tobacco BY-2 suspension cells is monitored. It is demonstrated that import of the fluorescently labelled peptide conjugates is rapid, saturable and nuclear localization signal (NLS)-dependent. Exclusion of high molecular weight (70 kDa) dextran and substrates carrying mutated NLS sequences further underline the specificity of this system. Nuclear translocation of karyophilic import substrates in tobacco, similar to mammalian systems, is inhibited by the non-hydrolysable GTP analogue GTP-gamma-S. In contrast, protein uptake is not blocked by wheat germ agglutinin, N-ethyl-maleinimide and iodoacetic acid. Furthermore, it is shown that nuclear import of proteins is only partially inhibited by low temperature (0-4 degrees C). The in vitro nuclear import assay does not depend on exogenously added ATP or cytosolic factors. However, a block of nuclear import with GTP-gamma-S could be overcome by the addition of cytosolic extract, suggesting the dependence on cytosolic factors or proteins. These data indicate that the characteristics of nuclear protein import in plant and mammalian cells are similar, but may be, at least in some respects, also different from each other.
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Merkle T, Leclerc D, Marshallsay C, Nagy F. A plant in vitro system for the nuclear import of proteins. The Plant Journal. 1996;10(6):1177-1186.
Merkle, T., Leclerc, D., Marshallsay, C., & Nagy, F. (1996). A plant in vitro system for the nuclear import of proteins. The Plant Journal, 10(6), 1177-1186. doi:10.1046/j.1365-313X.1996.10061177.x
Merkle, T., Leclerc, D., Marshallsay, C., and Nagy, F. (1996). A plant in vitro system for the nuclear import of proteins. The Plant Journal 10, 1177-1186.
Merkle, T., et al., 1996. A plant in vitro system for the nuclear import of proteins. The Plant Journal, 10(6), p 1177-1186.
T. Merkle, et al., “A plant in vitro system for the nuclear import of proteins”, The Plant Journal, vol. 10, 1996, pp. 1177-1186.
Merkle, T., Leclerc, D., Marshallsay, C., Nagy, F.: A plant in vitro system for the nuclear import of proteins. The Plant Journal. 10, 1177-1186 (1996).
Merkle, Thomas, Leclerc, D, Marshallsay, C, and Nagy, F. “A plant in vitro system for the nuclear import of proteins”. The Plant Journal 10.6 (1996): 1177-1186.
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Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry.
Berendzen KW, Bohmer M, Wallmeroth N, Peter S, Vesic M, Zhou Y, Tiesler FK, Schleifenbaum F, Harter K., Plant Methods 8(1), 2012
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The Arabidopsis nuclear pore and nuclear envelope.
Meier I, Brkljacic J., Arabidopsis Book 8(), 2010
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Comprehensive prediction of novel microRNA targets in Arabidopsis thaliana.
Alves L Jr, Niemeier S, Hauenschild A, Rehmsmeier M, Merkle T., Nucleic Acids Res. 37(12), 2009
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Nuclear targeting of the cauliflower mosaic virus (CaMV) genome
Champagne J, Leclerc D., Can. J. Bot. 84(4), 2006
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Nuclear import and export of plant virus proteins and genomes.
Krichevsky Alexander, Kozlovsky StanislavV, Gafni Yedidya, Citovsky Vitaly., Mol. Plant Pathol. 7(2), 2006
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Kemen E, Kemen AC, Rafiqi M, Hempel U, Mendgen K, Hahn M, Voegele RT., Mol. Plant Microbe Interact. 18(11), 2005
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Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation.
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Gelvin SB., Annu. Rev. Plant Physiol. Plant Mol. Biol. 51(), 2000
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Nucleic acid transport in plant-microbe interactions: the molecules that walk through the walls.
Tzfira T, Rhee Y, Chen MH, Kunik T, Citovsky V., Annu. Rev. Microbiol. 54(), 2000
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DNA-interactions and nuclear localisation of the chromosomal HMG domain protein SSRP1 from maize.
Rottgers K, Krohn NM, Lichota J, Stemmer C, Merkle T, Grasser KD., Plant J. 23(3), 2000
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Protein nucleocytoplasmic transport and its light regulation in plants.
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Cloning of a cDNA encoding an importin-alpha and down-regulation of the gene by light in rice leaves.
Shoji K, Iwasaki T, Matsuki R, Miyao M, Yamamoto N., Gene 212(2), 1998
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