A family of serine proteases of Clavibacter michiganensis subsp. michiganensis: chpC plays a role in colonization of the host plant tomato

Stork I, Gartemann K-H, Burger A, Eichenlaub R (2008)
Molecular Plant Pathology 9(5): 599-608.

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Abstract
Genes for seven putative serine proteases (ChpA-ChpG) belonging to the trypsin subfamily and homologous to the virulence factor pat-1 were identified on the chromosome of Clavibacter michiganensis subsp. michiganensis (Cmm) NCPPB382. All proteases have signal peptides indicating export of these proteins. Their putative function is suggested by two motifs and an aspartate residue typical for serine proteases. Furthermore, six cysteine residues are located at conserved positions. The genes are clustered in a chromosomal region of about 50 kb with a significantly lower G + C content than common for Cmm. The genes chpA, chpB and chpD are pseudogenes as they contain frame shifts and/or in-frame stop codons. The genes chpC and chpG were inactivated by the insertion of an antibiotic resistance cassette. The chpG mutant was not impaired in virulence. However, in planta the titre of the chpC mutant was drastically reduced and only weak disease symptoms were observed. Complementation of the chpC mutant by the wild-type allele restored full virulence. ChpC is the first chromosomal gene of Cmm identified so far that affects the interaction of the pathogen with the host plant.
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Stork I, Gartemann K-H, Burger A, Eichenlaub R. A family of serine proteases of Clavibacter michiganensis subsp. michiganensis: chpC plays a role in colonization of the host plant tomato. Molecular Plant Pathology. 2008;9(5):599-608.
Stork, I., Gartemann, K. - H., Burger, A., & Eichenlaub, R. (2008). A family of serine proteases of Clavibacter michiganensis subsp. michiganensis: chpC plays a role in colonization of the host plant tomato. Molecular Plant Pathology, 9(5), 599-608.
Stork, I., Gartemann, K. - H., Burger, A., and Eichenlaub, R. (2008). A family of serine proteases of Clavibacter michiganensis subsp. michiganensis: chpC plays a role in colonization of the host plant tomato. Molecular Plant Pathology 9, 599-608.
Stork, I., et al., 2008. A family of serine proteases of Clavibacter michiganensis subsp. michiganensis: chpC plays a role in colonization of the host plant tomato. Molecular Plant Pathology, 9(5), p 599-608.
I. Stork, et al., “A family of serine proteases of Clavibacter michiganensis subsp. michiganensis: chpC plays a role in colonization of the host plant tomato”, Molecular Plant Pathology, vol. 9, 2008, pp. 599-608.
Stork, I., Gartemann, K.-H., Burger, A., Eichenlaub, R.: A family of serine proteases of Clavibacter michiganensis subsp. michiganensis: chpC plays a role in colonization of the host plant tomato. Molecular Plant Pathology. 9, 599-608 (2008).
Stork, Ines, Gartemann, Karl-Heinz, Burger, Annette, and Eichenlaub, Rudolf. “A family of serine proteases of Clavibacter michiganensis subsp. michiganensis: chpC plays a role in colonization of the host plant tomato”. Molecular Plant Pathology 9.5 (2008): 599-608.
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Tomato fruit and seed colonization by Clavibacter michiganensis subsp. michiganensis through external and internal routes.
Tancos MA, Chalupowicz L, Barash I, Manulis-Sasson S, Smart CD., Appl. Environ. Microbiol. 79(22), 2013
PMID: 24014525
Characterization of phenotypic variants of Clavibacter michiganensis subsp. michiganensis isolated from Capsicum annuum
Yim KO, Lee HI, Kim JH, Lee SD, Cho JH, Cha JS., Eur. J. Plant Pathol. 133(3), 2012
PMID: IND44685588
Colonization and movement of GFP-labeled Clavibacter michiganensis subsp. michiganensis during tomato infection.
Chalupowicz L, Zellermann EM, Fluegel M, Dror O, Eichenlaub R, Gartemann KH, Savidor A, Sessa G, Iraki N, Barash I, Manulis-Sasson S., Phytopathology 102(1), 2012
PMID: 21879791

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