Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum

Youn J-W, Jolkver E, Kramer R, Marin K, Wendisch VF (2009)
Journal of Bacteriology 191(17): 5480-5488.

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Transporters of the dicarboxylate amino acid-cation symporter family often mediate uptake of C-4-dicarboxylates, such as succinate or L-malate, in bacteria. A member of this family, dicarboxylate transporter A (DctA) from Corynebacterium glutamicum, was characterized to catalyze uptake of the C-4-dicarboxylates succinate, fumarate, and L-malate, which was inhibited by oxaloacetate, 2-oxoglutarate, and glyoxylate. DctA activity was not affected by sodium availability but was dependent on the electrochemical proton potential. Efficient growth of C. glutamicum in minimal medium with succinate, fumarate, or L-malate as the sole carbon source required high dctA expression levels due either to a promoter-up mutation identified in a spontaneous mutant or to ectopic overexpression. Mutant analysis indicated that DctA and DccT, a C-4-dicarboxylate divalent anion/sodium symporter-type transporter, are the only transporters for succinate, fumarate, and L-malate in C. glutamicum.
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Youn J-W, Jolkver E, Kramer R, Marin K, Wendisch VF. Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum. Journal of Bacteriology. 2009;191(17):5480-5488.
Youn, J. - W., Jolkver, E., Kramer, R., Marin, K., & Wendisch, V. F. (2009). Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum. Journal of Bacteriology, 191(17), 5480-5488. doi:10.1128/Jb.00640-09
Youn, J. - W., Jolkver, E., Kramer, R., Marin, K., and Wendisch, V. F. (2009). Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum. Journal of Bacteriology 191, 5480-5488.
Youn, J.-W., et al., 2009. Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum. Journal of Bacteriology, 191(17), p 5480-5488.
J.-W. Youn, et al., “Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum”, Journal of Bacteriology, vol. 191, 2009, pp. 5480-5488.
Youn, J.-W., Jolkver, E., Kramer, R., Marin, K., Wendisch, V.F.: Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum. Journal of Bacteriology. 191, 5480-5488 (2009).
Youn, Jung-Won, Jolkver, E., Kramer, R., Marin, K., and Wendisch, Volker F. “Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum”. Journal of Bacteriology 191.17 (2009): 5480-5488.
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27 Citations in Europe PMC

Data provided by Europe PubMed Central.

Identification of the membrane protein SucE and its role in succinate transport in Corynebacterium glutamicum.
Huhn S, Jolkver E, Krämer R, Marin K., Appl Microbiol Biotechnol 89(2), 2011
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Biochemical characterization of the C4-dicarboxylate transporter DctA from Bacillus subtilis.
Groeneveld M, Weme RG, Duurkens RH, Slotboom DJ., J Bacteriol 192(11), 2010
PMID: 20363944

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