High resolution imaging of surface patterns of single bacterial cells

Greif D, Wesner D, Regtmeier J, Anselmetti D (2010)
ULTRAMICROSCOPY 110(10): 1290-1296.

Journal Article | Published | English

No fulltext has been uploaded

Abstract
We systematically studied the origin of surface patterns observed on single Sinorhizobium meliloti bacterial cells by comparing the complementary techniques atomic force microscopy (AFM) and scanning electron microscopy (SEM). Conditions ranged from living bacteria in liquid to fixed bacteria in high vacuum. Stepwise, we applied different sample modifications (fixation, drying, metal coating, etc.) and characterized the observed surface patterns. A detailed analysis revealed that the surface structure with wrinkled protrusions in SEM images were not generated de novo but most likely evolved from similar and naturally present structures on the surface of living bacteria. The influence of osmotic stress to the surface structure of living cells was evaluated and also the contribution of exopolysaccharide and lipopolysaccharide (LPS) by imaging two mutant strains of the bacterium under native conditions. AFM images of living bacteria in culture medium exhibited surface structures of the size of single proteins emphasizing the usefulness of AFM for high resolution cell imaging. (C) 2010 Elsevier B.V. All rights reserved.
Publishing Year
ISSN
PUB-ID

Cite this

Greif D, Wesner D, Regtmeier J, Anselmetti D. High resolution imaging of surface patterns of single bacterial cells. ULTRAMICROSCOPY. 2010;110(10):1290-1296.
Greif, D., Wesner, D., Regtmeier, J., & Anselmetti, D. (2010). High resolution imaging of surface patterns of single bacterial cells. ULTRAMICROSCOPY, 110(10), 1290-1296.
Greif, D., Wesner, D., Regtmeier, J., and Anselmetti, D. (2010). High resolution imaging of surface patterns of single bacterial cells. ULTRAMICROSCOPY 110, 1290-1296.
Greif, D., et al., 2010. High resolution imaging of surface patterns of single bacterial cells. ULTRAMICROSCOPY, 110(10), p 1290-1296.
D. Greif, et al., “High resolution imaging of surface patterns of single bacterial cells”, ULTRAMICROSCOPY, vol. 110, 2010, pp. 1290-1296.
Greif, D., Wesner, D., Regtmeier, J., Anselmetti, D.: High resolution imaging of surface patterns of single bacterial cells. ULTRAMICROSCOPY. 110, 1290-1296 (2010).
Greif, Dominik, Wesner, Daniel, Regtmeier, Jan, and Anselmetti, Dario. “High resolution imaging of surface patterns of single bacterial cells”. ULTRAMICROSCOPY 110.10 (2010): 1290-1296.
This data publication is cited in the following publications:
This publication cites the following data publications:

9 Citations in Europe PMC

Data provided by Europe PubMed Central.

Conjugated gold nanoparticles as a tool for probing the bacterial cell envelope: The case of Shewanella oneidensis MR-1.
Jahnke JP, Cornejo JA, Sumner JJ, Schuler AJ, Atanassov P, Ista LK., Biointerphases 11(1), 2016
PMID: 26746161
Antibacterial Activity of Cinnamaldehyde and Sporan against Escherichia coli�� O157:H7 and Salmonella
Yossa N, Patel J, Macarisin D, Millner P, Murphy C, Bauchan G, Lo YM., 2014
PMID: IND500903718
The basement membrane of the isolated rat colonic mucosa. A light, electron and atomic force microscopy study.
Mestres P, Gomez LL, Lopez TN, del Rosario G, Lukas SW, Hartmann U., Ann. Anat. 196(2-3), 2014
PMID: 24582060
Monitoring bacterial growth using tunable resistive pulse sensing with a pore-based technique.
Yu AC, Loo JF, Yu S, Kong SK, Chan TF., Appl. Microbiol. Biotechnol. 98(2), 2014
PMID: 24287933
Atomic force microscopy of bacteria from periodontal subgingival biofilm: Preliminary study results.
Germano F, Bramanti E, Arcuri C, Cecchetti F, Cicciu M., Eur J Dent 7(2), 2013
PMID: 24883019
Atomic force microscopy with sub-picoNewton force stability for biological applications.
Sullan RM, Churnside AB, Nguyen DM, Bull MS, Perkins TT., Methods 60(2), 2013
PMID: 23562681
Automated force volume image processing for biological samples.
Polyakov P, Soussen C, Duan J, Duval JF, Brie D, Francius G., PLoS ONE 6(4), 2011
PMID: 21559483
Chemical analysis of single cells.
Lin Y, Trouillon R, Safina G, Ewing AG., Anal. Chem. 83(12), 2011
PMID: 21500835

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 20558001
PubMed | Europe PMC

Search this title in

Google Scholar