Positive transcriptional control of the pyridoxal phosphate biosynthesis genes pdxST by the MocR-type regulator PdxR of Corynebacterium glutamicum ATCC 13032

Jochmann N, Götker S, Tauch A (2011)
Microbiology 157(1): 77-88.

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Abstract
The pdxR (cg0897) gene of Corynebacterium glutamicum ATCC 1 3032 encodes a regulatory protein belonging to the MocR subfamily of GntR-type transcription regulators and consisting of an amino-terminal winged helix turn helix DNA-binding domain and a carboxy-terminal aminotransferase-like domain. A defined deletion in the pdxR gene resulted in the decreased expression of the divergently orientated pdxST genes coding for the subunits of pyridoxal 5'-phosphate synthase. The pdxST mutant C. glutamicum NJ0898 and the pdxR mutant C. glutamicum AMH17 showed vitamin B-6 auxotrophy that was restored by supplementing the growth medium with either pyridoxal, pyridoxal 5'-phosphate or pyridoxamine. The genetic organization of the 89 bp pdxR pdxST intergenic region was elucidated by mapping the 5' ends of the respective transcripts, followed by detection of typical promoter sequences. Bioinformatic pattern searches and comparative genomics revealed three DNA motifs with the consensus sequence AAAGTGGW(-/T)CTA, overlapping the deduced promoter sequences and serving as candidate DNA-binding sites for PdxR. DNA band shift assays with the purified PdxR protein demonstrated the specific binding of the transcription regulator to double-stranded 40-mer sequences containing the detected motifs, thereby confirming the direct regulatory role of PdxR in activating the expression of the pdxST genes.
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Jochmann N, Götker S, Tauch A. Positive transcriptional control of the pyridoxal phosphate biosynthesis genes pdxST by the MocR-type regulator PdxR of Corynebacterium glutamicum ATCC 13032. Microbiology. 2011;157(1):77-88.
Jochmann, N., Götker, S., & Tauch, A. (2011). Positive transcriptional control of the pyridoxal phosphate biosynthesis genes pdxST by the MocR-type regulator PdxR of Corynebacterium glutamicum ATCC 13032. Microbiology, 157(1), 77-88. doi:10.1099/mic.0.044818-0
Jochmann, N., Götker, S., and Tauch, A. (2011). Positive transcriptional control of the pyridoxal phosphate biosynthesis genes pdxST by the MocR-type regulator PdxR of Corynebacterium glutamicum ATCC 13032. Microbiology 157, 77-88.
Jochmann, N., Götker, S., & Tauch, A., 2011. Positive transcriptional control of the pyridoxal phosphate biosynthesis genes pdxST by the MocR-type regulator PdxR of Corynebacterium glutamicum ATCC 13032. Microbiology, 157(1), p 77-88.
N. Jochmann, S. Götker, and A. Tauch, “Positive transcriptional control of the pyridoxal phosphate biosynthesis genes pdxST by the MocR-type regulator PdxR of Corynebacterium glutamicum ATCC 13032”, Microbiology, vol. 157, 2011, pp. 77-88.
Jochmann, N., Götker, S., Tauch, A.: Positive transcriptional control of the pyridoxal phosphate biosynthesis genes pdxST by the MocR-type regulator PdxR of Corynebacterium glutamicum ATCC 13032. Microbiology. 157, 77-88 (2011).
Jochmann, Nina, Götker, Susanne, and Tauch, Andreas. “Positive transcriptional control of the pyridoxal phosphate biosynthesis genes pdxST by the MocR-type regulator PdxR of Corynebacterium glutamicum ATCC 13032”. Microbiology 157.1 (2011): 77-88.
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39 References

Data provided by Europe PubMed Central.

Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli.
Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Puhler A., FEMS Microbiol. Lett. 123(3), 1994
PMID: 7988915
Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants.
Grant SG, Jessee J, Bloom FR, Hanahan D., Proc. Natl. Acad. Sci. U.S.A. 87(12), 1990
PMID: 2162051
Structure of a bacterial pyridoxal 5'-phosphate synthase complex.
Strohmeier M, Raschle T, Mazurkiewicz J, Rippe K, Sinning I, Fitzpatrick TB, Tews I., Proc. Natl. Acad. Sci. U.S.A. 103(51), 2006
PMID: 17159152
The transcriptional regulatory network of the amino acid producer Corynebacterium glutamicum.
Brinkrolf K, Brune I, Tauch A., J. Biotechnol. 129(2), 2007
PMID: 17227685
The IclR-type transcriptional repressor LtbR regulates the expression of leucine and tryptophan biosynthesis genes in the amino acid producer Corynebacterium glutamicum.
Brune I, Jochmann N, Brinkrolf K, Huser AT, Gerstmeir R, Eikmanns BJ, Kalinowski J, Puhler A, Tauch A., J. Bacteriol. 189(7), 2007
PMID: 17259312
Clustal W and Clustal X version 2.0.
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG., Bioinformatics 23(21), 2007
PMID: 17846036
The GntR-like regulator TauR activates expression of taurine utilization genes in Rhodobacter capsulatus.
Wiethaus J, Schubert B, Pfander Y, Narberhaus F, Masepohl B., J. Bacteriol. 190(2), 2008
PMID: 17981966
Two independent routes of de novo vitamin B6 biosynthesis: not that different after all.
Fitzpatrick TB, Amrhein N, Kappes B, Macheroux P, Tews I, Raschle T., Biochem. J. 407(1), 2007
PMID: 17822383
CDD: specific functional annotation with the Conserved Domain Database.
Marchler-Bauer A, Anderson JB, Chitsaz F, Derbyshire MK, DeWeese-Scott C, Fong JH, Geer LY, Geer RC, Gonzales NR, Gwadz M, He S, Hurwitz DI, Jackson JD, Ke Z, Lanczycki CJ, Liebert CA, Liu C, Lu F, Lu S, Marchler GH, Mullokandov M, Song JS, Tasneem A, Thanki N, Yamashita RA, Zhang D, Zhang N, Bryant SH., Nucleic Acids Res. 37(Database issue), 2009
PMID: 18984618
Microarray studies reveal a 'differential response' to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum.
Barreiro C, Nakunst D, Huser AT, de Paz HD, Kalinowski J, Martin JF., Microbiology (Reading, Engl.) 155(Pt 2), 2009
PMID: 19202085
Genetic makeup of the Corynebacterium glutamicum LexA regulon deduced from comparative transcriptomics and in vitro DNA band shift assays.
Jochmann N, Kurze AK, Czaja LF, Brinkrolf K, Brune I, Huser AT, Hansmeier N, Puhler A, Borovok I, Tauch A., Microbiology (Reading, Engl.) 155(Pt 5), 2009
PMID: 19372162

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