Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays

Wendisch VF, Zimmer DP, Khodursky A, Peter B, Cozzarelli N, Kustu S (2001)
Analytical Biochemistry 290(2): 205-213.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Bacterial messenger RNA (mRNA) is not coherently polyadenylated, whereas mRNA of Eukarya can be separated from stable RNAs by virtue of polyadenylated 3 ' -termini, We have developed a method to isolate Escherichia coil mRNA by polyadenylating it in crude cell extracts with E. coil poly(A) polymerase I and purifying it by oligo(dT) chromatography. Differences in lacZRNA levels were similar with purified mRNA and total RNA in dot blot hydridizations for cultures grown with or without gratuitous induction of the lactose operon, More broadly, changes in gene expression upon induction were similar when cDNAs primed from mRNA or total RNA with random hexanucleotides were hydridized to DNA microarrays for the E. coil genome. Comparable signal intensities were obtained with only 1% as much oligo(dT)-purified mRNA as total RNA, and hence in vitro poly(A) tailing appears to be selective for mRNA. These and additional studies of genome-wide expression with DNA microarrays provide evidence that in vitro poly(A) tailing works universally for E. coil mRNAs, (C) 2001 Academic Press.
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Zeitschriftentitel
Analytical Biochemistry
Band
290
Zeitschriftennummer
2
Seite
205-213
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Wendisch VF, Zimmer DP, Khodursky A, Peter B, Cozzarelli N, Kustu S. Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays. Analytical Biochemistry. 2001;290(2):205-213.
Wendisch, V. F., Zimmer, D. P., Khodursky, A., Peter, B., Cozzarelli, N., & Kustu, S. (2001). Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays. Analytical Biochemistry, 290(2), 205-213. doi:10.1006/abio.2000.4982
Wendisch, V. F., Zimmer, D. P., Khodursky, A., Peter, B., Cozzarelli, N., and Kustu, S. (2001). Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays. Analytical Biochemistry 290, 205-213.
Wendisch, V.F., et al., 2001. Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays. Analytical Biochemistry, 290(2), p 205-213.
V.F. Wendisch, et al., “Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays”, Analytical Biochemistry, vol. 290, 2001, pp. 205-213.
Wendisch, V.F., Zimmer, D.P., Khodursky, A., Peter, B., Cozzarelli, N., Kustu, S.: Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays. Analytical Biochemistry. 290, 205-213 (2001).
Wendisch, Volker F., Zimmer, D. P., Khodursky, A., Peter, B., Cozzarelli, N., and Kustu, S. “Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays”. Analytical Biochemistry 290.2 (2001): 205-213.

37 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Bacterial mRNA purification by magnetic capture-hybridization method.
Pang X, Zhou D, Song Y, Pei D, Wang J, Guo Z, Yang R., Microbiol Immunol 48(2), 2004
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Genome image programs: visualization and interpretation of Escherichia coli microarray experiments.
Zimmer DP, Paliy O, Thomas B, Gyaneshwar P, Kustu S., Genetics 167(4), 2004
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DNA microarray analyses of the long-term adaptive response of Escherichia coli to acetate and propionate.
Polen T, Rittmann D, Wendisch VF, Sahm H., Appl Environ Microbiol 69(3), 2003
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Global expression profiling and physiological characterization of Corynebacterium glutamicum grown in the presence of L-valine.
Lange C, Rittmann D, Wendisch VF, Bott M, Sahm H., Appl Environ Microbiol 69(5), 2003
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The phosphate starvation stimulon of Corynebacterium glutamicum determined by DNA microarray analyses.
Ishige T, Krause M, Bott M, Wendisch VF, Sahm H., J Bacteriol 185(15), 2003
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Physiological studies of Escherichia coli strain MG1655: growth defects and apparent cross-regulation of gene expression.
Soupene E, van Heeswijk WC, Plumbridge J, Stewart V, Bertenthal D, Lee H, Prasad G, Paliy O, Charernnoppakul P, Kustu S., J Bacteriol 185(18), 2003
PMID: 12949114
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Rhodius V, Van Dyk TK, Gross C, LaRossa RA., Annu Rev Microbiol 56(), 2002
PMID: 12142487
LrhA as a new transcriptional key regulator of flagella, motility and chemotaxis genes in Escherichia coli.
Lehnen D, Blumer C, Polen T, Wackwitz B, Wendisch VF, Unden G., Mol Microbiol 45(2), 2002
PMID: 12123461
Applications of DNA microarrays in microbial systems.
Ye RW, Wang T, Bedzyk L, Croker KM., J Microbiol Methods 47(3), 2001
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