Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays

Wendisch VF, Zimmer DP, Khodursky A, Peter B, Cozzarelli N, Kustu S (2001)
Analytical Biochemistry 290(2): 205-213.

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Abstract
Bacterial messenger RNA (mRNA) is not coherently polyadenylated, whereas mRNA of Eukarya can be separated from stable RNAs by virtue of polyadenylated 3 ' -termini, We have developed a method to isolate Escherichia coil mRNA by polyadenylating it in crude cell extracts with E. coil poly(A) polymerase I and purifying it by oligo(dT) chromatography. Differences in lacZRNA levels were similar with purified mRNA and total RNA in dot blot hydridizations for cultures grown with or without gratuitous induction of the lactose operon, More broadly, changes in gene expression upon induction were similar when cDNAs primed from mRNA or total RNA with random hexanucleotides were hydridized to DNA microarrays for the E. coil genome. Comparable signal intensities were obtained with only 1% as much oligo(dT)-purified mRNA as total RNA, and hence in vitro poly(A) tailing appears to be selective for mRNA. These and additional studies of genome-wide expression with DNA microarrays provide evidence that in vitro poly(A) tailing works universally for E. coil mRNAs, (C) 2001 Academic Press.
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Wendisch VF, Zimmer DP, Khodursky A, Peter B, Cozzarelli N, Kustu S. Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays. Analytical Biochemistry. 2001;290(2):205-213.
Wendisch, V. F., Zimmer, D. P., Khodursky, A., Peter, B., Cozzarelli, N., & Kustu, S. (2001). Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays. Analytical Biochemistry, 290(2), 205-213.
Wendisch, V. F., Zimmer, D. P., Khodursky, A., Peter, B., Cozzarelli, N., and Kustu, S. (2001). Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays. Analytical Biochemistry 290, 205-213.
Wendisch, V.F., et al., 2001. Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays. Analytical Biochemistry, 290(2), p 205-213.
V.F. Wendisch, et al., “Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays”, Analytical Biochemistry, vol. 290, 2001, pp. 205-213.
Wendisch, V.F., Zimmer, D.P., Khodursky, A., Peter, B., Cozzarelli, N., Kustu, S.: Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays. Analytical Biochemistry. 290, 205-213 (2001).
Wendisch, Volker F., Zimmer, D. P., Khodursky, A., Peter, B., Cozzarelli, N., and Kustu, S. “Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays”. Analytical Biochemistry 290.2 (2001): 205-213.
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34 References

Data provided by Europe PubMed Central.

NtrC-controlled genes of Escherichia coli: Scavenging as a defense against nitrogen limitation
Zimmer, Proc. Natl. Acad. Sci. USA (), 2000
Messenger RNA translation state: the second dimension of high-throughput expression screening.
Zong Q, Schummer M, Hood L, Morris DR., Proc. Natl. Acad. Sci. U.S.A. 96(19), 1999
PMID: 10485877
Exploring drug-induced alterations in gene expression in Mycobacterium tuberculosis by microarray hybridization
Wilson, Proc. Natl. Acad. Sci. USA 185(), 1999

AUTHOR UNKNOWN, 0
DNA sequence of the lactose operon: the lacA gene and the transcriptional termination region.
Hediger MA, Johnson DF, Nierlich DP, Zabin I., Proc. Natl. Acad. Sci. U.S.A. 82(19), 1985
PMID: 3901000
Intermediates in the degradation of mRNA from the lactose operon of Escherichia coli.
McCormick JR, Zengel JM, Lindahl L., Nucleic Acids Res. 19(10), 1991
PMID: 1710346
Cloning and promoter identification of the iron-regulated cir gene of Escherichia coli.
Griggs DW, Tharp BB, Konisky J., J. Bacteriol. 169(12), 1987
PMID: 3316180

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