Stiffening Effect of Cholesterol on Disordered Lipid Phases: A Combined Neutron Spin Echo plus Dynamic Light Scattering Analysis of the Bending Elasticity of Large Unilamellar Vesicles

Arriaga LR, Lopez-Montero I, Monroy F, Orts-Gil G, Farago B, Hellweg T (2009)
Biophysical Journal 96(9): 3629-3637.

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Abstract
In this study, the center-of-mass diffusion and shape fluctuations of large unilamellar 1-palmitoyl-2-oleyl-sn-glycero-phosphatidylcholine vesicles prepared by extrusion are studied by means of neutron spin echo in combination with dynamic light scattering. The intermediate scattering functions were measured for several different values of the momentum transfer, q, and for different cholesterol contents in the membrane. The combined analysis of neutron spin echo and dynamic light scattering data allows calculation of the bending elastic constant, kappa, of the vesicle bilayer. A stiffening effect monitored as an increase of kappa with increasing cholesterol molar ratio is demonstrated by these measurements.
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Arriaga LR, Lopez-Montero I, Monroy F, Orts-Gil G, Farago B, Hellweg T. Stiffening Effect of Cholesterol on Disordered Lipid Phases: A Combined Neutron Spin Echo plus Dynamic Light Scattering Analysis of the Bending Elasticity of Large Unilamellar Vesicles. Biophysical Journal. 2009;96(9):3629-3637.
Arriaga, L. R., Lopez-Montero, I., Monroy, F., Orts-Gil, G., Farago, B., & Hellweg, T. (2009). Stiffening Effect of Cholesterol on Disordered Lipid Phases: A Combined Neutron Spin Echo plus Dynamic Light Scattering Analysis of the Bending Elasticity of Large Unilamellar Vesicles. Biophysical Journal, 96(9), 3629-3637.
Arriaga, L. R., Lopez-Montero, I., Monroy, F., Orts-Gil, G., Farago, B., and Hellweg, T. (2009). Stiffening Effect of Cholesterol on Disordered Lipid Phases: A Combined Neutron Spin Echo plus Dynamic Light Scattering Analysis of the Bending Elasticity of Large Unilamellar Vesicles. Biophysical Journal 96, 3629-3637.
Arriaga, L.R., et al., 2009. Stiffening Effect of Cholesterol on Disordered Lipid Phases: A Combined Neutron Spin Echo plus Dynamic Light Scattering Analysis of the Bending Elasticity of Large Unilamellar Vesicles. Biophysical Journal, 96(9), p 3629-3637.
L.R. Arriaga, et al., “Stiffening Effect of Cholesterol on Disordered Lipid Phases: A Combined Neutron Spin Echo plus Dynamic Light Scattering Analysis of the Bending Elasticity of Large Unilamellar Vesicles”, Biophysical Journal, vol. 96, 2009, pp. 3629-3637.
Arriaga, L.R., Lopez-Montero, I., Monroy, F., Orts-Gil, G., Farago, B., Hellweg, T.: Stiffening Effect of Cholesterol on Disordered Lipid Phases: A Combined Neutron Spin Echo plus Dynamic Light Scattering Analysis of the Bending Elasticity of Large Unilamellar Vesicles. Biophysical Journal. 96, 3629-3637 (2009).
Arriaga, Laura R., Lopez-Montero, Ivan, Monroy, Francisco, Orts-Gil, Guillermo, Farago, Bela, and Hellweg, Thomas. “Stiffening Effect of Cholesterol on Disordered Lipid Phases: A Combined Neutron Spin Echo plus Dynamic Light Scattering Analysis of the Bending Elasticity of Large Unilamellar Vesicles”. Biophysical Journal 96.9 (2009): 3629-3637.
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