Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis

Polen T, Schluesener D, Poetsch A, Bott M, Wendisch VF (2007)
Fems Microbiology Letters 273(1): 109-119.

Download
No fulltext has been uploaded. References only!
Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
Corynebacterium glutamicum grows aerobically on a variety of carbohydrates and organic acids as single or combined sources of carbon and energy. To characterize the citrate utilization in C. glutamicum on a genomewide scale, a comparative analysis was carried out by combining transcriptome and proteome analysis. In cells grown on citrate, transcriptome analysis revealed highest expression changes for two different citrate-uptake systems encoded by citM and tctCBA, whereas genes encoding uptake systems for the glucose- (ptsG), sucrose- (ptsS) and fructose- (ptsF) specific PTS components and permeases for gluconate (gntP) and glutamate (gluC) displayed decreased mRNA levels in citrate-grown cells. This pattern was also observed when cells grown in Luria-Bertani (LB) medium plus citrate were compared with cells grown in LB medium, indicating some kind of catabolite repression. Genes encoding enzymes of the tricarboxylic acid cycle (aconitase, succinyl-CoA synthetase, succinate dehydrogenase and fumarase), malic enzyme, PEP carboxykinase, gluconeogenic glyceraldehyde-3-phosphate dehydrogenase and ATP synthase displayed increased expression in cells grown on citrate. Accordingly, proteome analysis revealed elevated protein levels of these enzymes and showed a good correlation with the mRNA levels. In conclusion, this study revealed the citrate stimulon in C. glutamicum and the regulated central metabolic genes when grown on citrate.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Polen T, Schluesener D, Poetsch A, Bott M, Wendisch VF. Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters. 2007;273(1):109-119.
Polen, T., Schluesener, D., Poetsch, A., Bott, M., & Wendisch, V. F. (2007). Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters, 273(1), 109-119. doi:10.1111/j.1574-6968.2007.00793.x
Polen, T., Schluesener, D., Poetsch, A., Bott, M., and Wendisch, V. F. (2007). Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters 273, 109-119.
Polen, T., et al., 2007. Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters, 273(1), p 109-119.
T. Polen, et al., “Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis”, Fems Microbiology Letters, vol. 273, 2007, pp. 109-119.
Polen, T., Schluesener, D., Poetsch, A., Bott, M., Wendisch, V.F.: Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters. 273, 109-119 (2007).
Polen, T., Schluesener, D., Poetsch, A., Bott, M., and Wendisch, Volker F. “Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis”. Fems Microbiology Letters 273.1 (2007): 109-119.
This data publication is cited in the following publications:
This publication cites the following data publications:

36 Citations in Europe PMC

Data provided by Europe PubMed Central.

The yctCBA operon of Yersinia ruckeri, involved in in vivo citrate uptake, is not required for virulence.
Navais R, Méndez J, Reimundo P, Pérez-Pascual D, Gómez E, Guijarro JA., Appl Environ Microbiol 77(3), 2011
PMID: 21131526
Phosphotransferase system-independent glucose utilization in corynebacterium glutamicum by inositol permeases and glucokinases.
Lindner SN, Seibold GM, Henrich A, Krämer R, Wendisch VF., Appl Environ Microbiol 77(11), 2011
PMID: 21478323
Evolution: like any other science it is predictable.
Morris SC., Philos Trans R Soc Lond B Biol Sci 365(1537), 2010
PMID: 20008391
Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate.
Kato O, Youn JW, Stansen KC, Matsui D, Oikawa T, Wendisch VF., BMC Microbiol 10(), 2010
PMID: 21159175
Physiological adaptation of Corynebacterium glutamicum to benzoate as alternative carbon source - a membrane proteome-centric view.
Haussmann U, Qi SW, Wolters D, Rögner M, Liu SJ, Poetsch A., Proteomics 9(14), 2009
PMID: 19639586
Characterization of the dicarboxylate transporter DctA in Corynebacterium glutamicum.
Youn JW, Jolkver E, Krämer R, Marin K, Wendisch VF., J Bacteriol 191(17), 2009
PMID: 19581365
Identification and characterization of the dicarboxylate uptake system DccT in Corynebacterium glutamicum.
Youn JW, Jolkver E, Krämer R, Marin K, Wendisch VF., J Bacteriol 190(19), 2008
PMID: 18658264
Response of the cytoplasmic and membrane proteome of Corynebacterium glutamicum ATCC 13032 to pH changes.
Barriuso-Iglesias M, Schluesener D, Barreiro C, Poetsch A, Martín JF., BMC Microbiol 8(), 2008
PMID: 19091079

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 17559405
PubMed | Europe PMC

Search this title in

Google Scholar