Rhizobium leguminosarum bv. viciae contains a second fnr/fixK-like gene and an unusual fixL homologue

Patschkowski T, Schlüter A, Priefer UB (1996)
Mol Microbiol 21(2): 267-280.

Journal Article | Published | English

No fulltext has been uploaded

Abstract
Genes of Rhizobium leguminosarum bv. viciae VF39 coding for the regulatory elements NifA, FixL and FixK were isolated, sequenced and genetically analysed. The fixK-fixL region is located upstream of the fixNOQP operon on the non-nodulation plasmid pRleVF39c. The deduced amino acid sequence of FixL revealed an unusual structure in that it contains a receiver module (homologous to the N-terminal domain of response regulators) fused to its transmitter domain. An oxygen-sensing haem-binding domain, found in other FixL proteins, is conserved in R, leguminosarum bv. viciae FixL. R. leguminosarum bv. viciae possesses a second fnr-like gene, designated fixK, whose encoded gene product is very similar to Rhizobium meliloti and Azorhizobium caulinodans FixK. Individual R. leguminosarum bv. viciae fixK and fixL insertion mutants displayed a Fix+ phenotype. A reduced nitrogen-fixation activity was found for a R. leguminosarum bv. viciae fnrN-deletion mutant, whereas no nitrogen-fixation activity was detectable for a flxK/fnrN double mutant. The R. leguminosarum bv. viciae nifA gene is expressed independently of FixL and FixK under aerobic and microaerobic conditions, whereas fixL gene expression is induced under microaerobiosis. Another orf was identified down-stream of fixK-fixL and encodes a product which has homology to pseudoazurins from different species. Mutation of this azu gene showed that it is dispensable for nitrogen fixation.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Patschkowski T, Schlüter A, Priefer UB. Rhizobium leguminosarum bv. viciae contains a second fnr/fixK-like gene and an unusual fixL homologue. Mol Microbiol. 1996;21(2):267-280.
Patschkowski, T., Schlüter, A., & Priefer, U. B. (1996). Rhizobium leguminosarum bv. viciae contains a second fnr/fixK-like gene and an unusual fixL homologue. Mol Microbiol, 21(2), 267-280.
Patschkowski, T., Schlüter, A., and Priefer, U. B. (1996). Rhizobium leguminosarum bv. viciae contains a second fnr/fixK-like gene and an unusual fixL homologue. Mol Microbiol 21, 267-280.
Patschkowski, T., Schlüter, A., & Priefer, U.B., 1996. Rhizobium leguminosarum bv. viciae contains a second fnr/fixK-like gene and an unusual fixL homologue. Mol Microbiol, 21(2), p 267-280.
T. Patschkowski, A. Schlüter, and U.B. Priefer, “Rhizobium leguminosarum bv. viciae contains a second fnr/fixK-like gene and an unusual fixL homologue”, Mol Microbiol, vol. 21, 1996, pp. 267-280.
Patschkowski, T., Schlüter, A., Priefer, U.B.: Rhizobium leguminosarum bv. viciae contains a second fnr/fixK-like gene and an unusual fixL homologue. Mol Microbiol. 21, 267-280 (1996).
Patschkowski, Thomas, Schlüter, Andreas, and Priefer, U. B. “Rhizobium leguminosarum bv. viciae contains a second fnr/fixK-like gene and an unusual fixL homologue”. Mol Microbiol 21.2 (1996): 267-280.
This data publication is cited in the following publications:
This publication cites the following data publications:

22 Citations in Europe PMC

Data provided by Europe PubMed Central.

Transport and metabolism in legume-rhizobia symbioses.
Udvardi M, Poole PS., Annu Rev Plant Biol 64(), 2013
PMID: 23451778
FxkR provides the missing link in the fixL-fixK signal transduction cascade in Rhizobium etli CFN42.
Zamorano-Sanchez D, Reyes-Gonzalez A, Gomez-Hernandez N, Rivera P, Georgellis D, Girard L., Mol. Plant Microbe Interact. 25(11), 2012
PMID: 22809273
Novel reiterated Fnr-type proteins control the production of the symbiotic terminal oxidase cbb3 in Rhizobium etli CFN42.
Granados-Baeza MJ, Gomez-Hernandez N, Mora Y, Delgado MJ, Romero D, Girard L., Mol. Plant Microbe Interact. 20(10), 2007
PMID: 17918626
The genome of Rhizobium leguminosarum has recognizable core and accessory components.
Young JP, Crossman LC, Johnston AW, Thomson NR, Ghazoui ZF, Hull KH, Wexler M, Curson AR, Todd JD, Poole PS, Mauchline TH, East AK, Quail MA, Churcher C, Arrowsmith C, Cherevach I, Chillingworth T, Clarke K, Cronin A, Davis P, Fraser A, Hance Z, Hauser H, Jagels K, Moule S, Mungall K, Norbertczak H, Rabbinowitsch E, Sanders M, Simmonds M, Whitehead S, Parkhill J., Genome Biol. 7(4), 2006
PMID: 16640791
Rhizobium leguminosarum methyl-accepting chemotaxis protein genes are down-regulated in the pea nodule.
Yost CK, Del Bel KL, Quandt J, Hynes MF., Arch. Microbiol. 182(6), 2004
PMID: 15502966
Dicarboxylate transport by rhizobia.
Yurgel SN, Kahn ML., FEMS Microbiol. Rev. 28(4), 2004
PMID: 15374663
Modified RP4 and Tn5-Mob derivatives for facilitated manipulation of large plasmids in Gram-negative bacteria.
Quandt J, Clark RG, Venter AP, Clark SR, Twelker S, Hynes MF., Plasmid 52(1), 2004
PMID: 15212888
Regulatory role of Rhizobium etli CNPAF512 fnrN during symbiosis.
Moris M, Dombrecht B, Xi C, Vanderleyden J, Michiels J., Appl. Environ. Microbiol. 70(3), 2004
PMID: 15006745
Differences in two Pseudomonas aeruginosa cbb3 cytochrome oxidases.
Comolli JC, Donohue TJ., Mol. Microbiol. 51(4), 2004
PMID: 14763990
Conservation of plasmid-encoded traits among bean-nodulating Rhizobium species.
Brom S, Girard L, Garcia-de los Santos A, Sanjuan-Pinilla JM, Olivares J, Sanjuan J., Appl. Environ. Microbiol. 68(5), 2002
PMID: 11976134
Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid.
Barnett MJ, Fisher RF, Jones T, Komp C, Abola AP, Barloy-Hubler F, Bowser L, Capela D, Galibert F, Gouzy J, Gurjal M, Hong A, Huizar L, Hyman RW, Kahn D, Kahn ML, Kalman S, Keating DH, Palm C, Peck MC, Surzycki R, Wells DH, Yeh KC, Davis RW, Federspiel NA, Long SR., Proc. Natl. Acad. Sci. U.S.A. 98(17), 2001
PMID: 11481432
Effect of mutations in Pisum sativum L. genes blocking different stages of nodule development on the expression of late symbiotic genes in Rhizobium leguminosarum bv. viciae.
Voroshilova VA, Boesten B, Tsyganov VE, Borisov AY, Tikhonovich IA, Priefer UB., Mol. Plant Microbe Interact. 14(4), 2001
PMID: 11310734
Symbiotic plasmid rearrangement in Rhizobium leguminosarum bv. viciae VF39SM.
Zhang XX, Kosier B, Priefer UB., J. Bacteriol. 183(6), 2001
PMID: 11222618
Differential regulation of fixN-reiterated genes in Rhizobium etli by a novel fixL-fixK cascade.
Girard L, Brom S, Davalos A, Lopez O, Soberon M, Romero D., Mol. Plant Microbe Interact. 13(12), 2000
PMID: 11106020
Enhanced nitrogen fixation in a rhizobium etli ntrC mutant that overproduces the bradyrhizobium japonicum symbiotic terminal oxidase cbb3
Soberon M, Lopez O, Morera C, Girard ML, Tabche ML, Miranda J., Appl. Environ. Microbiol. 65(5), 1999
PMID: 10223993
PAS domains: internal sensors of oxygen, redox potential, and light.
Taylor BL, Zhulin IB., Microbiol. Mol. Biol. Rev. 63(2), 1999
PMID: 10357859
Bradyrhizobium japonicum FixK2, a crucial distributor in the FixLJ-dependent regulatory cascade for control of genes inducible by low oxygen levels.
Nellen-Anthamatten D, Rossi P, Preisig O, Kullik I, Babst M, Fischer HM, Hennecke H., J. Bacteriol. 180(19), 1998
PMID: 9748464
Expression of the fixR-nifA operon in Bradyrhizobium japonicum depends on a new response regulator, RegR.
Bauer E, Kaspar T, Fischer HM, Hennecke H., J. Bacteriol. 180(15), 1998
PMID: 9683482
Identification of the hydrophobic amino acid residues required for heme assembly in the rhizobial oxygen sensor protein FixL.
Nakamura H, Saito K, Ito E, Tamura K, Tsuchiya T, Nishigaki K, Shiro Y, Iizuka T., Biochem. Biophys. Res. Commun. 247(2), 1998
PMID: 9642144
Expression of thiamin biosynthetic genes (thiCOGE) and production of symbiotic terminal oxidase cbb3 in Rhizobium etli.
Miranda-Rios J, Morera C, Taboada H, Davalos A, Encarnacion S, Mora J, Soberon M., J. Bacteriol. 179(22), 1997
PMID: 9371431
FnrN controls symbiotic nitrogen fixation and hydrogenase activities in Rhizobium leguminosarum biovar viciae UPM791.
Gutierrez D, Hernando Y, Palacios JM, Imperial J, Ruiz-Argueso T., J. Bacteriol. 179(17), 1997
PMID: 9286975
Functional and regulatory analysis of the two copies of the fixNOQP operon of Rhizobium leguminosarum strain VF39.
Schluter A, Patschkowski T, Quandt J, Selinger LB, Weidner S, Kramer M, Zhou L, Hynes MF, Priefer UB., Mol. Plant Microbe Interact. 10(5), 1997
PMID: 9204566

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 8858582
PubMed | Europe PMC

Search this title in

Google Scholar