Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints

Schmoll T, Janzon V, Epplen JT, Lubjuhn T (2003)
ELECTROPHORESIS 24(16): 2758-2763.

Journal Article | Published | English

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Abstract
Molecular analyses of genetic parentage in wild bird populations demonstrate that extra-pair paternity (EPP) is common, even in socially monogamous species. After having identified extra-pair offspring (EPO), an important step is to uncover the identity of extra-pair sires (EPS). Studies relying on multilocus DNA fingerprinting face the problem that simple between-gel comparisons of fingerprint banding patterns are imprecise in assigning parentage across gels. To tackle this problem, we developed a method to identify EPS by means of standardized across-gel comparisons of multilocus DNA fingerprints and applied it in a socially monogamous bird species with high levels of EPP, the coal tit (Parus ater). Scanned DNA fingerprint gels and standard image-editing software allowed to screen and reliably identify EPS. Overall, EPP was successfully assigned to 32 different EPS for 143 out of 210 (68.1%) EPO detected. This corresponds well to extra-pair parentage assignment rates revealed by microsatellite-based studies in congeneric species. Our approach proves especially useful whenever multilocus DNA fingerprints for parentage exclusion exist while microsatellite markers are not available.
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Schmoll T, Janzon V, Epplen JT, Lubjuhn T. Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints. ELECTROPHORESIS. 2003;24(16):2758-2763.
Schmoll, T., Janzon, V., Epplen, J. T., & Lubjuhn, T. (2003). Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints. ELECTROPHORESIS, 24(16), 2758-2763.
Schmoll, T., Janzon, V., Epplen, J. T., and Lubjuhn, T. (2003). Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints. ELECTROPHORESIS 24, 2758-2763.
Schmoll, T., et al., 2003. Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints. ELECTROPHORESIS, 24(16), p 2758-2763.
T. Schmoll, et al., “Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints”, ELECTROPHORESIS, vol. 24, 2003, pp. 2758-2763.
Schmoll, T., Janzon, V., Epplen, J.T., Lubjuhn, T.: Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints. ELECTROPHORESIS. 24, 2758-2763 (2003).
Schmoll, Tim, Janzon, V, Epplen, JT, and Lubjuhn, T. “Extra-pair sires as identified by means of standardized across-gel comparisons of multilocus DNA fingerprints”. ELECTROPHORESIS 24.16 (2003): 2758-2763.
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2 Citations in Europe PMC

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Comprehensive paternity assignment: genotype, spatial location and social status in song sparrows, Melospiza Melodia.
Sardell RJ, Keller LF, Arcese P, Bucher T, Reid JM., Mol. Ecol. 19(19), 2010
PMID: 20819155
Polyandry in coal tits Parus ater: fitness consequences of putting eggs into multiple genetic baskets.
Schmoll T, Schurr FM, Winkel W, Epplen JT, Lubjuhn T., J. Evol. Biol. 20(3), 2007
PMID: 17465921

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