Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum

Youn J-W, Jolkver E, Kramer R, Marin K, Wendisch VF (2009)
Journal of Bacteriology 191(17): 5480-5488.

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Abstract
Transporters of the dicarboxylate amino acid-cation symporter family often mediate uptake of C-4-dicarboxylates, such as succinate or L-malate, in bacteria. A member of this family, dicarboxylate transporter A (DctA) from Corynebacterium glutamicum, was characterized to catalyze uptake of the C-4-dicarboxylates succinate, fumarate, and L-malate, which was inhibited by oxaloacetate, 2-oxoglutarate, and glyoxylate. DctA activity was not affected by sodium availability but was dependent on the electrochemical proton potential. Efficient growth of C. glutamicum in minimal medium with succinate, fumarate, or L-malate as the sole carbon source required high dctA expression levels due either to a promoter-up mutation identified in a spontaneous mutant or to ectopic overexpression. Mutant analysis indicated that DctA and DccT, a C-4-dicarboxylate divalent anion/sodium symporter-type transporter, are the only transporters for succinate, fumarate, and L-malate in C. glutamicum.
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Youn J-W, Jolkver E, Kramer R, Marin K, Wendisch VF. Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum. Journal of Bacteriology. 2009;191(17):5480-5488.
Youn, J. - W., Jolkver, E., Kramer, R., Marin, K., & Wendisch, V. F. (2009). Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum. Journal of Bacteriology, 191(17), 5480-5488.
Youn, J. - W., Jolkver, E., Kramer, R., Marin, K., and Wendisch, V. F. (2009). Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum. Journal of Bacteriology 191, 5480-5488.
Youn, J.-W., et al., 2009. Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum. Journal of Bacteriology, 191(17), p 5480-5488.
J.-W. Youn, et al., “Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum”, Journal of Bacteriology, vol. 191, 2009, pp. 5480-5488.
Youn, J.-W., Jolkver, E., Kramer, R., Marin, K., Wendisch, V.F.: Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum. Journal of Bacteriology. 191, 5480-5488 (2009).
Youn, Jung-Won, Jolkver, E., Kramer, R., Marin, K., and Wendisch, Volker F. “Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum”. Journal of Bacteriology 191.17 (2009): 5480-5488.
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