Impaired lysosomal trimming of N-linked oligosaccharides leads to hyperglycosylation of native lysosomal proteins in mice with alpha-mannosidosis.

Damme M, Morelle W, Schmidt B, Andersson C, Fogh J, Michalski J-C, Lübke T (2010)
Molecular and Cellular Biology 30(1): 273-283.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ;
Abstract
Alpha-mannosidosis is caused by the genetic defect of the lysosomal alpha-d-mannosidase (LAMAN), which is involved in the breakdown of free alpha-linked mannose-containing oligosaccharides originating from glycoproteins with N-linked glycans, and thus manifests itself in an extensive storage of mannose-containing oligosaccharides. Here we demonstrate in a model of mice with alpha-mannosidosis that native lysosomal proteins exhibit elongated N-linked oligosaccharides as shown by two-dimensional difference gel electrophoresis, deglycosylation assays, and mass spectrometry. The analysis of cathepsin B-derived oligosaccharides revealed a hypermannosylation of glycoproteins in mice with alpha-mannosidosis as indicated by the predominance of extended Man3GlcNAc2 oligosaccharides. Treatment with recombinant human alpha-mannosidase partially corrected the hyperglycosylation of lysosomal proteins in vivo and in vitro. These data clearly demonstrate that LAMAN is involved not only in the lysosomal catabolism of free oligosaccharides but also in the trimming of asparagine-linked oligosaccharides on native lysosomal proteins.
Publishing Year
ISSN
PUB-ID

Cite this

Damme M, Morelle W, Schmidt B, et al. Impaired lysosomal trimming of N-linked oligosaccharides leads to hyperglycosylation of native lysosomal proteins in mice with alpha-mannosidosis. Molecular and Cellular Biology. 2010;30(1):273-283.
Damme, M., Morelle, W., Schmidt, B., Andersson, C., Fogh, J., Michalski, J. - C., & Lübke, T. (2010). Impaired lysosomal trimming of N-linked oligosaccharides leads to hyperglycosylation of native lysosomal proteins in mice with alpha-mannosidosis. Molecular and Cellular Biology, 30(1), 273-283.
Damme, M., Morelle, W., Schmidt, B., Andersson, C., Fogh, J., Michalski, J. - C., and Lübke, T. (2010). Impaired lysosomal trimming of N-linked oligosaccharides leads to hyperglycosylation of native lysosomal proteins in mice with alpha-mannosidosis. Molecular and Cellular Biology 30, 273-283.
Damme, M., et al., 2010. Impaired lysosomal trimming of N-linked oligosaccharides leads to hyperglycosylation of native lysosomal proteins in mice with alpha-mannosidosis. Molecular and Cellular Biology, 30(1), p 273-283.
M. Damme, et al., “Impaired lysosomal trimming of N-linked oligosaccharides leads to hyperglycosylation of native lysosomal proteins in mice with alpha-mannosidosis.”, Molecular and Cellular Biology, vol. 30, 2010, pp. 273-283.
Damme, M., Morelle, W., Schmidt, B., Andersson, C., Fogh, J., Michalski, J.-C., Lübke, T.: Impaired lysosomal trimming of N-linked oligosaccharides leads to hyperglycosylation of native lysosomal proteins in mice with alpha-mannosidosis. Molecular and Cellular Biology. 30, 273-283 (2010).
Damme, Markus, Morelle, Willy, Schmidt, Bernhard, Andersson, Claes, Fogh, Jens, Michalski, Jean-Claude, and Lübke, Torben. “Impaired lysosomal trimming of N-linked oligosaccharides leads to hyperglycosylation of native lysosomal proteins in mice with alpha-mannosidosis.”. Molecular and Cellular Biology 30.1 (2010): 273-283.
This data publication is cited in the following publications:
This publication cites the following data publications:

7 Citations in Europe PMC

Data provided by Europe PubMed Central.

Chronic enzyme replacement therapy ameliorates neuropathology in alpha-mannosidosis mice.
Damme M, Stroobants S, Ludemann M, Rothaug M, Lullmann-Rauch R, Beck HC, Ericsson A, Andersson C, Fogh J, D'Hooge R, Saftig P, Blanz J., Ann Clin Transl Neurol 2(11), 2015
PMID: 26817023
The accessory Sec protein Asp2 modulates GlcNAc deposition onto the serine-rich repeat glycoprotein GspB.
Seepersaud R, Bensing BA, Yen YT, Sullam PM., J. Bacteriol. 194(20), 2012
PMID: 22885294
Structural and mechanistic insight into N-glycan processing by endo-α-mannosidase.
Thompson AJ, Williams RJ, Hakki Z, Alonzi DS, Wennekes T, Gloster TM, Songsrirote K, Thomas-Oates JE, Wrodnigg TM, Spreitz J, Stutz AE, Butters TD, Williams SJ, Davies GJ., Proc. Natl. Acad. Sci. U.S.A. 109(3), 2012
PMID: 22219371
Mannose 6 dephosphorylation of lysosomal proteins mediated by acid phosphatases Acp2 and Acp5.
Makrypidi G, Damme M, Muller-Loennies S, Trusch M, Schmidt B, Schluter H, Heeren J, Lubke T, Saftig P, Braulke T., Mol. Cell. Biol. 32(4), 2012
PMID: 22158965
Cerebellar alterations and gait defects as therapeutic outcome measures for enzyme replacement therapy in α-mannosidosis.
Damme M, Stroobants S, Walkley SU, Lullmann-Rauch R, D'Hooge R, Fogh J, Saftig P, Lubke T, Blanz J., J. Neuropathol. Exp. Neurol. 70(1), 2011
PMID: 21157375

61 References

Data provided by Europe PubMed Central.

Structural studies on the carbohydrate moieties of rat liver cathepsins B and H.
Taniguchi T, Mizuochi T, Towatari T, Katunuma N, Kobata A., J. Biochem. 97(3), 1985
PMID: 4019443
Lysosomal cysteine proteases: facts and opportunities.
Turk V, Turk B, Turk D., EMBO J. 20(17), 2001
PMID: 11532926
Function of oligosaccharide modification in glucocerebrosidase, a membrane-associated lysosomal hydrolase.
Van Weely S, Aerts JM, Van Leeuwen MB, Heikoop JC, Donker-Koopman WE, Barranger JA, Tager JM, Schram AW., Eur. J. Biochem. 191(3), 1990
PMID: 2143986
Alterations in neuron morphology in feline mannosidosis. A Golgi study.
Walkley SU, Blakemore WF, Purpura DP., Acta Neuropathol. 53(1), 1981
PMID: 7211201
Bone marrow transplantation corrects the enzyme defect in neurons of the central nervous system in a lysosomal storage disease.
Walkley SU, Thrall MA, Dobrenis K, Huang M, March PA, Siegel DA, Wurzelmann S., Proc. Natl. Acad. Sci. U.S.A. 91(8), 1994
PMID: 8159689
Secondary lipid accumulation in lysosomal disease.
Walkley SU, Vanier MT., Biochim. Biophys. Acta 1793(4), 2009
PMID: 19111580
[Effect of the injection of Triton WR 1339 on the hepatic lysosomes of the rat.]
WATTIAUX R, WIBO M, BAUDHUIN P., Arch. Int. Physiol. Biochim. 71(), 1963
PMID: 13999241
Mannose 6-phosphate receptors, Niemann-Pick C2 protein, and lysosomal cholesterol accumulation.
Willenborg M, Schmidt CK, Braun P, Landgrebe J, von Figura K, Saftig P, Eskelinen EL., J. Lipid Res. 46(12), 2005
PMID: 16177447
Nucleolin: acharan sulfate-binding protein on the surface of cancer cells.
Joo EJ, ten Dam GB, van Kuppevelt TH, Toida T, Linhardt RJ, Kim YS., Glycobiology 15(1), 2005
PMID: 15329357

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 19884343
PubMed | Europe PMC

Search this title in

Google Scholar