DNA integrity, growth pattern, spindle formation, chromosomal constitution and imprinting patterns of mouse oocytes from vitrified pre-antral follicles

Trapphoff T, El Hajj N, Zechner U, Haaf T, Eichenlaub-Ritter U (2010)
Human Reproduction 25(12): 3025-3042.

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Cryopreservation of follicles for culture and oocyte growth and maturation in vitro provides an option to increase the number of fertilizable oocytes and restore fertility in cases where transplantation of ovarian tissue poses a risk for malignant cell contamination. Vitrification for cryopreservation is fast and avoids ice crystal formation. However, the influences of exposure to high concentrations of cryoprotectants on follicle development, oocyte growth and maturation, and particularly, on the DNA integrity and methylation imprinting has not been studied systematically. Follicle survival and development, DNA damage, oocyte growth patterns, maturation, spindle formation and chromosomal constitution were studied after Cryo-Top vitrification of mouse pre-antral follicles cultured to the antral stage and induced to ovulate in vitro. Methylation of differentially methylated regions (DMRs) of two maternally (Snrpn and Igf2r) and one paternally (H19) imprinted genes was studied by bisulfite pyrosequencing. Vitrification results in partial or total loss of oocyte-granulosa cell apposition and actin-rich transzonal projections, a transient increase in DNA breaks and a delay in follicle development. However, the oocyte growth pattern, maturation, spindle and chromosomal constitution are not significantly different between the vitrified and the control groups. Vitrification is not associated with elevated levels of imprinting mutations (aberrant methylation of the entire DMR), although the distribution of sporadic CpG methylation errors in the Snrpn DMR appears to differ slightly between control and vitrified oocytes. DNA breaks appear to be rapidly repaired and vitrification of oocytes inside pre-antral follicles by the Cryo-Top method does not appear to increase risks of abnormal imprinting or disturbances in spindle formation and chromosome segregation.
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Trapphoff T, El Hajj N, Zechner U, Haaf T, Eichenlaub-Ritter U. DNA integrity, growth pattern, spindle formation, chromosomal constitution and imprinting patterns of mouse oocytes from vitrified pre-antral follicles. Human Reproduction. 2010;25(12):3025-3042.
Trapphoff, T., El Hajj, N., Zechner, U., Haaf, T., & Eichenlaub-Ritter, U. (2010). DNA integrity, growth pattern, spindle formation, chromosomal constitution and imprinting patterns of mouse oocytes from vitrified pre-antral follicles. Human Reproduction, 25(12), 3025-3042. doi:10.1093/humrep/deq278
Trapphoff, T., El Hajj, N., Zechner, U., Haaf, T., and Eichenlaub-Ritter, U. (2010). DNA integrity, growth pattern, spindle formation, chromosomal constitution and imprinting patterns of mouse oocytes from vitrified pre-antral follicles. Human Reproduction 25, 3025-3042.
Trapphoff, T., et al., 2010. DNA integrity, growth pattern, spindle formation, chromosomal constitution and imprinting patterns of mouse oocytes from vitrified pre-antral follicles. Human Reproduction, 25(12), p 3025-3042.
T. Trapphoff, et al., “DNA integrity, growth pattern, spindle formation, chromosomal constitution and imprinting patterns of mouse oocytes from vitrified pre-antral follicles”, Human Reproduction, vol. 25, 2010, pp. 3025-3042.
Trapphoff, T., El Hajj, N., Zechner, U., Haaf, T., Eichenlaub-Ritter, U.: DNA integrity, growth pattern, spindle formation, chromosomal constitution and imprinting patterns of mouse oocytes from vitrified pre-antral follicles. Human Reproduction. 25, 3025-3042 (2010).
Trapphoff, Tom, El Hajj, Nady, Zechner, Ulrich, Haaf, Thomas, and Eichenlaub-Ritter, Ursula. “DNA integrity, growth pattern, spindle formation, chromosomal constitution and imprinting patterns of mouse oocytes from vitrified pre-antral follicles”. Human Reproduction 25.12 (2010): 3025-3042.
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PMID: 22965003
Culture of oocytes and risk of imprinting defects.
Anckaert E, De Rycke M, Smitz J., Hum Reprod Update 19(1), 2013
PMID: 23054129
Optimising vitrification of human oocytes using multiple cryoprotectants and morphological and functional assessment.
Seet VY, Al-Samerria S, Wong J, Stanger J, Yovich JL, Almahbobi G., Reprod Fertil Dev 25(6), 2013
PMID: 22967503
Effect of vitrification of mouse oocyte on the behavior of adult offspring.
Liu XJ, Jia GH, Zhang G, Tian KY, Wang HX, Zhong WY, Zhang YM, Lu XL, Li JT, Zhang JM., Eur J Obstet Gynecol Reprod Biol 169(2), 2013
PMID: 23743059
Oocyte cryopreservation: searching for novel improvement strategies.
Clark NA, Swain JE., J Assist Reprod Genet 30(7), 2013
PMID: 23779099
Compromised fertility disrupts Peg1 but not Snrpn and Peg3 imprinted methylation acquisition in mouse oocytes.
Denomme MM, White CR, Gillio-Meina C, Macdonald WA, Deroo BJ, Kidder GM, Mann MR., Front Genet 3(), 2012
PMID: 22798963
Epigenetic profile of developmentally important genes in bovine oocytes.
Heinzmann J, Hansmann T, Herrmann D, Wrenzycki C, Zechner U, Haaf T, Niemann H., Mol Reprod Dev 78(3), 2011
PMID: 21290475
Limiting dilution bisulfite (pyro)sequencing reveals parent-specific methylation patterns in single early mouse embryos and bovine oocytes.
El Hajj N, Trapphoff T, Linke M, May A, Hansmann T, Kuhtz J, Reifenberg K, Heinzmann J, Niemann H, Daser A, Eichenlaub-Ritter U, Zechner U, Haaf T., Epigenetics 6(10), 2011
PMID: 21937882

112 References

Data provided by Europe PubMed Central.


AUTHOR UNKNOWN, Cytogenetics 5(), 1966
Cryopreservation and oxidative stress in reproductive cells.
Tatone C, Di Emidio G, Vento M, Ciriminna R, Artini PG., Gynecol. Endocrinol. 26(8), 2010
PMID: 20230330
Cumulative ongoing pregnancy rate achieved with oocyte vitrification and cleavage stage transfer without embryo selection in a standard infertility program.
Ubaldi F, Anniballo R, Romano S, Baroni E, Albricci L, Colamaria S, Capalbo A, Sapienza F, Vajta G, Rienzi L., Hum. Reprod. 25(5), 2010
PMID: 20185513
Crosstalk between histone modifications during the DNA damage response.
van Attikum H, Gasser SM., Trends Cell Biol. 19(5), 2009
PMID: 19342239
Osmotic responses and tolerance limits to changes in external osmolalities, and oolemma permeability characteristics, of human in vitro matured MII oocytes.
Van den Abbeel E, Schneider U, Liu J, Agca Y, Critser JK, Van Steirteghem A., Hum. Reprod. 22(7), 2007
PMID: 17428880
Ovarian follicle bioassay reveals adverse effects of diazepam exposure upon follicle development and oocyte quality.
Van Wemmel K, Gobbers E, Eichenlaub-Ritter U, Smitz J, Cortvrindt R., Reprod. Toxicol. 20(2), 2005
PMID: 15907652
Spindle formation, chromosome segregation and the spindle checkpoint in mammalian oocytes and susceptibility to meiotic error.
Vogt E, Kirsch-Volders M, Parry J, Eichenlaub-Ritter U., Mutat. Res. 651(1-2), 2008
PMID: 18096427
Aurora kinase B, epigenetic state of centromeric heterochromatin and chiasma resolution in oocytes.
Vogt E, Kipp A, Eichenlaub-Ritter U., Reprod. Biomed. Online 19(3), 2009
PMID: 19778480
Preservation of fertility in children treated for cancer.
Wallace WH, Thomson AB., Arch. Dis. Child. 88(6), 2003
PMID: 12765914
Quantitative methylation analysis of developmentally important genes in human pregnancy losses after ART and spontaneous conception.
Zechner U, Pliushch G, Schneider E, El Hajj N, Tresch A, Shufaro Y, Seidmann L, Coerdt W, Muller AM, Haaf T., Mol. Hum. Reprod. 16(9), 2010
PMID: 20007506
Chromatin organization during mouse oocyte growth.
Zuccotti M, Piccinelli A, Giorgi Rossi P, Garagna S, Redi CA., Mol. Reprod. Dev. 41(4), 1995
PMID: 7576615

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