Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein

Vetter G, Hily J-M, Klein E, Schmidlin L, Haas M, Merkle T, Gilmer D (2004)
The Journal of General Virology 85(8): 2459-2469.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The protein p25 encoded by beet necrotic yellow vein virus (BNYVV) RNA-3 is involved in symptom expression of infected plants. Confocal microscopy analysis of wild-type and mutated p25 fused to GFP and transiently expressed in BY-2 tobacco suspension cells identified a nuclear localization signal (NLS) in the N-terminal part of the protein. Functionality of the NLS was confirmed by pull-down assays using rice and pepper importin-alpha. Furthermore, it was demonstrated that p25 contains a nuclear export sequence sensitive to leptomycin B. The nuclear export signal (NES) was characterized by mutagenesis. A GFP-p25 fusion protein expressed during a BNYVV infection of Chenopodium quinoa leaves had the same subcellular localization as observed during transient expression in BY-2 cells. The symptom phenotype induced by expression of GFP-p25 during infection was similar to that induced by wild-type virus. Studies with mutated derivatives of GFP-p25 revealed that symptom phenotype was altered when the subcellular localization of GFP-p25 was modified.
Erscheinungsjahr
Zeitschriftentitel
The Journal of General Virology
Band
85
Zeitschriftennummer
8
Seite
2459-2469
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eISSN
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Vetter G, Hily J-M, Klein E, et al. Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology. 2004;85(8):2459-2469.
Vetter, G., Hily, J. - M., Klein, E., Schmidlin, L., Haas, M., Merkle, T., & Gilmer, D. (2004). Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology, 85(8), 2459-2469. doi:10.1099/vir.0.80142-0
Vetter, G., Hily, J. - M., Klein, E., Schmidlin, L., Haas, M., Merkle, T., and Gilmer, D. (2004). Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology 85, 2459-2469.
Vetter, G., et al., 2004. Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology, 85(8), p 2459-2469.
G. Vetter, et al., “Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein”, The Journal of General Virology, vol. 85, 2004, pp. 2459-2469.
Vetter, G., Hily, J.-M., Klein, E., Schmidlin, L., Haas, M., Merkle, T., Gilmer, D.: Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology. 85, 2459-2469 (2004).
Vetter, Guillaume, Hily, Jean-Michel, Klein, Elodie, Schmidlin, Laure, Haas, Muriel, Merkle, Thomas, and Gilmer, David. “Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein”. The Journal of General Virology 85.8 (2004): 2459-2469.

27 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Nuclear import and export of plant virus proteins and genomes.
Krichevsky Alexander, Kozlovsky StanislavV, Gafni Yedidya, Citovsky Vitaly., Mol Plant Pathol 7(2), 2006
PMID: IND43783878
NtLRP1, a tobacco leucine-rich repeat gene with a possible role as a modulator of the hypersensitive response.
Jacques A, Ghannam A, Erhardt M, de Ruffray P, Baillieul F, Kauffmann S., Mol Plant Microbe Interact 19(7), 2006
PMID: 16838787

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