High resolution imaging of surface patterns of single bacterial cells

Greif D, Wesner D, Regtmeier J, Anselmetti D (2010)
ULTRAMICROSCOPY 110(10): 1290-1296.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
We systematically studied the origin of surface patterns observed on single Sinorhizobium meliloti bacterial cells by comparing the complementary techniques atomic force microscopy (AFM) and scanning electron microscopy (SEM). Conditions ranged from living bacteria in liquid to fixed bacteria in high vacuum. Stepwise, we applied different sample modifications (fixation, drying, metal coating, etc.) and characterized the observed surface patterns. A detailed analysis revealed that the surface structure with wrinkled protrusions in SEM images were not generated de novo but most likely evolved from similar and naturally present structures on the surface of living bacteria. The influence of osmotic stress to the surface structure of living cells was evaluated and also the contribution of exopolysaccharide and lipopolysaccharide (LPS) by imaging two mutant strains of the bacterium under native conditions. AFM images of living bacteria in culture medium exhibited surface structures of the size of single proteins emphasizing the usefulness of AFM for high resolution cell imaging. (C) 2010 Elsevier B.V. All rights reserved.
Erscheinungsjahr
Zeitschriftentitel
ULTRAMICROSCOPY
Band
110
Zeitschriftennummer
10
Seite
1290-1296
ISSN
PUB-ID

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Greif D, Wesner D, Regtmeier J, Anselmetti D. High resolution imaging of surface patterns of single bacterial cells. ULTRAMICROSCOPY. 2010;110(10):1290-1296.
Greif, D., Wesner, D., Regtmeier, J., & Anselmetti, D. (2010). High resolution imaging of surface patterns of single bacterial cells. ULTRAMICROSCOPY, 110(10), 1290-1296. doi:10.1016/j.ultramic.2010.06.004
Greif, D., Wesner, D., Regtmeier, J., and Anselmetti, D. (2010). High resolution imaging of surface patterns of single bacterial cells. ULTRAMICROSCOPY 110, 1290-1296.
Greif, D., et al., 2010. High resolution imaging of surface patterns of single bacterial cells. ULTRAMICROSCOPY, 110(10), p 1290-1296.
D. Greif, et al., “High resolution imaging of surface patterns of single bacterial cells”, ULTRAMICROSCOPY, vol. 110, 2010, pp. 1290-1296.
Greif, D., Wesner, D., Regtmeier, J., Anselmetti, D.: High resolution imaging of surface patterns of single bacterial cells. ULTRAMICROSCOPY. 110, 1290-1296 (2010).
Greif, Dominik, Wesner, Daniel, Regtmeier, Jan, and Anselmetti, Dario. “High resolution imaging of surface patterns of single bacterial cells”. ULTRAMICROSCOPY 110.10 (2010): 1290-1296.

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Franz J, Grünebaum J, Schäfer M, Mulac D, Rehfeldt F, Langer K, Kramer A, Riethmüller C., PLoS One 13(1), 2018
PMID: 29320535
Phenotypic and Physiological Characterization of the Epibiotic Interaction Between TM7x and Its Basibiont Actinomyces.
Bor B, Poweleit N, Bois JS, Cen L, Bedree JK, Zhou ZH, Gunsalus RP, Lux R, McLean JS, He X, Shi W., Microb Ecol 71(1), 2016
PMID: 26597961
Characterization of antibiotic resistant and enzyme producing bacterial strains isolated from the Arabian Sea.
Tallur PN, Sajjan DB, Mulla SI, Talwar MP, Pragasam A, Nayak VM, Ninnekar HZ, Bhat SS., 3 Biotech 6(1), 2016
PMID: 28330094
Conjugated gold nanoparticles as a tool for probing the bacterial cell envelope: The case of Shewanella oneidensis MR-1.
Jahnke JP, Cornejo JA, Sumner JJ, Schuler AJ, Atanassov P, Ista LK., Biointerphases 11(1), 2016
PMID: 26746161
Monitoring bacterial growth using tunable resistive pulse sensing with a pore-based technique.
Yu AC, Loo JF, Yu S, Kong SK, Chan TF., Appl Microbiol Biotechnol 98(2), 2014
PMID: 24287933
The basement membrane of the isolated rat colonic mucosa. A light, electron and atomic force microscopy study.
Mestres P, Gomez LL, Lopez TN, del Rosario G, Lukas SW, Hartmann U., Ann Anat 196(2-3), 2014
PMID: 24582060
Antibacterial Activity of Cinnamaldehyde and Sporan against Escherichia coli�� O157:H7 and Salmonella
Yossa N, Patel J, Macarisin D, Millner P, Murphy C, Bauchan G, Lo YM., Journal of food processing and preservation. 38(3), 2014
PMID: IND500903718
Atomic force microscopy of bacteria from periodontal subgingival biofilm: Preliminary study results.
Germano F, Bramanti E, Arcuri C, Cecchetti F, Cicciù M., Eur J Dent 7(2), 2013
PMID: 24883019
Atomic force microscopy with sub-picoNewton force stability for biological applications.
Sullan RM, Churnside AB, Nguyen DM, Bull MS, Perkins TT., Methods 60(2), 2013
PMID: 23562681
Chemical analysis of single cells.
Lin Y, Trouillon R, Safina G, Ewing AG., Anal Chem 83(12), 2011
PMID: 21500835
Automated force volume image processing for biological samples.
Polyakov P, Soussen C, Duan J, Duval JF, Brie D, Francius G., PLoS One 6(4), 2011
PMID: 21559483

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