Two differentially expressed MATE factor genes from apple complement the Arabidopsis transparent testa12 mutant

Frank S, Keck M, Sagasser M, Niehaus K, Weisshaar B, Stracke R (2011)
Plant Biology 13(1): 42-50.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
Proanthocyanidins (PAs) are a class of flavonoids with numerous functions in plant ecology and development, including protection against microbial infection, animal foraging and damage by UV light. PAs are also beneficial in the human diet and livestock farming, preventing diseases of the cardiovascular system and lowering the risk of cancer, asthma and diabetes. Apples (Malus x domestica Borkh.) are naturally rich in flavonoids, but the flavonoid content and composition varies significantly between cultivars. In this work, we applied knowledge from the model plant Arabidopsis thaliana, for which the main features of flavonoid biosynthesis have been elucidated, to investigate PA accumulation in apple. We identified functional homologues of the Multidrug And Toxic compound Extrusion (MATE) gene TRANSPARENT TESTA12 from A. thaliana using a comparative genomics approach. MdMATE1 and MdMATE2 were differentially expressed, and the function of the encoded proteins was verified by complementation of the respective A. thaliana mutant. In addition, MdMATE genes have a different gene structure in comparison to homologues from other species. Based on our findings, we propose that MdMATE1 and MdMATE2 are vacuolar flavonoid/H(+) -antiporters, active in PA accumulating cells of apple fruit. The identification of these flavonoid transporter genes expands our understanding of secondary metabolite biosynthesis and transport in apple, and is a prerequisite to improve the nutritional value of apples and apple-derived beverages.
Erscheinungsjahr
Zeitschriftentitel
Plant Biology
Band
13
Zeitschriftennummer
1
Seite
42-50
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Frank S, Keck M, Sagasser M, Niehaus K, Weisshaar B, Stracke R. Two differentially expressed MATE factor genes from apple complement the Arabidopsis transparent testa12 mutant. Plant Biology. 2011;13(1):42-50.
Frank, S., Keck, M., Sagasser, M., Niehaus, K., Weisshaar, B., & Stracke, R. (2011). Two differentially expressed MATE factor genes from apple complement the Arabidopsis transparent testa12 mutant. Plant Biology, 13(1), 42-50. doi:10.1111/j.1438-8677.2010.00350.x
Frank, S., Keck, M., Sagasser, M., Niehaus, K., Weisshaar, B., and Stracke, R. (2011). Two differentially expressed MATE factor genes from apple complement the Arabidopsis transparent testa12 mutant. Plant Biology 13, 42-50.
Frank, S., et al., 2011. Two differentially expressed MATE factor genes from apple complement the Arabidopsis transparent testa12 mutant. Plant Biology, 13(1), p 42-50.
S. Frank, et al., “Two differentially expressed MATE factor genes from apple complement the Arabidopsis transparent testa12 mutant”, Plant Biology, vol. 13, 2011, pp. 42-50.
Frank, S., Keck, M., Sagasser, M., Niehaus, K., Weisshaar, B., Stracke, R.: Two differentially expressed MATE factor genes from apple complement the Arabidopsis transparent testa12 mutant. Plant Biology. 13, 42-50 (2011).
Frank, Sandra, Keck, Matthias, Sagasser, Martin, Niehaus, Karsten, Weisshaar, Bernd, and Stracke, Ralf. “Two differentially expressed MATE factor genes from apple complement the Arabidopsis transparent testa12 mutant”. Plant Biology 13.1 (2011): 42-50.

5 Zitationen in Europe PMC

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Global analysis of the MATE gene family of metabolite transporters in tomato.
Santos ALD, Chaves-Silva S, Yang L, Maia LGS, Chalfun-Júnior A, Sinharoy S, Zhao J, Benedito VA., BMC Plant Biol 17(1), 2017
PMID: 29084510
The similar and different evolutionary trends of MATE family occurred between rice and Arabidopsis thaliana.
Wang L, Bei X, Gao J, Li Y, Yan Y, Hu Y., BMC Plant Biol 16(1), 2016
PMID: 27669820
VvMATE1 and VvMATE2 encode putative proanthocyanidin transporters expressed during berry development in Vitis vinifera L.
Pérez-Díaz R, Ryngajllo M, Pérez-Díaz J, Peña-Cortés H, Casaretto JA, González-Villanueva E, Ruiz-Lara S., Plant Cell Rep 33(7), 2014
PMID: 24700246

59 References

Daten bereitgestellt von Europe PubMed Central.

Clustal W and Clustal X version 2.0.
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG., Bioinformatics 23(21), 2007
PMID: 17846036
Secondary transport as an efficient membrane transport mechanism for plant secondary metabolites
Yazaki, Phytochemistry Reviews 7(3), 2008
Global gene expression analysis of apple fruit development from the floral bud to ripe fruit.
Janssen BJ, Thodey K, Schaffer RJ, Alba R, Balakrishnan L, Bishop R, Bowen JH, Crowhurst RN, Gleave AP, Ledger S, McArtney S, Pichler FB, Snowden KC, Ward S., BMC Plant Biol. 8(), 2008
PMID: 18279528
Location of the proanthocyanidins in the barley grain
Aastrup, Carlsberg Research Communications 49(1), 1984
TRANSPARENT TESTA 12 genes from Brassica napus and parental species: cloning, evolution, and differential involvement in yellow seed trait.
Chai YR, Lei B, Huang HL, Li JN, Yin JM, Tang ZL, Wang R, Chen L., Mol. Genet. Genomics 281(1), 2009
PMID: 19018571
Grapevine MATE-type proteins act as vacuolar H+-dependent acylated anthocyanin transporters.
Gomez C, Terrier N, Torregrosa L, Vialet S, Fournier-Level A, Verries C, Souquet JM, Mazauric JP, Klein M, Cheynier V, Ageorges A., Plant Physiol. 150(1), 2009
PMID: 19297587
Mutations in UDP-Glucose:sterol glucosyltransferase in Arabidopsis cause transparent testa phenotype and suberization defect in seeds.
DeBolt S, Scheible WR, Schrick K, Auer M, Beisson F, Bischoff V, Bouvier-Nave P, Carroll A, Hematy K, Li Y, Milne J, Nair M, Schaller H, Zemla M, Somerville C., Plant Physiol. 151(1), 2009
PMID: 19641030

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