Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints

Schmoll T, Janzon V, Epplen JT, Lubjuhn T (2003)
ELECTROPHORESIS 24(16): 2758-2763.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Molecular analyses of genetic parentage in wild bird populations demonstrate that extra-pair paternity (EPP) is common, even in socially monogamous species. After having identified extra-pair offspring (EPO), an important step is to uncover the identity of extra-pair sires (EPS). Studies relying on multilocus DNA fingerprinting face the problem that simple between-gel comparisons of fingerprint banding patterns are imprecise in assigning parentage across gels. To tackle this problem, we developed a method to identify EPS by means of standardized across-gel comparisons of multilocus DNA fingerprints and applied it in a socially monogamous bird species with high levels of EPP, the coal tit (Parus ater). Scanned DNA fingerprint gels and standard image-editing software allowed to screen and reliably identify EPS. Overall, EPP was successfully assigned to 32 different EPS for 143 out of 210 (68.1%) EPO detected. This corresponds well to extra-pair parentage assignment rates revealed by microsatellite-based studies in congeneric species. Our approach proves especially useful whenever multilocus DNA fingerprints for parentage exclusion exist while microsatellite markers are not available.
Erscheinungsjahr
Zeitschriftentitel
ELECTROPHORESIS
Band
24
Zeitschriftennummer
16
Seite
2758-2763
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Schmoll T, Janzon V, Epplen JT, Lubjuhn T. Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints. ELECTROPHORESIS. 2003;24(16):2758-2763.
Schmoll, T., Janzon, V., Epplen, J. T., & Lubjuhn, T. (2003). Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints. ELECTROPHORESIS, 24(16), 2758-2763. doi:10.1002/elps.200305571
Schmoll, T., Janzon, V., Epplen, J. T., and Lubjuhn, T. (2003). Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints. ELECTROPHORESIS 24, 2758-2763.
Schmoll, T., et al., 2003. Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints. ELECTROPHORESIS, 24(16), p 2758-2763.
T. Schmoll, et al., “Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints”, ELECTROPHORESIS, vol. 24, 2003, pp. 2758-2763.
Schmoll, T., Janzon, V., Epplen, J.T., Lubjuhn, T.: Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints. ELECTROPHORESIS. 24, 2758-2763 (2003).
Schmoll, Tim, Janzon, V, Epplen, JT, and Lubjuhn, T. “Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints”. ELECTROPHORESIS 24.16 (2003): 2758-2763.

2 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Comprehensive paternity assignment: genotype, spatial location and social status in song sparrows, Melospiza Melodia.
Sardell RJ, Keller LF, Arcese P, Bucher T, Reid JM., Mol Ecol 19(19), 2010
PMID: 20819155
Polyandry in coal tits Parus ater: fitness consequences of putting eggs into multiple genetic baskets.
Schmoll T, Schurr FM, Winkel W, Epplen JT, Lubjuhn T., J Evol Biol 20(3), 2007
PMID: 17465921

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