Bacteriophage 2851 is a prototype phage for dissemination of the Shiga toxin variant gene 2c in Escherichia coli O157:H7

Strauch E, Hammerl JA, Konietzny A, Schneiker-Bekel S, Arnold W, Goesmann A, Pühler A, Beutin L (2008)
Infection and Immunity 76(12): 5466-5477.

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Abstract
The production of Shiga toxin (Stx) (verocytotoxin) is a major virulence factor of Escherichia coli O157:H7 strains (Shiga toxin-producing E. coli [STEC] O157). Two types of Shiga toxins, designated Stx1 and Stx2, are produced in STEC O157. Variants of the Stx2 type (Stx2, Stx2c) are associated with high virulences of these strains for humans. A bacteriophage designated 2851 from a human STEC O157 encoding the Stx2c variant was described previously. Nucleotide sequence analysis of the phage 2851 genome revealed 75 predicted coding sequences and indicated a mosaic structure typical for lambdoid phages. Analyses of free phages and K-12 phage 2851 lysogens revealed that upon excision from the bacterial chromosome, the loss of a phage-encoded IS629 element leads to fusion of phage antA and antB genes, with the generation of a recombined antAB gene encoding a strong antirepressor. In wild-type E. coli O157 as well as in K-12 strains, phage 2851 was found to be integrated in the sbcB locus. Additionally, phage 2851 carries an open reading frame which encodes an OspB-like type III effector similar to that found in Shigella spp. Investigation of 39 stx(2c) E. coli O157 strains revealed that all except 1 were positive for most phage 2851-specific genes and possessed a prophage with the same border sequences integrated into the sbcB locus. Phage 2851-specific sequences were absent from most stx(2c)-negative E. coli O157 strains, and we suggest that phage 2851-like phages contributed significantly to the dissemination of the Stx2c variant toxin within this group of E. coli.
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Strauch E, Hammerl JA, Konietzny A, et al. Bacteriophage 2851 is a prototype phage for dissemination of the Shiga toxin variant gene 2c in Escherichia coli O157:H7. Infection and Immunity. 2008;76(12):5466-5477.
Strauch, E., Hammerl, J. A., Konietzny, A., Schneiker-Bekel, S., Arnold, W., Goesmann, A., Pühler, A., et al. (2008). Bacteriophage 2851 is a prototype phage for dissemination of the Shiga toxin variant gene 2c in Escherichia coli O157:H7. Infection and Immunity, 76(12), 5466-5477.
Strauch, E., Hammerl, J. A., Konietzny, A., Schneiker-Bekel, S., Arnold, W., Goesmann, A., Pühler, A., and Beutin, L. (2008). Bacteriophage 2851 is a prototype phage for dissemination of the Shiga toxin variant gene 2c in Escherichia coli O157:H7. Infection and Immunity 76, 5466-5477.
Strauch, E., et al., 2008. Bacteriophage 2851 is a prototype phage for dissemination of the Shiga toxin variant gene 2c in Escherichia coli O157:H7. Infection and Immunity, 76(12), p 5466-5477.
E. Strauch, et al., “Bacteriophage 2851 is a prototype phage for dissemination of the Shiga toxin variant gene 2c in Escherichia coli O157:H7”, Infection and Immunity, vol. 76, 2008, pp. 5466-5477.
Strauch, E., Hammerl, J.A., Konietzny, A., Schneiker-Bekel, S., Arnold, W., Goesmann, A., Pühler, A., Beutin, L.: Bacteriophage 2851 is a prototype phage for dissemination of the Shiga toxin variant gene 2c in Escherichia coli O157:H7. Infection and Immunity. 76, 5466-5477 (2008).
Strauch, Eckhard, Hammerl, Jens Andre, Konietzny, Antje, Schneiker-Bekel, Susanne, Arnold, Walter, Goesmann, Alexander, Pühler, Alfred, and Beutin, Lothar. “Bacteriophage 2851 is a prototype phage for dissemination of the Shiga toxin variant gene 2c in Escherichia coli O157:H7”. Infection and Immunity 76.12 (2008): 5466-5477.
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55 References

Data provided by Europe PubMed Central.

Verocytotoxin-producing Escherichia coli (VTEC) O157 and other VTEC from human infections in England and Wales: 1995-1998.
Willshaw GA, Cheasty T, Smith HR, O'Brien SJ, Adak GK., J. Med. Microbiol. 50(2), 2001
PMID: 11211220
Vero cytotoxin-producing Escherichia coli O157 outbreaks in England and Wales, 1995: phenotypic methods and genotypic subtyping.
Willshaw GA, Smith HR, Cheasty T, Wall PG, Rowe B., Emerging Infect. Dis. 3(4), 1997
PMID: 9366610
Transcriptional analysis of genes encoding Shiga toxin 2 and its variants in Escherichia coli.
Zhang W, Bielaszewska M, Friedrich AW, Kuczius T, Karch H., Appl. Environ. Microbiol. 71(1), 2005
PMID: 15640236
Genotypic characterization and prevalence of virulence factors among Canadian Escherichia coli O157:H7 strains.
Ziebell K, Steele M, Zhang Y, Benson A, Taboada EN, Laing C, McEwen S, Ciebin B, Johnson R, Gannon V., Appl. Environ. Microbiol. 74(14), 2008
PMID: 18487402
OspF and OspC1 are Shigella flexneri type III secretion system effectors that are required for postinvasion aspects of virulence.
Zurawski DV, Mitsuhata C, Mumy KL, McCormick BA, Maurelli AT., Infect. Immun. 74(10), 2006
PMID: 16988276

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