Global transcript profiling of transgenic plants constitutively overexpressing the RNA-binding protein AtGRP7

Streitner C, Hennig L, Korneli C, Staiger D (2010)
BMC Plant Biology 10(1).

Download
OA
Journal Article | Published | English
Abstract
Background: The clock-controlled RNA-binding protein AtGRP7 influences circadian oscillations of its own transcript at the post-transcriptional level. To identify additional targets that are regulated by AtGRP7, transcript profiles of transgenic plants constitutively overexpressing AtGRP7 (AtGRP7-ox) and wild type plants were compared. Results: Approximately 1.4% of the transcripts represented on the Affymetrix ATH1 microarray showed changes in steady-state abundance upon AtGRP7 overexpression. One third of the differentially expressed genes are controlled by the circadian clock, and they show a distinct bias of their phase: The up-regulated genes preferentially peak around dawn, roughly opposite to the AtGRP7 peak abundance whereas the down-regulated genes preferentially peak at the end of the day. Further, transcripts responsive to abiotic and biotic stimuli were enriched among AtGRP7 targets. Transcripts encoding the pathogenesis-related PR1 and PR2 proteins were elevated in AtGRP7-ox plants but not in plants overexpressing AtGRP7 with a point mutation in the RNA-binding domain, indicating that the regulation involves RNA binding activity of AtGRP7. Gene set enrichment analysis uncovered components involved in ribosome function and RNA metabolism among groups of genes upregulated in AtGRP7-ox plants, consistent with its role in post-transcriptional regulation. Conclusion: Apart from regulating a suite of circadian transcripts in a time-of-day dependent manner AtGRP7, both directly and indirectly, affects other transcripts including transcripts responsive to abiotic and biotic stimuli. This suggests a regulatory role of AtGRP7 in the output of the endogenous clock and a complex network of transcripts responsive to external stimuli downstream of the AtGRP7 autoregulatory circuit.
Publishing Year
ISSN
PUB-ID

Cite this

Streitner C, Hennig L, Korneli C, Staiger D. Global transcript profiling of transgenic plants constitutively overexpressing the RNA-binding protein AtGRP7. BMC Plant Biology. 2010;10(1).
Streitner, C., Hennig, L., Korneli, C., & Staiger, D. (2010). Global transcript profiling of transgenic plants constitutively overexpressing the RNA-binding protein AtGRP7. BMC Plant Biology, 10(1).
Streitner, C., Hennig, L., Korneli, C., and Staiger, D. (2010). Global transcript profiling of transgenic plants constitutively overexpressing the RNA-binding protein AtGRP7. BMC Plant Biology 10.
Streitner, C., et al., 2010. Global transcript profiling of transgenic plants constitutively overexpressing the RNA-binding protein AtGRP7. BMC Plant Biology, 10(1).
C. Streitner, et al., “Global transcript profiling of transgenic plants constitutively overexpressing the RNA-binding protein AtGRP7”, BMC Plant Biology, vol. 10, 2010.
Streitner, C., Hennig, L., Korneli, C., Staiger, D.: Global transcript profiling of transgenic plants constitutively overexpressing the RNA-binding protein AtGRP7. BMC Plant Biology. 10, (2010).
Streitner, Corinna, Hennig, Lars, Korneli, Christin, and Staiger, Dorothee. “Global transcript profiling of transgenic plants constitutively overexpressing the RNA-binding protein AtGRP7”. BMC Plant Biology 10.1 (2010).
Main File(s)
File Name
Access Level
OA Open Access

This data publication is cited in the following publications:
This publication cites the following data publications:

16 Citations in Europe PMC

Data provided by Europe PubMed Central.

RNA around the clock - regulation at the RNA level in biological timing.
Nolte C, Staiger D., Front Plant Sci 6(), 2015
PMID: 25999975
Environmental stresses modulate abundance and timing of alternatively spliced circadian transcripts in Arabidopsis.
Filichkin SA, Cumbie JS, Dharmawardhana P, Jaiswal P, Chang JH, Palusa SG, Reddy AS, Megraw M, Mockler TC., Mol Plant 8(2), 2015
PMID: 25680774
Time to flower: interplay between photoperiod and the circadian clock.
Johansson M, Staiger D., J. Exp. Bot. 66(3), 2015
PMID: 25371508
Regulation of pri-miRNA processing by the hnRNP-like protein AtGRP7 in Arabidopsis.
Koster T, Meyer K, Weinholdt C, Smith LM, Lummer M, Speth C, Grosse I, Weigel D, Staiger D., Nucleic Acids Res. 42(15), 2014
PMID: 25104024
A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis.
Lohr B, Streitner C, Steffen A, Lange T, Staiger D., Mol. Biol. Rep. 41(1), 2014
PMID: 24281950
Salicylic acid-dependent and -independent impact of an RNA-binding protein on plant immunity.
Hackmann C, Korneli C, Kutyniok M, Koster T, Wiedenlubbert M, Muller C, Staiger D., Plant Cell Environ. 37(3), 2014
PMID: 23961939
Plant RNA binding proteins for control of RNA virus infection.
Huh SU, Paek KH., Front Physiol 4(), 2013
PMID: 24427141
The circadian clock goes genomic.
Staiger D, Shin J, Johansson M, Davis SJ., Genome Biol. 14(6), 2013
PMID: 23796230
Ribonucleoprotein complexes that control circadian clocks.
Wang D, Liang X, Chen X, Guo J., Int J Mol Sci 14(5), 2013
PMID: 23698761
A new set of reversibly photoswitchable fluorescent proteins for use in transgenic plants.
Lummer M, Humpert F, Wiedenlubbert M, Sauer M, Schuttpelz M, Staiger D., Mol Plant 6(5), 2013
PMID: 23434876
Emerging role for RNA-based regulation in plant immunity.
Staiger D, Korneli C, Lummer M, Navarro L., New Phytol. 197(2), 2013
PMID: 23163405
An hnRNP-like RNA-binding protein affects alternative splicing by in vivo interaction with transcripts in Arabidopsis thaliana.
Streitner C, Koster T, Simpson CG, Shaw P, Danisman S, Brown JW, Staiger D., Nucleic Acids Res. 40(22), 2012
PMID: 23042250
Structure function analysis of an ADP-ribosyltransferase type III effector and its RNA-binding target in plant immunity.
Jeong BR, Lin Y, Joe A, Guo M, Korneli C, Yang H, Wang P, Yu M, Cerny RL, Staiger D, Alfano JR, Xu Y., J. Biol. Chem. 286(50), 2011
PMID: 22013065

63 References

Data provided by Europe PubMed Central.

Identification of target mRNAs for the clock-controlled RNA-binding protein Chlamy 1 from Chlamydomonas reinhardtii.
Waltenberger H, Schneid C, Grosch JO, Bareiss A, Mittag M., Mol. Genet. Genomics 265(1), 2001
PMID: 11370865
Mouse period 2 mRNA circadian oscillation is modulated by PTB-mediated rhythmic mRNA degradation.
Woo KC, Kim TD, Lee KH, Kim DY, Kim W, Lee KY, Kim KT., Nucleic Acids Res. 37(1), 2009
PMID: 19010962
A revised medium for rapid growth and bio assays with tobacco tissue cultures
AUTHOR UNKNOWN, 1962
A model based background adjustement for oligonucleotide expression arrays
AUTHOR UNKNOWN, 2004

AUTHOR UNKNOWN, 2009
The Polycomb-group protein MEDEA regulates seed development by controlling expression of the MADS-box gene PHERES1.
Kohler C, Hennig L, Spillane C, Pien S, Gruissem W, Grossniklaus U., Genes Dev. 17(12), 2003
PMID: 12815071
Controlling the False Discovery Rate: A Practical and Powerful Approach to Multiple Testing
AUTHOR UNKNOWN, 1995
Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis.
Czechowski T, Stitt M, Altmann T, Udvardi MK, Scheible WR., Plant Physiol. 139(1), 2005
PMID: 16166256

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 20946635
PubMed | Europe PMC

Search this title in

Google Scholar