Competitive and cooperative effects in quorum-sensing-regulated galactoglucan biosynthesis in Sinorhizobium meliloti

McIntosh M, Krol E, Becker A (2008)
Journal of Bacteriology 190(15): 5308-5317.

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The symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti possesses the Sin quorum-sensing system based on N-acyl homoserine lactones (AHLs) as signal molecules. The Sin system consists of SinI, the AHL synthase, and SinR, the LuxR-type regulator. This system regulates the expression of a multitude of S. meliloti genes through ExpR, another LuxR-type regulator. Analysis of the activity of the sinI promoter showed that the expression of sinI is dependent on sinR and enhanced by a combination of expR and Sin AHLs. The characterization of the ExpR binding site upstream of sinI and the identification of binding sites upstream of the galactoglucan biosynthesis genes wgaA (expA1) and wgeA (expE1) allowed the definition of a consensus sequence for these binding sites. Based on this consensus, two additional ExpR binding sites in the promoter regions of exoI and exsH, two genes related to the production of succinoglycan, were found. The specific binding of ExpR to the wgaA and wgeA promoters was enhanced in the presence of oxo-C(14)-HL. Positive regulation of the galactoglucan biosynthesis genes by ExpR was shown to be dependent on WggR (ExpG) and influenced by MucR, both of which are previously characterized regulators of these genes. Based on these results, a reworked model of the Sin-ExpR quorum-sensing regulation scheme of galactoglucan production in S. meliloti is suggested.
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McIntosh M, Krol E, Becker A. Competitive and cooperative effects in quorum-sensing-regulated galactoglucan biosynthesis in Sinorhizobium meliloti. Journal of Bacteriology. 2008;190(15):5308-5317.
McIntosh, M., Krol, E., & Becker, A. (2008). Competitive and cooperative effects in quorum-sensing-regulated galactoglucan biosynthesis in Sinorhizobium meliloti. Journal of Bacteriology, 190(15), 5308-5317. doi:10.1128/JB.00063-08
McIntosh, M., Krol, E., and Becker, A. (2008). Competitive and cooperative effects in quorum-sensing-regulated galactoglucan biosynthesis in Sinorhizobium meliloti. Journal of Bacteriology 190, 5308-5317.
McIntosh, M., Krol, E., & Becker, A., 2008. Competitive and cooperative effects in quorum-sensing-regulated galactoglucan biosynthesis in Sinorhizobium meliloti. Journal of Bacteriology, 190(15), p 5308-5317.
M. McIntosh, E. Krol, and A. Becker, “Competitive and cooperative effects in quorum-sensing-regulated galactoglucan biosynthesis in Sinorhizobium meliloti”, Journal of Bacteriology, vol. 190, 2008, pp. 5308-5317.
McIntosh, M., Krol, E., Becker, A.: Competitive and cooperative effects in quorum-sensing-regulated galactoglucan biosynthesis in Sinorhizobium meliloti. Journal of Bacteriology. 190, 5308-5317 (2008).
McIntosh, Matthew, Krol, Elizaveta, and Becker, Anke. “Competitive and cooperative effects in quorum-sensing-regulated galactoglucan biosynthesis in Sinorhizobium meliloti”. Journal of Bacteriology 190.15 (2008): 5308-5317.
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29 Citations in Europe PMC

Data provided by Europe PubMed Central.

The cin and rai quorum-sensing regulatory systems in Rhizobium leguminosarum are coordinated by ExpR and CinS, a small regulatory protein coexpressed with CinI.
Edwards A, Frederix M, Wisniewski-Dyé F, Jones J, Zorreguieta A, Downie JA., J Bacteriol 191(9), 2009
PMID: 19270098
LuxR-family 'solos': bachelor sensors/regulators of signalling molecules.
Subramoni S, Venturi V., Microbiology 155(pt 5), 2009
PMID: 19383698
Sinorhizobium meliloti regulator MucR couples exopolysaccharide synthesis and motility.
Bahlawane C, McIntosh M, Krol E, Becker A., Mol Plant Microbe Interact 21(11), 2008
PMID: 18842098

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