Protein arginine methylation modulates light-harvesting antenna translation in Chlamydomonas reinhardtii

Blifernez O, Wobbe L, Niehaus K, Kruse O (2011)
The Plant Journal 65(1): 119-130.

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Abstract
P>Methylation of protein arginines represents an important post-translational modification mechanism, which has so far primarily been characterized in mammalian cells. In this work, we successfully identified and characterized arginine methylation as a crucial type of post-translational modification in the activity regulation of the cytosolic translation repressor protein NAB1 in the plant model organism Chlamydomonas reinhardtii. NAB1 represses the cytosolic translation of light-harvesting protein encoding mRNAs by sequestration into translationally silent messenger ribonucleoprotein complexes (mRNPs). Protein arginine methylation of NAB1 could be demonstrated by PRMT1 catalyzed methylation of recombinant NAB1 in vitro, and by immunodetection of methylated NAB1 arginines in vivo. Mass spectrometric analyses of NAB1 purified from C. reinhardtii revealed the asymmetric dimethylation of Arg90 and Arg92 within GAR motif I. Inhibition of arginine methylation by either adenosine-2'-3'-dialdehyde (AdOx) or 7,7'-carbonylbis(azanediyl)bis(4-hydroxynaphthalene-2-sulfonic acid) sodium salt hydrate (AMI-1) caused a dark-green phenotype characterized by the increased accumulation of light-harvesting complex proteins, and indicating a reduced translation repressor activity of NAB1. The extent of NAB1 arginine methylation depends on the growth conditions, with phototrophic growth causing a high methylation state and heterotrophic growth resulting in lowered methylation of the protein. In addition, we could show that NAB1 activity regulation by arginine methylation operates independently from cysteine-based redox control, which has previously been shown to control the activity of NAB1.
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Blifernez O, Wobbe L, Niehaus K, Kruse O. Protein arginine methylation modulates light-harvesting antenna translation in Chlamydomonas reinhardtii. The Plant Journal. 2011;65(1):119-130.
Blifernez, O., Wobbe, L., Niehaus, K., & Kruse, O. (2011). Protein arginine methylation modulates light-harvesting antenna translation in Chlamydomonas reinhardtii. The Plant Journal, 65(1), 119-130.
Blifernez, O., Wobbe, L., Niehaus, K., and Kruse, O. (2011). Protein arginine methylation modulates light-harvesting antenna translation in Chlamydomonas reinhardtii. The Plant Journal 65, 119-130.
Blifernez, O., et al., 2011. Protein arginine methylation modulates light-harvesting antenna translation in Chlamydomonas reinhardtii. The Plant Journal, 65(1), p 119-130.
O. Blifernez, et al., “Protein arginine methylation modulates light-harvesting antenna translation in Chlamydomonas reinhardtii”, The Plant Journal, vol. 65, 2011, pp. 119-130.
Blifernez, O., Wobbe, L., Niehaus, K., Kruse, O.: Protein arginine methylation modulates light-harvesting antenna translation in Chlamydomonas reinhardtii. The Plant Journal. 65, 119-130 (2011).
Blifernez, Olga, Wobbe, Lutz, Niehaus, Karsten, and Kruse, Olaf. “Protein arginine methylation modulates light-harvesting antenna translation in Chlamydomonas reinhardtii”. The Plant Journal 65.1 (2011): 119-130.
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Proteomic Analysis of a Fraction with Intact Eyespots of Chlamydomonas reinhardtii and Assignment of Protein Methylation.
Eitzinger N, Wagner V, Weisheit W, Geimer S, Boness D, Kreimer G, Mittag M., Front Plant Sci 6(), 2015
PMID: 26697039
Integration of carbon assimilation modes with photosynthetic light capture in the green alga Chlamydomonas reinhardtii.
Berger H, Blifernez-Klassen O, Ballottari M, Bassi R, Wobbe L, Kruse O., Mol Plant 7(10), 2014
PMID: 25038233
Regulation and dynamics of the light-harvesting system.
Rochaix JD., Annu Rev Plant Biol 65(), 2014
PMID: 24471838
Construction and evaluation of a whole genome microarray of Chlamydomonas reinhardtii.
Toepel J, Albaum SP, Arvidsson S, Goesmann A, la Russa M, Rogge K, Kruse O., BMC Genomics 12(), 2011
PMID: 22118351

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