Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production

Stansen C, Uy D, Delaunay S, Eggeling L, Goergen JL, Wendisch VF (2005)
Applied and Environmental Microbiology 71(10): 5920-5928.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Gene expression changes of glutamate-producing Corynebacterium glutamicum were identified in transcriptome comparisons by DNA microarray analysis. During glutamate production induced by a temperature shift, C glutamicum strain 2262 showed significantly higher mRNA levels of the NCgl2816 and NCgl2817 genes than its non-glutamate- producing derivative 2262NP. Reverse transcription-PCR analysis showed that the two genes together constitute an operon. NCgl2816 putatively codes for a lactate permease, while NCgl2817 was demonstrated to encode quinone-dependent L-lactate dehydrogenase, which was named LldD. C. glutamicum LldD displayed Michaelis-Menten kinetics for the substrate L-lactate with a K-m of about 0.51 mM. The specific activity of LIdD was about 10-fold higher during growth on L-lactate or on an L-lactate-glucose mixture than during growth on glucose, D-lactate, or pyruvate, while the specific activity of quinone-dependent D-lactate dehydrogenase differed little with the carbon source. RNA levels of NCg12816 and ildD were about 18-fold higher during growth on L-lactate than on pyruvate. Disruption of the NCg12816-ildD operon resulted in loss of the ability to utilize L-lactate as the sole carbon source. Expression of ildD restored L-lactate utilization, indicating that the function of the permease gene NCgl2816 is dispensable, while LIdD is essential, for growth of C. glutamicum on L-lactate.
Erscheinungsjahr
Zeitschriftentitel
Applied and Environmental Microbiology
Band
71
Zeitschriftennummer
10
Seite
5920-5928
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Stansen C, Uy D, Delaunay S, Eggeling L, Goergen JL, Wendisch VF. Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production. Applied and Environmental Microbiology. 2005;71(10):5920-5928.
Stansen, C., Uy, D., Delaunay, S., Eggeling, L., Goergen, J. L., & Wendisch, V. F. (2005). Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production. Applied and Environmental Microbiology, 71(10), 5920-5928. doi:10.1128/Aem.71.10.5920-5928.2005
Stansen, C., Uy, D., Delaunay, S., Eggeling, L., Goergen, J. L., and Wendisch, V. F. (2005). Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production. Applied and Environmental Microbiology 71, 5920-5928.
Stansen, C., et al., 2005. Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production. Applied and Environmental Microbiology, 71(10), p 5920-5928.
C. Stansen, et al., “Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production”, Applied and Environmental Microbiology, vol. 71, 2005, pp. 5920-5928.
Stansen, C., Uy, D., Delaunay, S., Eggeling, L., Goergen, J.L., Wendisch, V.F.: Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production. Applied and Environmental Microbiology. 71, 5920-5928 (2005).
Stansen, C., Uy, D., Delaunay, S., Eggeling, L., Goergen, J. L., and Wendisch, Volker F. “Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production”. Applied and Environmental Microbiology 71.10 (2005): 5920-5928.

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