Impact of profiling technologies in the understanding of recombinant protein production

Vijayendran C, Flaschel E (2010)
Adv Biochem Eng Biotechnol 121.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
;
Abstract / Bemerkung
Since expression profiling methods have been available in a high throughput fashion, the implication of these technologies in the field of biotechnology has increased dramatically. Microarray technology is one such unique and efficient methodology for simultaneous exploration of expression levels of numerous genes. Likewise, two-dimensional gel electrophoresis or multidimensional liquid chromatography coupled with mass spectrometry are extensively utilised for studying expression levels of numerous proteins. In the field of biotechnology these highly parallel analytical methods have paved the way to study and understand various biological phenomena depending on expression patterns. The next phenomenological level is represented by the metabolome and the (metabolic) fluxome. However, this chapter reviews gene and protein profiling and their impact on understanding recombinant protein production. We focus on the computational methods utilised for the analyses of data obtained from these profiling technologies as well as prominent results focusing on recombinant protein expression with Escherichia coli. Owing to the knowledge accumulated with respect to cellular signals triggered during recombinant protein production, this field is on the way to design strategies for developing improved processes. Both gene and protein profiling have exhibited a handful of functional categories to concentrate on in order to identify target genes and proteins, respectively, involved in the signalling network with major impact on recombinant protein production.
Erscheinungsjahr
Zeitschriftentitel
Adv Biochem Eng Biotechnol
Band
121
PUB-ID

Zitieren

Vijayendran C, Flaschel E. Impact of profiling technologies in the understanding of recombinant protein production. Adv Biochem Eng Biotechnol. 2010;121.
Vijayendran, C., & Flaschel, E. (2010). Impact of profiling technologies in the understanding of recombinant protein production. Adv Biochem Eng Biotechnol, 121. doi:10.1007/10_2009_56
Vijayendran, C., and Flaschel, E. (2010). Impact of profiling technologies in the understanding of recombinant protein production. Adv Biochem Eng Biotechnol 121.
Vijayendran, C., & Flaschel, E., 2010. Impact of profiling technologies in the understanding of recombinant protein production. Adv Biochem Eng Biotechnol, 121.
C. Vijayendran and E. Flaschel, “Impact of profiling technologies in the understanding of recombinant protein production”, Adv Biochem Eng Biotechnol, vol. 121, 2010.
Vijayendran, C., Flaschel, E.: Impact of profiling technologies in the understanding of recombinant protein production. Adv Biochem Eng Biotechnol. 121, (2010).
Vijayendran, Chandran, and Flaschel, Erwin. “Impact of profiling technologies in the understanding of recombinant protein production”. Adv Biochem Eng Biotechnol 121 (2010).

1 Zitation in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 20186528
PubMed | Europe PMC

Suchen in

Google Scholar