Reporter genes and highly regulated promoters as tools for transformation experiments in Volvox carteri

Hallmann A, Sumper M (1994)
Proc. Natl. Acad. Sci. USA 91: 11562-11566.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
;
Abstract / Bemerkung
The multicellular alga Volvox is an attractive model for the study of developmental processes. With the recent report of successful transformation, regulated promoters as well as reporter genes working in this organism are now required. The Volvox genes encoding arylsulfatase and the extracellular glycoprotein ISG are strictly regulated. The former is transcribed only under conditions of sulfur starvation, whereas the latter operates under extreme developmental control--i.e., it is transcribed for only a few minutes in Volvox embryos at the stage of embryonic inversion. The gene encoding the sexual pheromone of Volvox carteri was placed under the control of the arylsulfatase promoter. In response to sulfur deprivation, V. carteri transformed by this construct synthesized and secreted biologically active pheromone. In addition, the gene encoding Volvox arylsulfatase was placed under the control of the ISG promoter. Transformed algae synthesized arylsulfatase mRNA only during embryonic inversion. These experiments demonstrate the usefulness of both the arylsulfatase and the sexual pheromone reporter genes. In addition, the highly regulated arylsulfatase promoter allows the construction of inducible expression vectors for cloned genes.
Erscheinungsjahr
Zeitschriftentitel
Proc. Natl. Acad. Sci. USA
Band
91
Seite
11562-11566
PUB-ID

Zitieren

Hallmann A, Sumper M. Reporter genes and highly regulated promoters as tools for transformation experiments in Volvox carteri. Proc. Natl. Acad. Sci. USA. 1994;91:11562-11566.
Hallmann, A., & Sumper, M. (1994). Reporter genes and highly regulated promoters as tools for transformation experiments in Volvox carteri. Proc. Natl. Acad. Sci. USA, 91, 11562-11566.
Hallmann, A., and Sumper, M. (1994). Reporter genes and highly regulated promoters as tools for transformation experiments in Volvox carteri. Proc. Natl. Acad. Sci. USA 91, 11562-11566.
Hallmann, A., & Sumper, M., 1994. Reporter genes and highly regulated promoters as tools for transformation experiments in Volvox carteri. Proc. Natl. Acad. Sci. USA, 91, p 11562-11566.
A. Hallmann and M. Sumper, “Reporter genes and highly regulated promoters as tools for transformation experiments in Volvox carteri”, Proc. Natl. Acad. Sci. USA, vol. 91, 1994, pp. 11562-11566.
Hallmann, A., Sumper, M.: Reporter genes and highly regulated promoters as tools for transformation experiments in Volvox carteri. Proc. Natl. Acad. Sci. USA. 91, 11562-11566 (1994).
Hallmann, Armin, and Sumper, Manfred. “Reporter genes and highly regulated promoters as tools for transformation experiments in Volvox carteri”. Proc. Natl. Acad. Sci. USA 91 (1994): 11562-11566.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Suchen in

Google Scholar