Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein

Vetter G, Hily J-M, Klein E, Schmidlin L, Haas M, Merkle T, Gilmer D (2004)
The Journal of General Virology 85(8): 2459-2469.

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Abstract
The protein p25 encoded by beet necrotic yellow vein virus (BNYVV) RNA-3 is involved in symptom expression of infected plants. Confocal microscopy analysis of wild-type and mutated p25 fused to GFP and transiently expressed in BY-2 tobacco suspension cells identified a nuclear localization signal (NLS) in the N-terminal part of the protein. Functionality of the NLS was confirmed by pull-down assays using rice and pepper importin-alpha. Furthermore, it was demonstrated that p25 contains a nuclear export sequence sensitive to leptomycin B. The nuclear export signal (NES) was characterized by mutagenesis. A GFP-p25 fusion protein expressed during a BNYVV infection of Chenopodium quinoa leaves had the same subcellular localization as observed during transient expression in BY-2 cells. The symptom phenotype induced by expression of GFP-p25 during infection was similar to that induced by wild-type virus. Studies with mutated derivatives of GFP-p25 revealed that symptom phenotype was altered when the subcellular localization of GFP-p25 was modified.
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Vetter G, Hily J-M, Klein E, et al. Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology. 2004;85(8):2459-2469.
Vetter, G., Hily, J. - M., Klein, E., Schmidlin, L., Haas, M., Merkle, T., & Gilmer, D. (2004). Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology, 85(8), 2459-2469.
Vetter, G., Hily, J. - M., Klein, E., Schmidlin, L., Haas, M., Merkle, T., and Gilmer, D. (2004). Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology 85, 2459-2469.
Vetter, G., et al., 2004. Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology, 85(8), p 2459-2469.
G. Vetter, et al., “Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein”, The Journal of General Virology, vol. 85, 2004, pp. 2459-2469.
Vetter, G., Hily, J.-M., Klein, E., Schmidlin, L., Haas, M., Merkle, T., Gilmer, D.: Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. The Journal of General Virology. 85, 2459-2469 (2004).
Vetter, Guillaume, Hily, Jean-Michel, Klein, Elodie, Schmidlin, Laure, Haas, Muriel, Merkle, Thomas, and Gilmer, David. “Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein”. The Journal of General Virology 85.8 (2004): 2459-2469.
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