Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein

Vetter G, Hily J-M, Klein E, Schmidlin L, Haas M, Merkle T, Gilmer D (2004)
The Journal of General Virology 85(8): 2459-2469.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ;
Abstract
The protein p25 encoded by beet necrotic yellow vein virus (BNYVV) RNA-3 is involved in symptom expression of infected plants. Confocal microscopy analysis of wild-type and mutated p25 fused to GFP and transiently expressed in BY-2 tobacco suspension cells identified a nuclear localization signal (NLS) in the N-terminal part of the protein. Functionality of the NLS was confirmed by pull-down assays using rice and pepper importin-alpha. Furthermore, it was demonstrated that p25 contains a nuclear export sequence sensitive to leptomycin B. The nuclear export signal (NES) was characterized by mutagenesis. A GFP-p25 fusion protein expressed during a BNYVV infection of Chenopodium quinoa leaves had the same subcellular localization as observed during transient expression in BY-2 cells. The symptom phenotype induced by expression of GFP-p25 during infection was similar to that induced by wild-type virus. Studies with mutated derivatives of GFP-p25 revealed that symptom phenotype was altered when the subcellular localization of GFP-p25 was modified.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Vetter G, Hily J-M, Klein E, et al. Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology. 2004;85(8):2459-2469.
Vetter, G., Hily, J. - M., Klein, E., Schmidlin, L., Haas, M., Merkle, T., & Gilmer, D. (2004). Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology, 85(8), 2459-2469. doi:10.1099/vir.0.80142-0
Vetter, G., Hily, J. - M., Klein, E., Schmidlin, L., Haas, M., Merkle, T., and Gilmer, D. (2004). Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology 85, 2459-2469.
Vetter, G., et al., 2004. Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology, 85(8), p 2459-2469.
G. Vetter, et al., “Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein”, The Journal of General Virology, vol. 85, 2004, pp. 2459-2469.
Vetter, G., Hily, J.-M., Klein, E., Schmidlin, L., Haas, M., Merkle, T., Gilmer, D.: Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology. 85, 2459-2469 (2004).
Vetter, Guillaume, Hily, Jean-Michel, Klein, Elodie, Schmidlin, Laure, Haas, Muriel, Merkle, Thomas, and Gilmer, David. “Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein”. The Journal of General Virology 85.8 (2004): 2459-2469.
This data publication is cited in the following publications:
This publication cites the following data publications:

27 Citations in Europe PMC

Data provided by Europe PubMed Central.

Tricholoma vaccinum host communication during ectomycorrhiza formation.
Wagner K, Linde J, Krause K, Gube M, Koestler T, Sammer D, Kniemeyer O, Kothe E., FEMS Microbiol. Ecol. 91(11), 2015
PMID: 26449385
On the interaction and localization of the beet necrotic yellow vein virus replicase.
Pakdel A, Mounier C, Klein E, Hleibieh K, Monsion B, Mutterer J, Erhardt M, Bouzoubaa S, Ratti C, Gilmer D., Virus Res. 196(), 2015
PMID: 25445349

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 15269388
PubMed | Europe PMC

Search this title in

Google Scholar