Promoter inactivation or inhibition by sequence-specific methylation and mechanisms of reactivation

Doerfler W, Hoeveler A, Weisshaar B, Dobrzanski P, Knebel D, Langner KD, Achten S, Muller U (1989)
Cell Biophysics 15(1-2): 21-27.

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Abstract
In studies on adenovirus promoters, predominantly on the late E2A promoter of adenovirus type 2 (Ad2), we have demonstrated by a number of experimental approaches that the sequence-specific methylation of three 5'-CCGG-3' sequences inactivates this promoter. Recently, we have developed a cell-free transcription system in which the methylation-inactivation of eukaryotic promoters can be studied in detail. It has also been shown that methylation-caused promoter inactivation can be reversed by the 289 amino acid E1A protein of Ad2 or of adenovirus type 5. In the presence of this protein with a transactivating effect, transcription is initiated at the authentic cap site of the methylated late E2A promoter. A similar reactivation of the methylated late E2A promoter can also be effected by a cis-acting genetic element, i.e., the strong enhancer of human cytomegalovirus. Further studies will be directed toward the biochemical mechanisms of promoter silencing by sequence-specific methylations.
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Doerfler W, Hoeveler A, Weisshaar B, et al. Promoter inactivation or inhibition by sequence-specific methylation and mechanisms of reactivation. Cell Biophysics. 1989;15(1-2):21-27.
Doerfler, W., Hoeveler, A., Weisshaar, B., Dobrzanski, P., Knebel, D., Langner, K. D., Achten, S., et al. (1989). Promoter inactivation or inhibition by sequence-specific methylation and mechanisms of reactivation. Cell Biophysics, 15(1-2), 21-27.
Doerfler, W., Hoeveler, A., Weisshaar, B., Dobrzanski, P., Knebel, D., Langner, K. D., Achten, S., and Muller, U. (1989). Promoter inactivation or inhibition by sequence-specific methylation and mechanisms of reactivation. Cell Biophysics 15, 21-27.
Doerfler, W., et al., 1989. Promoter inactivation or inhibition by sequence-specific methylation and mechanisms of reactivation. Cell Biophysics, 15(1-2), p 21-27.
W. Doerfler, et al., “Promoter inactivation or inhibition by sequence-specific methylation and mechanisms of reactivation”, Cell Biophysics, vol. 15, 1989, pp. 21-27.
Doerfler, W., Hoeveler, A., Weisshaar, B., Dobrzanski, P., Knebel, D., Langner, K.D., Achten, S., Muller, U.: Promoter inactivation or inhibition by sequence-specific methylation and mechanisms of reactivation. Cell Biophysics. 15, 21-27 (1989).
Doerfler, W, Hoeveler, A, Weisshaar, Bernd, Dobrzanski, P, Knebel, D, Langner, K D, Achten, S, and Muller, U. “Promoter inactivation or inhibition by sequence-specific methylation and mechanisms of reactivation”. Cell Biophysics 15.1-2 (1989): 21-27.
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