C-3-carboxylation as an anaplerotic reaction in phosphoenolpyruvate carboxylase-deficient Corynebacterium glutamicum

Peters-Wendisch P, Wendisch VF, deGraaf AA, Eikmanns BJ, Sahm H (1996)
Archives of Microbiology 165(6): 387-396.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
Phosphoenolpyruvate carboxylase (PEPCx) has recently been found to be dispensable as an anaplerotic enzyme for growth and lysine production of Corynebacterium glutamicum. To clarify the role of the glyoxylate cycle as a possible alternative anaplerotic sequence, defined PEPCx- and isocitrate-lyase (ICL)-negative double mutants of C. glutamicum wild-type and of the L-lysine-producing strain MH20-22B were constructed by disruption of the respective genes. Analysis of these mutants revealed that the growth on glucose and the lysine productivity were identical to that of the parental strains. These results show that PEPCx and the glyoxylate cycle are not essential for growth of C. glutamicum on glucose and for lysine production and prove the presence of another anaplerotic reaction in this organism. To study the anaplerotic pathways in C. glutamicum further, (HCO3-)-C-13-labeling experiments were performed with cells of the wild-type and a PEPCx-negative strain growing on glucose. Proton nuclear magnetic resonance analysis of threonine isolated from cell protein of both strains revealed the same labeling pattern: about 37% C-13 enrichment in C-4 and 3.5% C-13 enrichment in C-1. Since the carbon backbone of threonine corresponds to that of oxaloacetate, the label in C-4 of threonine positively identifies the anaplerotic pathway as a C-3-carboxylation reaction that also takes place in the absence of PEPCx.
Publishing Year
ISSN
PUB-ID

Cite this

Peters-Wendisch P, Wendisch VF, deGraaf AA, Eikmanns BJ, Sahm H. C-3-carboxylation as an anaplerotic reaction in phosphoenolpyruvate carboxylase-deficient Corynebacterium glutamicum. Archives of Microbiology. 1996;165(6):387-396.
Peters-Wendisch, P., Wendisch, V. F., deGraaf, A. A., Eikmanns, B. J., & Sahm, H. (1996). C-3-carboxylation as an anaplerotic reaction in phosphoenolpyruvate carboxylase-deficient Corynebacterium glutamicum. Archives of Microbiology, 165(6), 387-396.
Peters-Wendisch, P., Wendisch, V. F., deGraaf, A. A., Eikmanns, B. J., and Sahm, H. (1996). C-3-carboxylation as an anaplerotic reaction in phosphoenolpyruvate carboxylase-deficient Corynebacterium glutamicum. Archives of Microbiology 165, 387-396.
Peters-Wendisch, P., et al., 1996. C-3-carboxylation as an anaplerotic reaction in phosphoenolpyruvate carboxylase-deficient Corynebacterium glutamicum. Archives of Microbiology, 165(6), p 387-396.
P. Peters-Wendisch, et al., “C-3-carboxylation as an anaplerotic reaction in phosphoenolpyruvate carboxylase-deficient Corynebacterium glutamicum”, Archives of Microbiology, vol. 165, 1996, pp. 387-396.
Peters-Wendisch, P., Wendisch, V.F., deGraaf, A.A., Eikmanns, B.J., Sahm, H.: C-3-carboxylation as an anaplerotic reaction in phosphoenolpyruvate carboxylase-deficient Corynebacterium glutamicum. Archives of Microbiology. 165, 387-396 (1996).
Peters-Wendisch, Petra, Wendisch, Volker F., deGraaf, A. A., Eikmanns, B. J., and Sahm, H. “C-3-carboxylation as an anaplerotic reaction in phosphoenolpyruvate carboxylase-deficient Corynebacterium glutamicum”. Archives of Microbiology 165.6 (1996): 387-396.
This data publication is cited in the following publications:
This publication cites the following data publications:

19 Citations in Europe PMC

Data provided by Europe PubMed Central.

Engineering biotin prototrophic Corynebacterium glutamicum strains for amino acid, diamine and carotenoid production.
Peters-Wendisch P, Götker S, Heider SA, Komati Reddy G, Nguyen AQ, Stansen KC, Wendisch VF., J Biotechnol 192 Pt B(), 2014
PMID: 24486440
The methylotrophic Bacillus methanolicus MGA3 possesses two distinct fructose 1,6-bisphosphate aldolases.
Stolzenberger J, Lindner SN, Wendisch VF., Microbiology 159(pt 8), 2013
PMID: 23760818
Biotin protein ligase from Corynebacterium glutamicum: role for growth and L: -lysine production.
Peters-Wendisch P, Stansen KC, Götker S, Wendisch VF., Appl Microbiol Biotechnol 93(6), 2012
PMID: 22159614
Phosphotransferase system-independent glucose utilization in corynebacterium glutamicum by inositol permeases and glucokinases.
Lindner SN, Seibold GM, Henrich A, Krämer R, Wendisch VF., Appl Environ Microbiol 77(11), 2011
PMID: 21478323
Emerging Corynebacterium glutamicum systems biology.
Wendisch VF, Bott M, Kalinowski J, Oldiges M, Wiechert W., J Biotechnol 124(1), 2006
PMID: 16406159
Cometabolism of a nongrowth substrate: L-serine utilization by Corynebacterium glutamicum.
Netzer R, Peters-Wendisch P, Eggeling L, Sahm H., Appl Environ Microbiol 70(12), 2004
PMID: 15574911
Pathway analysis and metabolic engineering in Corynebacterium glutamicum.
Sahm H, Eggeling L, de Graaf AA., Biol Chem 381(9-10), 2000
PMID: 11076021
Metabolic analysis of glutamate production by Corynebacterium glutamicum.
Gourdon P, Lindley ND., Metab Eng 1(3), 1999
PMID: 10937937
Importance of phosphoenolpyruvate carboxylase of Corynebacterium glutamicum during the temperature triggered glutamic acid fermentation.
Delaunay S, Uy D, Baucher MF, Engasser JM, Guyonvarch A, Goergen JL., Metab Eng 1(4), 1999
PMID: 10937826
Efflux of compatible solutes in Corynebacterium glutamicum mediated by osmoregulated channel activity.
Ruffert S, Lambert C, Peter H, Wendisch VF, Krämer R., Eur J Biochem 247(2), 1997
PMID: 9266699
Response of the central metabolism of Corynebacterium glutamicum to different flux burdens.
Marx A, Striegel K, de Graaf AA, Sahm H, Eggeling L., Biotechnol Bioeng 56(2), 1997
PMID: 18636622

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 8661932
PubMed | Europe PMC

Search this title in

Google Scholar